Abstract:
:In this study, we investigated the biological function of pyruvate kinase M2 (PKM2) and its regulation by deregulated microRNAs (miRNAs) in thyroid cancer (TC). The mRNA and protein expression of PKM2 was examined by quantitative reverse transcription PCR and western blot. The biological role of PKM2 was demonstrated through small interfering RNA-mediated knockdown experiments. The regulation of PKM2 by miR-148a and miR-326 was confirmed by western blot, dual luciferase activity assays, and rescue experiments. PKM2 was overexpressed in TC tissues and cell lines. The knockdown of PKM2 in TC cells suppressed cell proliferation, reduced colony formation, and inhibited cell invasion and migration significantly. Luciferase reporter assays revealed that PKM2 is a direct target of two tumor-suppressive miRNAs, miR-148a and miR-326. Re-expressed PKM2 rescued the anticancer effects of miR-148a. Taken together, these data strongly suggest that, apart from gene amplification and mutation, the activation of PKM2 in TC is partly due to the down-regulation of the tumor-suppressive miRNAs miR-148a and miR-326. Thus, PKM2 is overexpressed and plays an oncogenic role in thyroid carcinogenesis.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Yu G,Sun W,Shen Y,Hu Y,Liu H,Li W,Wang Ysubject
Has Abstractpub_date
2018-06-15 00:00:00pages
1793-1801issue
6issn
1943-8141journal_volume
10pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-01-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2019-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2019-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2019-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2017-12-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2015-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2019-07-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2017-11-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2015-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,已发布勘误
doi:
更新日期:2018-12-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2019-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-04-15 00:00:00
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-07-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2018-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2017-06-15 00:00:00