Abstract：
:NSUN5, a gene encodes a cytosine-5 RNA methyltransferase, is rarely mentioned in cancers. Our study is the first one to evaluate the role of NSUN5 in the progression of colorectal cancer. Data from TCGA was used to show the different expression of NSUN5 between CRC tumor tissues and adjacent normal ones. The NSUN5 expression in the tissue microarray was detected by immunohistochemistry (IHC). qRT-PCR was conducted for NSUN5 expression examination in CRC cell lines. Cell proliferation was analyzed by the Celigo machine. GESA and correlation analysis were performed to reveal the possible underlying mechanism. The effects of NSUN5 expression on CRC cell behavior in vitro were analyzed by flow cytometry and β-galactosidase staining. The expression of cell-cycle related proteins were evaluated by western blot. Subcutaneously implanted tumor model was carried out for animal experiment. NSUN5 expression was up-regulated in CRC tumor tissues and cells, and associated with advanced tumor stages (III, IV). NSUN5 could promote cell proliferation, trigger cell cycle arrest in vitro and boost tumor growth in vivo. In addition, knockdown of NSUN5 could lead to a higher expression of Rb and a lower expression of CDK4, CDK6, p-Rb and CCNE1, but made no difference on P21, Bcl-2, caspase3 and C-Caspase3 of CRC cells. Taken together, we identify NSUN5 as a promoter in CRC development via cell cycle regulation.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Jiang Z,Li S,Han MJ,Hu GM,Cheng Psubject
Has Abstractpub_date
2020-07-15 00:00:00pages
3858-3870issue
7issn
1943-8141journal_volume
12pub_type
杂志文章abstract：BACKGROUND:To investigated the peripheral stromal cell conditioned medium (CM) -stimulated c-kit-JAK2-STAT1 pathway in prostate cancer. METHODS:CM harvested from normal prostate peripheral stromal cells was added to DU145 cells. DU145 cell viability and migration were measured by cell counting kit-8 reagent and Transw...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-03-15 00:00:00
abstract：:The histone deacetylase inhibitors, suberoylanilide hydroxamic acid (Vorinostat, Zolinza™) and depsipeptide (Romidepsin, Istodax™) have been approved by the US Food and Drug Administration for the treatment of refractory cutaneous T-cell lymphoma. Numerous histone deacetylase inhibitors are currently undergoing clinic...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Bronchopulmonary dysplasia (BPD) is one of the most common causes of mortality and morbidity in neonatal intensive care units. Persistent inflammation, with an abnormal influx of polymorphonuclear leukocytes (PMNs) followed by monocytes (MONOs), occurs early in the pathogenesis of BPD. Anti-inflammatory therapy with b...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：AIMS:This study was to investigate the effect of TUG1 on apoptosis and ECM degradation of human degenerative intervertebral disc nucleus pulposus cells (NPCs) and its mechanism. METHODS:Human degenerative intervertebral disc NP tissues were obtained from 10 patients with lumbar disc herniation (LDH) who underwent lumb...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：:Gastric cancer (GC) causes nearly one million deaths worldwide each year. However, the molecular pathway of GC development remains unclear. Increasing evidences have shown that microRNAs (miRNAs) are highly associated with tumor development. However, relative little is known about the potential role of miRNAs in gastr...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-09-15 00:00:00
abstract：:Intrauterine adhesion (IUA) is a common disease among women after uterus operation. BMSCs are commonly used as a therapeutic agent for IUA treatment, but the underlying mechanism is not fully delineated. Here we showed that BMSCs co-cultured with EPCs promotes proliferative ability and decreases apoptosis ratio of BMS...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:B-cell lymphoma/leukaemia 11A (BCL11A) is a modulator of foetal-to-adult globin switching and is involved in brain development and normal lymphopoiesis. The three-dimensional structure of BCL11A and its structural domains had not yet been completely determined; hence, this study aimed to elucidate the structural domai...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-06-15 00:00:00
abstract：BACKGROUND:Gastric cancer (GC) is one of the most common digestive tract tumors, and a serious threat to human health. Long non-coding RNA (lncRNA) are involved in many cancers. However, the biological functions of most lncRNAs are unclear. In this study, we investigated the mechanisms by which FLVCR1-AS1 regulated GC ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-02-15 00:00:00
abstract：AIM:Geniposide is an iridoid glycoside isolated from the gardenia plant. It has multiple biological activities. The roles of geniposide in systemic sclerosis (SSc) and in endothelial-to-mesenchymal transition (EndMT) are unclear. We investigated the protective effects of geniposide in a bleomycin-induced SSc mouse mode...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-03-15 00:00:00
abstract：:Xp11.2 translocation/transcription factor E3 (TFE3) gene fusion renal cell carcinoma (Xp11.2 translocation RCC) was first classified as a distinct type of renal tumor by the World Health Organization in 2004. However, its morphology and clinical manifestations often overlap with those of conventional RCCs. Moreover, a...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-01-15 00:00:00
abstract：:Random pattern skin flap transplantation is frequently applied in plastic and reconstructive surgery, but the distal part of skin flaps often suffers necrosis due to ischemia. Astragaloside IV (AS-IV), a natural saponin purified from Astragalus membranaceus, may have beneficial functions for flap survival. In this stu...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-09-15 00:00:00
abstract：:Glioma is an aggressive nervous system tumor with poor prognosis. Although the therapeutic strategies to overcome glioma have been improved largely recent years, the potential mechanism of its carcinogenesis remains largely unclear. The present study aimed to investigate the role of long non-coding RNA HOMEOBOX A11 an...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-10-15 00:00:00
abstract：:The abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are crucial pathological processes that are involved in atherosclerosis. Growing evidence suggests that microRNAs (miRNAs) play critical roles in VSMCs functions. Here, we analyzed the expression of four atherosclerosis-related miRNAs and...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-02-15 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) act critical roles in many biological processes, including cell proliferation, apoptosis, development, invasion and migration. LncRNA maternally expressed gene 3 (MEG3) is found to be downregulated in several tumors; however, its role in the atherosclerosis is still unknown. In the prese...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-07-15 00:00:00
abstract：:Circular RNAs (circRNAs) belong to non-coding RNAs and are known as key regulators in gene regulation. CircRNAs involve in the various biological processes of cancer. However, the functions of circRNAs in renal cell carcinoma (RCC) are still not clear. In this study, the circRNA expression profile was performed in the...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-08-15 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) have been recently reported to be dysregulated and play a critical role in the progression of thyroid cancer. Here, we found that the lncRNA n340790 was highly expressed in human thyroid cancer tissues and was strongly correlated with the clinical characteristics of patients. There was a...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-05-15 00:00:00
abstract：:The effect of different static magnetic field strengths, 1 T or 7 T, on the quality of nitroxyl radical-based magnetic resonance redox imaging (MRRI) was examined. A stable nitroxyl radical, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (MC-PROXYL), was used as a T1 contrast agent. Phantoms and animals were ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-10-15 00:00:00
abstract：:Angiotesin II (Ang II) plays an important role in cardiac remodeling. Fibroblast growth factor inducible-14 (Fn14) is the smallest member of the tumor necrosis factor superfamily of receptors. Currently, little is known about the functional role of Fn14 in the heart. Chiefly, we observe the up-regulation of extracellu...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-12-15 00:00:00
abstract：:Urine-derived stem cells (USCs) are autologous stem cells that exhibit self-renewal ability and multi-lineage differentiation potential. These characteristics make USCs an ideal cell source for hepatocellular transplantation. Here, we investigated the biological characteristics of USCs and their potential use for the ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：OBJECTIVE:Our overall goal was to investigate the anti-tumor activity of the glutaminase 1 (GLS1) Inhibitor compound 968 in ovarian cancer cells. The human ovarian cancer cell lines, HEY, SKOV3 and IGROV-1 were used. Cell proliferation was assessed by MTT assay after treatment with compound 968. Cell cycle progression ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-10-15 00:00:00
abstract：:We recently reported a novel anticancer small molecule, designated FL118, which was discovered via high throughput screening (HTS), and followed by hit-lead in vitro and in vivo analysis. FL118 selectively inhibits the expression of four major cancer survival-associated gene products (survivin, Mcl-1, XIAP, and cIAP2)...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Recent studies suggest that inhibiting the protein myostatin, a negative regulator of skeletal muscle mass, may improve outcomes in patients with Duchenne muscular dystrophy by enhancing muscle mass. When the dystrophin-deficient golden retriever muscular dystrophy (GRMD) dog was bred with whippets having a heterozygo...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
abstract：:Circular RNA hsa_circ_0002024 has been reported to be underexpressed in bladder cancer (BC). However, the biological role of hsa_circ_0002024 in BC and its underlying molecular mechanisms remain unclear. In this study, the expression levels of hsa_circ_0002024 and miR-197-3p were examined by quantitative real-time pol...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:Thymopoiesis is essential and significant for development and maintenance of the robust and healthy immune system. The acute suppression of thymopoiesis induced by 5-Azacytidine (5-Aza) is an intractable clinical problem complicating chemotherapy. Interleukin 1 receptor antagonist (IL-1Ra) is a cytokine that competiti...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
abstract：:Although microRNAs (miRNAs) play an important role in esophageal squamous cell carcinoma (ESCC), their roles in radiotherapy response remain unexplored. Our study aims to investigate whether plasma miRNAs can be used as predictors of radiotherapy outcome in ESCC patients. We selected nine miRNAs, which were reported t...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
abstract：:Isoflurane (ISO) exhibits neuroprotective effects against inflammation and apoptosis. However, the role of ISO in motoneuronal apoptosis induced by activated microglia remains poorly studied. We investigated the protective effects of ISO on the apoptosis of ventral spinal cord 4.1 (VSC4.1) motoneurons induced by lipop...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-03-15 00:00:00
abstract：BACKGROUND:We previously constructed AdEasy system for rapid generation of recombinant adenovirus expressing coding genes. However, it is unclear if AdEasy system could be used for exogenously expression of long noncoding RNAs (lncRNAs). Here we investigated how to overexpress lncRNA H19 with AdEasy system and identifi...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-05-15 00:00:00
abstract：:Background/Introduction: Aberrant expression of Toll like receptors (TLR) plays a vital role in pathogenesis of rheumatoid arthritis (RA). Micro RNAs (miRs) could play important role in the related signaling pathways. The present study was undertaken to establish the link between miR-147 and TLR-7 in rat macrophages (...
journal_title：American journal of translational research
pub_type： 杂志文章,收录出版
doi：
更新日期：2019-04-15 00:00:00
abstract：:Immunotherapy using antibodies blocking the programmed cell death 1 (PD-1)/programmed cell death-ligand 1 (PD-L1) pathway has achieved great success in preclinical models and the clinical treatment of esophageal squamous cell carcinoma (ESCC). The c-Myc proto-oncogene helps prevent immune cells from attacking tumor ce...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-02-15 00:00:00
abstract：:Extracorporeal pulsed electromagnetic field (PEMF) has been shown the ability to improve regeneration in various ischemic episodes. Here, we examined whether PEMF therapy facilitate cardiac recovery in rat myocardial infarction (MI), and the cellular/molecular mechanisms underlying PEMF-related therapy was further inv...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2014-05-15 00:00:00
