Abstract：
:Severe acute pancreatitis (SAP) contributes to multiple organ dysfunction and intestine is one of the most susceptible targets. This study aims to explore the role of C3a/C3aR axis in SAP-induced intestinal barrier injury. Adult male Sprague Dawley rats were randomly divided into control, SAP, C3aRA (0.06 mg/kg) and C3aRA (0.12 mg/kg) groups. SAP rat models were established by retrograde injection of 3.5% sodium taurocholate solutions into pancreatic ducts. Histopathological changes and dysfunction in pancreatitis and intestine were measured by hematoxylin and eosin (H&E) staining and detection of amylase (AMY), lipase (LIPA), endotoxins and diamine oxidase (DAO) levels in serum. Cell apoptosis was evaluated by TUNEL assay and western blot analysis. In addition, the expressions of caudin-1, caudin-2, occludin and ZO-1 were detected by western blot assay and immunohistochemical staining. Inflammatory cytokines and oxidative stress levels in SAP rats were determined. The C3a/C3aR expression was increased in pancreatic and intestinal tissues of successfully established SAP rat models. C3a receptor antagonist (C3aRA) alleviated pancreatic and intestinal pathological lesions and dysfunction induced by SAP. C3aRA inhibited cell apoptosis and promoted the expressions of caudin-1, caudin-2, occludin and ZO-1 in intestinal tissues. Moreover, C3aRA repressed inflammatory cytokines by reduction of TNF-α, IL-1β, IL-6 and MCP-1 levels, and ameliorated oxidative stress through regulation of ROS, MPO and SOD activity in rats with SAP-induced intestinal barrier injury. Our findings suggested that inhibition of C3a/C3aR axis diminished pancreatic damage and SAP-induced intestinal barrier injury in vivo, which may provide a new therapeutic strategy for SAP-induced intestinal injury.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Ye J,Dai H,Liu Y,Yu B,Yang J,Fei Asubject
Has Abstractpub_date
2020-10-15 00:00:00pages
6290-6301issue
10issn
1943-8141journal_volume
12pub_type
杂志文章abstract：:An ideal implantation of artificial cervical disc replacement (ACDR) prosthesis should preserve the cervical biomechanics and maintain normal cervical biomechanics. In this study, we designed a novel prosthesis based on the physiological curvature of the cervical endplate, and conducted an in vitro test with cadaveric...
journal_title：American journal of translational research
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更新日期：2019-05-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2019-07-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2016-11-15 00:00:00
abstract：BACKGROUND:Emerging evidence indicated that dysregulated long non-coding RNAs (lncRNAs) was implicated in the tumorigenesis and progression. LncRNA TUSC7 was involved in various malignancies. However, the role of TUSC7 in human non-small-cell lung cancer (NSCLC) remains unclear. METHODS:Expression of TUSC7 was analyze...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-02-15 00:00:00
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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journal_title：American journal of translational research
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更新日期：2019-08-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2020-09-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2017-11-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:Urine-derived stem cells (USCs) are autologous stem cells that exhibit self-renewal ability and multi-lineage differentiation potential. These characteristics make USCs an ideal cell source for hepatocellular transplantation. Here, we investigated the biological characteristics of USCs and their potential use for the ...
journal_title：American journal of translational research
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doi：
更新日期：2020-09-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2020-11-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-07-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-10-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-05-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-06-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-11-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章,评审
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更新日期：2019-02-15 00:00:00
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journal_title：American journal of translational research
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doi：
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journal_title：American journal of translational research
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doi：
更新日期：2020-07-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2017-08-15 00:00:00
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journal_title：American journal of translational research
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更新日期：2017-03-15 00:00:00
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journal_title：American journal of translational research
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doi：
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journal_title：American journal of translational research
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更新日期：2020-03-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
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journal_title：American journal of translational research
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更新日期：2017-03-15 00:00:00
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journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-10-15 00:00:00
