Abstract：
:Acquired resistance to chemotherapy is a major limitation for the successful treatment of lung cancer. Previously, we and others showed that formation of tumor spheres is associated with chemotherapy resistance in lung cancer cells, but the underlying mechanisms remained largely unknown. In the current study, we show that mitochondrial activity is significantly higher in A549 tumor spheres versus monolayer cells, establishing mitochondria as a putative target for antitumor therapy. To this end, we designed a peptide nucleic acids (PNAs) coupled with triphenylphosphonium (TPP) to target the displacement loop (D-loop) regulatory region of mitochondrial DNA (PNA-mito). Treatment with PNA-mito significantly disrupted mitochondrial gene expression, inhibited membrane potential and mitochondria fusion, resulting in proliferation inhibition and cell death. Consistently, in mouse xenograft models, PNA-mito could efficiently inhibit mitochondrial gene expression and block tumor growth. Treatment with a low dose of PNA-mito could significantly enhance the chemotoxicity of cisplatin (CDDP) in drug-resistant A549 tumor spheres. These results establish mitochondria-targeting PNAs as a novel strategy to enhance the accumulative therapeutic outcome of lung cancer.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Chen SS,Tu XY,Xie LX,Xiong LP,Song J,Ye XQsubject
Has Abstractpub_date
2018-09-15 00:00:00pages
2940-2948issue
9issn
1943-8141journal_volume
10pub_type
杂志文章abstract：:We tested the hypothesis that human induced pluripotent stem cell-derived mesenchymal stem cell (iPSC-MSC) therapy could effectively reduce brain-infarct volume (BIV) and improve neurological function in rat after acute intracranial hemorrhage (ICH) induced by a weight-drop device. Adult-male SD rats (n=40) were equal...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-09-15 00:00:00
abstract：:Rubus alceaefolius Poir. has been used for the treatment of nasopharyngeal carcinoma (NPC) in China for many years. Euscaphic acid is an active component of Rubus alceaefolius Poir. However, the mechanism of action of euscaphic acid in NPC remains unclear. In this study, Euscaphic acid inhibited the proliferation of N...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-04-15 00:00:00
abstract：:Although microRNAs (miRNAs) play an important role in esophageal squamous cell carcinoma (ESCC), their roles in radiotherapy response remain unexplored. Our study aims to investigate whether plasma miRNAs can be used as predictors of radiotherapy outcome in ESCC patients. We selected nine miRNAs, which were reported t...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) act critical roles in many biological processes, including cell proliferation, apoptosis, development, invasion and migration. LncRNA maternally expressed gene 3 (MEG3) is found to be downregulated in several tumors; however, its role in the atherosclerosis is still unknown. In the prese...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-07-15 00:00:00
abstract：AIMS:This study was to investigate the effect of TUG1 on apoptosis and ECM degradation of human degenerative intervertebral disc nucleus pulposus cells (NPCs) and its mechanism. METHODS:Human degenerative intervertebral disc NP tissues were obtained from 10 patients with lumbar disc herniation (LDH) who underwent lumb...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：:Immune checkpoints are known to contribute to tumor progression by enhancing cancer's ability to evade the immune system and metastasize. Immunotherapies, including monoclonal antibodies, have been developed to target specific immunosuppressive molecules on the membranes of cancer cells and have proven revolutionary i...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2019-02-15 00:00:00
abstract：:Wound repair is an intractable problem in clinic, with limited treatment options. Previous studies have demonstrated the therapeutic potential of Nogo-B in tissue repairs. However, the therapeutic effect of Nogo-B in HUVEC is still unknown. In this study, we examined the benefit of genetically modified Human Umbilical...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-05-15 00:00:00
abstract：:Nonalcoholic steatohepatitis represents a significant and rapidly growing unmet medical need. The development of novel therapies has been hindered in part, by the limitations of existing preclinical models. There is a strong need for physiologically relevant in vivo and in vitro liver fibrosis models that are characte...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:As an autoimmune disease, myasthenia gravis is caused by the dysfunction of neural transmission. Acetylcholine is known to exert its function after entering into synaptic cleft through binding onto postsynaptic membrane. The role of acetylcholine in binding MuSK in myasthenia gravis, however, remains unknown. A total ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-04-15 00:00:00
abstract：:Fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary flavonoid and has been indicated as a novel anti-cancer agent in several types of cancer cells. However, the mechanisms underlying the effect of fisetin in human oral squamous cell carcinoma (OSCC) remain unclear. Here, we report that fisetin significantly inhibits ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-12-15 00:00:00
abstract：:Peripheral neuropathic pain is a complex disease, and treated based on underlying diseases. Emerging evidences suggest that hyperbaric oxygen alleviates neuropathic pain. However, its cellular and molecular mechanism on pain relief is unknown. We hypothesize that hyperbaric oxygen alleviates neuropathic pain via activ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-05-15 00:00:00
abstract：:Background/Introduction: Aberrant expression of Toll like receptors (TLR) plays a vital role in pathogenesis of rheumatoid arthritis (RA). Micro RNAs (miRs) could play important role in the related signaling pathways. The present study was undertaken to establish the link between miR-147 and TLR-7 in rat macrophages (...
journal_title：American journal of translational research
pub_type： 杂志文章,收录出版
doi：
更新日期：2019-04-15 00:00:00
abstract：:Intraoperative auto-transfusion with the use of cell saver systems is routinely used to reduce the rate of packed red blood transfusion in major surgery. Nevertheless some concerns have been raised on possible risks of coagulation disorders. The aim of the study was to analyze the blood processed by the cell saver, re...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:Acuteliver failure (ALF) has a high mortality rate and is characterized by massive hepatocyte destruction. Although microRNAs (miRNAs) play an important role in manyliver diseases, the role of miRNAs in ALF development is unknown. In this study, the murine ALF model was induced by intraperitoneal injection of D-galact...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-11-15 00:00:00
abstract：:In this study, we investigated the biological function of pyruvate kinase M2 (PKM2) and its regulation by deregulated microRNAs (miRNAs) in thyroid cancer (TC). The mRNA and protein expression of PKM2 was examined by quantitative reverse transcription PCR and western blot. The biological role of PKM2 was demonstrated ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-06-15 00:00:00
abstract：:The study was designed to investigate the effect of Nimesulide (NIM) on acute lung injury (ALI) in mice with severe acute pancreatitis (SAP). In our study, caerulein and LPS were employed to establish the ALI mice model induced by SAP. All animals were divided into four groups randomly: control, model (SAP), NIM low a...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-09-15 00:00:00
abstract：:We recently reported a novel anticancer small molecule, designated FL118, which was discovered via high throughput screening (HTS), and followed by hit-lead in vitro and in vivo analysis. FL118 selectively inhibits the expression of four major cancer survival-associated gene products (survivin, Mcl-1, XIAP, and cIAP2)...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:HOE-642 has been shown to provide significant protection in a variety of models of cerebral and myocardial ischemia/reperfusion injury. In this study, we examined the impact of HOE-642, a selective Na+/H+ exchanger 1 inhibitor, with or without hypothermia on neuronal and neuronal mitochondrial function during resuscit...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-05-15 00:00:00
abstract：PURPOSE:Primary intraosseous squamous cell carcinoma (PIOSCC) arising within an odontogenic keratocyst (OKC) is rare malignancy, entailing a poor prognosis for delayed diagnosis. The number of reports concerning this entity is extremely small. The aim of this study is to present the clinical and pathologic characterist...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
abstract：:Nonalcoholic steatohepatitis (NASH) has similar clinical pathological changes to alcoholic hepatitis. It shows increased incidence and young trend year by year. Polyene phosphatidyl choline (PPC) is widely used in clinic for liver disease treatment. The effect and mechanism of PPC on NASH have not been fully elucidate...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-05-15 00:00:00
abstract：:Circular RNAs (circRNAs), a class of endogenous RNAs, are characterized by covalently closed continuous loop without 5' to 3' polarity and polyadenylated tail. Recent studies indicated that circRNAs might play an important role in cancer. However, the function of circRNA in laryngeal squamous cell cancer tissues (LSCC...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
abstract：:Mast cells are important effector cells of the immune system, and mast cell-derived exosomes carrying RNAs play a role in immune regulation. However, the molecular function of mast cell-derived exosomes is currently unknown, and here, we identify differentially expressed genes (DEGs) in mast cells and exosomes. We iso...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-02-15 00:00:00
abstract：:Incidence of nasopharyngeal carcinoma (NPC) has remained high worldwide, posing a serious health problem. MicroRNAs (miRNAs) are a family of about 20-23 nucleotides small non-coding molecules, which play a significant role in NPC. In this study, we explored the molecular mechanisms of miR-130a-3p in inhibiting viabili...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-08-15 00:00:00
abstract：:Principal component analysis (PCA) is a data analysis method that can deal with large volumes of data. Owing to the complexity and volume of the data generated by today's advanced technologies in genomics, pro-teomics, and metabolomics, PCA has become predominant in the medical sciences. Despite its popularity, PCA le...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2011-02-01 00:00:00
abstract：:Pyrroloquinoline quinone (PQQ), considered as an ROS scavenger,could protect mitochondrial activity from damage of oxidative stress. To determine the role of PQQ supplement in rescuing long bone osteoporosis in Bmi-1-/- mice. We fed Bmi-1 knockout mice a diet supplemented with PQQ (BKO+PQQ), BKO mice with normal diet ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-10-15 00:00:00
abstract：BACKGROUND:Atherosclerosis remains a leading cause of cardiology disease worldwide, which vascular smooth muscle cells (VSMCs) proliferation and apoptosis are involved. Increasing evidences have revealed that long non-coding RNAs (lncRNAs) considered to be critical regulatory factors of VSMCs function. However, the mol...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-06-15 00:00:00
abstract：:Growing evidence has revealed that the initiation of various malignancies is closely associated with alternative splicing (AS) events in certain key oncogenes. However, in diffuse large B-cell lymphoma (DLBCL), there is still a great deal to learn about AS variants. In this study, 33,724 AS variant profiles were obtai...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-08-15 00:00:00
abstract：:Intrauterine adhesion (IUA) is a common disease among women after uterus operation. BMSCs are commonly used as a therapeutic agent for IUA treatment, but the underlying mechanism is not fully delineated. Here we showed that BMSCs co-cultured with EPCs promotes proliferative ability and decreases apoptosis ratio of BMS...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:Background: Pericardial patches are frequently used in vascular surgery to close arteriotomies. The early healing of these patches is mediated by attraction of CD34 and ephrinB2-positive cells. Atorvastatin is a commonly used statin drug that promotes healing of cardiovascular injury. We hypothesized that atorvastatin...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:The serine/threonine protein kinase B (PKB, also known as Akt) constitutes an important node in diverse signaling cascades downstream of growth factor receptor tyrosine kinases. Akt plays an essential role in cell survival, growth, migration, proliferation, polarity, and metabolism (lipid and glucose); cell cycle prog...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
