Abstract:
:Random pattern skin flap transplantation is frequently applied in plastic and reconstructive surgery, but the distal part of skin flaps often suffers necrosis due to ischemia. Astragaloside IV (AS-IV), a natural saponin purified from Astragalus membranaceus, may have beneficial functions for flap survival. In this study, rats were divided into a control group and an AS-IV treatment group, and underwent surgery using a modified "McFarlane flap" model. After intragastric administration of vehicle control or AS-IV for their respective groups, flap survival area and water content were measured 7 days after surgery. Flap tissue was separated to test protein expressions related to angiogenesis, inflammation, oxidative stress and autophagy via western blot, immunohistochemistry, and immunofluorescence. Results showed that AS-IV improved flap survival area and reduced tissue edema. AS-IV also increased mean vessel densities and upregulated levels of VEGF protein, both of which indicate increased angiogenesis. Furthermore, AS-IV depressed leukocyte infiltration, decreased expressions of inflammatory proteins TNF-α, IL1β and IL6, increased SOD activity, decreased MDA content, and stimulated autophagy. Overall, our results suggest that AS-IV promotes skin flap survival via inducing angiogenesis, depressing inflammation and dampening oxidative stress; it also activates autophagy, which may be an underlying mechanism for oxidative stress depression.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Lin R,Chen H,Callow D,Li S,Wang L,Li S,Chen L,Ding J,Gao W,Xu H,Kong J,Zhou Ksubject
Has Abstractpub_date
2017-09-15 00:00:00pages
4161-4172issue
9issn
1943-8141journal_volume
9pub_type
杂志文章abstract::The objective of this paper was to investigate the suitability of the miniature pig as a large animal model of cochlear implantation (CI). Micro-CT scanning and three-dimensional reconstructions of the inner ear were completed in six animals. Photographs of the procedures and measurements of the inner ear were made. T...
journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2016-12-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2015-11-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2015-12-15 00:00:00
abstract:OBJECTIVE:To investigate the effects of paeoniflorin, the main monomer component of Jinxueyuan granules, on the Ca2+ concentrations in salivary gland cells to further explore the salivation-promoting mechanism and effective monomer components of Jinxueyuan granules. METHODS:The salivary gland cells of suckling rats we...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most common and invasive tumor of the central nervous system. Growth factors and cytokines (GFCKs) play a crucial role in tumor invasion. In the present study, GFCK expression profiles from GBM patients in the Chinese Glioma Genome Atlas were used to perform sample clustering with ...
journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2020-10-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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doi:
更新日期:2016-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-01-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2019-11-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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doi:
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2016-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2019-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2018-08-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2018-04-15 00:00:00