Abstract：
:The abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are crucial pathological processes that are involved in atherosclerosis. Growing evidence suggests that microRNAs (miRNAs) play critical roles in VSMCs functions. Here, we analyzed the expression of four atherosclerosis-related miRNAs and found that hsa-miR-148b was significantly down-regulated in plaques from atherosclerotic patients compared to a healthy control group. The restoration of hsa-miR-148b function in cells transfected with a hsa-miR-148b mimicmarkedly inhibited VSMCs proliferation and migration compared to a hsa-miR-148b mimic control. Furthermore, we discovered that heat shock protein 90 (HSP90) was a direct target of hsa-miR-148b in VSMCs. Hsa-miR-148b suppressed HSP90 expression by directly binding its 3'-untranslated region (UTR). In addition, the expression of hsa-miR-148b was negatively correlated with the HSP90 mRNA levels in plaques of atherosclerotic patients. Interestingly, the overexpression of HSP90 partly abrogated the hsa-miR-148b-mediated inhibition of VSMCs proliferation and migration. Our study provides the first evidence that hsa-miR-148b has anti-proliferative and migratory functions by targeting HSP90 in VSMCs and may aidin the development of new biomarkers and potential therapeutic targets for atherosclerosis.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Zhang X,Shi H,Wang Y,Hu J,Sun Z,Xu Ssubject
Has Abstractpub_date
2017-02-15 00:00:00pages
629-637issue
2issn
1943-8141journal_volume
9pub_type
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2019-10-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2019-05-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2018-12-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2018-03-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2018-09-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2020-10-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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更新日期：2020-11-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2015-04-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2017-03-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
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更新日期：2009-05-08 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2020-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-02-15 00:00:00
abstract：BACKGROUND:We previously constructed AdEasy system for rapid generation of recombinant adenovirus expressing coding genes. However, it is unclear if AdEasy system could be used for exogenously expression of long noncoding RNAs (lncRNAs). Here we investigated how to overexpress lncRNA H19 with AdEasy system and identifi...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-05-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-12-15 00:00:00
abstract：:Tetraspanin 12 (TSPAN12), as an earliest member of tetraspanin family, has been recently shown to be highly expressed in several malignant tumors, such as lung cancer and breast cancer, which plays an important role in regulating cell proliferation, migration and invasion. However, the functional roles of TSPAN12 in c...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-02-15 00:00:00
abstract：:Myoblasts characterize by the potential to transform into skeletal muscle, and involve in processes of proliferation, differentiation and apoptosis. Mammalian target of rapamycin (mTOR) is an important protein of PI3K signaling pathway in muscle metabolism and physiology. This study aimed to investigate effects of mTO...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:Enoxaparin is used postoperatively for the prevention of venous thromboembolism. In vitro studies and clinical trials have demonstrated the anticoagulant and antithrombotic efficacy of enoxaparin. In this study, we visualised thromboprophylactic and thrombolytic efficacy of enoxaparin in a laser-induced thrombus forma...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-01-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2009-07-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-06-15 00:00:00
abstract：BACKGROUND:To provide novel insight into the development of new therapeutic strategies to combat breast cancer, differentiation-inducing activity of clinically achievable concentrations of arsenite (AsIII) and tetrandrine (Tetra) was investigated in breast cancer cell lines MDA-MB-231 and MCF-7. METHODS:Differentiatio...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-12-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-05-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-09-15 00:00:00
abstract：:Objectives: Persistent low-grade chronic inflammation is common in type 2 diabetes (T2D) and obesity. To date, the underlying molecular mechanism is not well understood. In this study, we aimed to investigate gut microbiota and the expression of monocyte surface molecules in obese T2D subjects who underwent Roux-en-Y ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-03-15 00:00:00
