Abstract:
:We investigated the molecular mechanisms involved in transforming growth factor beta 1 (TGF-β1)-induced myogenic stem cell differentiation to smooth muscle cells. We isolated muscle-derived stem cells (MDSCs) from gastrocnemius muscles following their identification by immunohistochemistry analysis of desmin and flow cytometry analysis of SCA-1, CD34, and CD45. MDSCs at passage 3 (PP3) were cultured in vitro to examine the effects of MDSC induction. Gene ontology and KEGG pathway analyses were performed to analyze these differentially expressed genes. Reduced representation bisulfite sequencing was performed in TGF-β1-treated and untreated cells to evaluate differences in the methylation status and analyze the chromosomal distribution of differentially methylated sites (DMSs). Significant morphological changes to cells were observed at PP3, and most PP3 cells were positive for desmin and SCA-1, and were confirmed to be MDSCs. Results of western blot and immunohistochemistry analyses suggested that expressions of a-SMA and CNN1 significantly increased after treatment with TGF-β1. Global transcriptome analysis identified 1996 differentially expressed genes (MSC_TGFβ1/MSC_NC). Results of methylome analysis indicated that there were more hypermethylation sites in the untreated group than in the TGF-β1-treated group. Most DMSs were hypermethylated, whereas a small portion was hypomethylated. The chromosomal distribution of DMSs indicated that chromosome 1 had the highest proportion of DMSs, whereas the Y chromosome had the fewest DMSs. Sud2, Pcdh19, and Nat14 are potential core genes involved in cell differentiation. These results may explain the mechanisms of cell differentiation and provide useful information regarding diseases such as pelvic organ prolapse.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Tang X,Su X,Zhong Z,Wen C,Zhang T,Zhu Ysubject
Has Abstractpub_date
2019-08-15 00:00:00pages
5150-5161issue
8issn
1943-8141journal_volume
11pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2016-02-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
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更新日期:2020-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2019-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2011-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2014-10-11 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2020-11-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2020-12-15 00:00:00
abstract:AIMS:This study was to investigate the effect of TUG1 on apoptosis and ECM degradation of human degenerative intervertebral disc nucleus pulposus cells (NPCs) and its mechanism. METHODS:Human degenerative intervertebral disc NP tissues were obtained from 10 patients with lumbar disc herniation (LDH) who underwent lumb...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
abstract::The quantity and function of osteoblasts require continuous osteogenic differentiation of bone marrow mesenchymal stem cells. Recent evidence suggests that microRNAs (miRNAs) act as important post-transcriptional regulators in a wide range of biological processes, including osteoblastic differentiation. Quercetin has ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-05-15 00:00:00
abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant neoplasm originated from the nasopharyngeal epithelium and is mainly prevalent in Southern China and Southeast Asia countries. KiSS-1 is an inhibitor of tumor metastasis in a range of cancers. METHODS:We establish a cell substrain of SUNE-1-5-8...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::Liver cancer, one of the most common malignant tumors occurred worldwide, has emerged as a main health trouble and accounts for leading cancer-related death. Diosgenin is provided as an important material in the pharmaceutical industry, and is used to manage various medical troubles such as cancer because of its multi...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-11-15 00:00:00
abstract::Curcumin (Cur) has multiple pharmacological effects including antitumor, anti-inflammatory, antioxidant and cardiovascular protective effects. This research aims to further explore whether the cardiovascular protective effects of Cur are mediated by the miR-590-3p/CD40 pathway. Endothelial cells (ECs) were cultivated ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-02-15 00:00:00