Abstract:
:Urine-derived stem cells (USCs) are autologous stem cells that exhibit self-renewal ability and multi-lineage differentiation potential. These characteristics make USCs an ideal cell source for hepatocellular transplantation. Here, we investigated the biological characteristics of USCs and their potential use for the treatment of chronic liver injury. We characterized the cell-surface marker profile of USCs by flow cytometry and determined the osteogenic, adipogenic, and hepatic differentiation capacities of USCs using histology. We established a chronic liver-injury model by intraperitoneally injecting carbon tetrachloride into nude mice. USCs were then transplanted via tail vein injection. To determine liver function and histopathology following chronic liver injury, we calculated the liver index, measured serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and performed histological staining. USCs were small, adherent cells expressing mesenchymal but not hematopoietic stem-cell markers. Some induced USCs underwent osteogenic and adipogenic differentiation. When co-cultured with hepatic progenitor cells, about 10% of USCs underwent hepatic differentiation. The ALT and AST levels of the USC-transplanted group were lower than that of the chronic liver-injury model group, and there were no significant differences between the two USC-transplanted groups. However, hepatocyte degeneration and liver fibrosis substantially improved in the hypoxia-pretreated USC-transplanted group compared with the normoxia USC-transplanted group. Taken together, USCs display desirable proliferation and differentiation characteristics, and USC transplantation partially improves abnormal liver function and pathology associated with chronic liver injury. Furthermore, hypoxia pretreatment promotes cell proliferation, migration, and colony formation by inducing autophagy, leading to USC-elicited liver tissue recovery following injury in vivo.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Hu C,He Y,Fang S,Tian N,Gong M,Xu X,Zhao L,Wang Y,He T,Zhang Y,Bi Ysubject
Has Abstractpub_date
2020-09-15 00:00:00pages
5131-5150issue
9issn
1943-8141journal_volume
12pub_type
杂志文章abstract::miR-101 is significantly downregulated in various human cancers, including oral squamous cell carcinoma (OSCC). However, the role of miR-101 in OSCC has not been elucidated. In this study, miR-101 lowly expressed in OSCC tissues and cell lines compared with that in adjacent normal tissues and human normal oral keratin...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract::Extinction bursts are characterized by a temporary increase in responding when drug access is withheld from rats trained to self-administer drugs of abuse. Thus far, one study has examined extinction bursts for nicotine self-administration using a 23-h access paradigm [1]. Here we examined extinction bursts using prev...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::Interleukin-1β (IL-1β) plays an important role in brain injury after focal ischemia, and bone marrow-derived mesenchymal stem cells (BMSCs) are capable of reducing the expression of IL-1β, we investigated the effects of BMSCs transplantation on brain edema and cerebral infarction as well as the underlying mechanisms v...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-03-15 00:00:00
abstract:BACKGROUND:Cyclosporin A (CsA) and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). NFAT compose a family of transcription factors that are turned on during ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2009-01-22 00:00:00
abstract:BACKGROUND:Systemic maternal inflammation and neonatal hyperoxia arrest alveolarization in neonates. The aims were to test whether human mesenchymal stem cells (MSCs) reduce lung inflammation and improve lung development in perinatal inflammation- and hyperoxia-induced experimental bronchopulmonary dysplasia. METHODS:...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
abstract::Fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary flavonoid and has been indicated as a novel anti-cancer agent in several types of cancer cells. However, the mechanisms underlying the effect of fisetin in human oral squamous cell carcinoma (OSCC) remain unclear. Here, we report that fisetin significantly inhibits ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-12-15 00:00:00
abstract::Research use only (RUO) assays do not undergo a validation process similar to test kits used for clinical purposes. Several studies have suggested that RUO assays need to be validated prior to use in any research studies. We evaluated a research use only Luminex platform based assay for measuring serum procalcitonin l...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
abstract:OBJECTIVE:Our overall goal was to investigate the anti-tumor activity of the glutaminase 1 (GLS1) Inhibitor compound 968 in ovarian cancer cells. The human ovarian cancer cell lines, HEY, SKOV3 and IGROV-1 were used. Cell proliferation was assessed by MTT assay after treatment with compound 968. Cell cycle progression ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
abstract::Biceps tendinopathy is a relatively common ailment that typically presents as pain, tenderness, and weakness in the tendon of the long head of the biceps brachii. Though it is often associated with degenerative processes of the rotator cuff and the joint, this is not always the case, thus, the etiology remains conside...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2017-06-15 00:00:00
abstract:BACKGROUND:Mesenchymal stem cell (MSC)-based bone tissue engineering is a promising treatment option for maxillary sinus augmentation. Rapid vascularization is necessary to enhance the osteoinductive efficacy and prevent necrosis of the tissue-engineered bone. This study investigated whether the co-autotransplantation ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-06-15 00:00:00
abstract:BACKGROUND:Colorectal cancer is prevalent worldwide and it is also the fourth most common cause of cancer mortality. For rectal cancer, neoadjuvant concurrent chemoradiotherapy (CCRT) followed by radical proctectomy is gold standard treatment for patients with stage II/III rectal cancer. By data mining a public dataset...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
abstract::Isoflurane (ISO) exhibits neuroprotective effects against inflammation and apoptosis. However, the role of ISO in motoneuronal apoptosis induced by activated microglia remains poorly studied. We investigated the protective effects of ISO on the apoptosis of ventral spinal cord 4.1 (VSC4.1) motoneurons induced by lipop...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-03-15 00:00:00
abstract::Type 2 diabetes mellitus (T2DM), a leading cause of osteoporosis, remains a contraindication for bone implant therapy. Although associated with side effects when systemically administered, biphosphonates (BPs) play a positive role in diabetic osteoporosis treatment. We hypothesized that local BP therapy would prevent ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-09-15 00:00:00
abstract::Ankylosing spondylitis (AS) is a type of rheumatic inflammatory disease. miRNAs participate in the process of regulating inflammatory response and bone differentiation. Herein, we aimed to test the effect of miR-495 on AS. The serum and tissues were obtained from traumatic fracture (health) and AS patients. The human ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
abstract::Gastric cancer (GC) causes nearly one million deaths worldwide each year. However, the molecular pathway of GC development remains unclear. Increasing evidences have shown that microRNAs (miRNAs) are highly associated with tumor development. However, relative little is known about the potential role of miRNAs in gastr...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
abstract::We investigated the molecular mechanisms involved in transforming growth factor beta 1 (TGF-β1)-induced myogenic stem cell differentiation to smooth muscle cells. We isolated muscle-derived stem cells (MDSCs) from gastrocnemius muscles following their identification by immunohistochemistry analysis of desmin and flow ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-08-15 00:00:00
abstract::Erythema elevatum diutinum (EED) is a rare chronic inflammatory dermatosis and a part of the spectrum of cutaneous leukocytoclasticvasculitis. The most common site of involvement is extensor surface of the extremities, with a predilection for the skin overlying joints, particularly hands, feet, elbows and knees, as we...
journal_title:American journal of translational research
pub_type:
doi:
更新日期:2017-04-15 00:00:00
abstract:BACKGROUND:Gastric cancer (GC) is one of the most common digestive tract tumors, and a serious threat to human health. Long non-coding RNA (lncRNA) are involved in many cancers. However, the biological functions of most lncRNAs are unclear. In this study, we investigated the mechanisms by which FLVCR1-AS1 regulated GC ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::The current study aims to measure distance parameters in lumbar isthmus to develop new references for lumbar pedicle screw insertion. Using computed radiography, the distance between the median pedicle tangent and the isthmus lateral tangent (D1) and the isometric distance between the isthmus lateral tangent and the i...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
abstract::Breast cancer, a worldwide disease with increasing incidence, develops from ductal/lobular epithelium. Nipple aspirate fluid (NAF), secreted from the breast ducts and lobules, can be analyzed to assess metabolic activity in breast microenvironment. Premalignant and malignant cell alterations may produce biochemical si...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2010-10-30 00:00:00
abstract::Angiopoietin-1 (Ang-1) plays a critical role in the regulation of endothelial cell survival and vascular maturation and stability. However, its role in hematopoiesis is not clear. Here, we determined effect of Ang-1 on the recovery of hematopoiesis in radiated mice. By injecting an Ang-1 plasmid, we found that Ang-1 w...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-05-15 00:00:00
abstract::Incidence of nasopharyngeal carcinoma (NPC) has remained high worldwide, posing a serious health problem. MicroRNAs (miRNAs) are a family of about 20-23 nucleotides small non-coding molecules, which play a significant role in NPC. In this study, we explored the molecular mechanisms of miR-130a-3p in inhibiting viabili...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-08-15 00:00:00
abstract::Endothelial barrier dysfunction is a critical pathophysiological process of pulmonary ischemia/reperfusion (I/R) injury in patients scheduled for cardiopulmonary bypass. Impaired actin cytoskeleton dynamics and cell-cell junctions are the main causes of endothelial dysfunction. Statins have protective effects on I/R-i...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
abstract::Cyclooxygenase-2 expression by malignant tumors, including colonic adenocarcinoma, is associated with increased tumor aggression and poor prognosis. Nuclear factor kappa B is a key regulator of cyclooxygenase-2 and is regulated by two pathways, the 'canonical' and the 'alternative' pathway. The alternative pathway is ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-09-15 00:00:00
abstract::Targeted oncolytic adenoviruses can selectively replicate in cancer cells; combined with traditional chemotherapy drugs, this approach is expected to become an important treatment method for overcoming the current bottleneck of osteosarcoma treatment. Here, we investigate the effect of oncolytic adenovirus Ad11 combin...
journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2020-01-15 00:00:00
abstract::This study aimed to determine applicable value of DWIBS in diagnosis of solitary pulmonary lesions. This study involved 32 solitary lung disease patients. T1W1, T2W1, T2WI-SPAIR were examined using MRI scanner and analyzed with View-forum 6.0 workstation. Imaging characteristics of pulmonary solitary lesions on DWIBS ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2021-01-15 00:00:00
abstract::Immune regulatory system dysfunction plays a role in the pathogenesis of asthma. The therapeutic effect of allergic asthma is to be improved. The immune regulatory function of probiotics has been recognized. This study tests a hypothesis that Clostridium butyricum (CB) enhances the effect of allergen specific immunoth...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-12-15 00:00:00
abstract::Both POEMS syndrome and IgG4 related diseases are uncommon and clinical manifestations have been multifarious and nonspecific. There is still no literature describing the relationships between these two diseases. This study describes a case of a 33-year-old woman with POEMS syndrome, who had high serum IgG4 concentrat...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
abstract::Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase which utilizes collagen as a ligand to regulate the interaction between cancer cells and tumor stroma. However, the clinical relevance of DDR1 expression in bladder cancer as well as its molecular regulation have not been previously investigated. Here, w...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-05-15 00:00:00
abstract::Due to practical and ethical concerns associated with human experimentation, animal models have been essential in cancer research. However, the average rate of successful translation from animal models to clinical cancer trials is less than 8%. Animal models are limited in their ability to mimic the extremely complex ...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-15 00:00:00