Abstract:
BACKGROUND:Hepatitis C virus (HCV) Core protein regulates multiple signaling pathways and alters cellular genes expression responsible for HCV induced pathogenesis leading to hepatocellular carcinoma (HCC). Prevalence of HCV genotype 3a associated HCC is higher in Pakistan as compare to the rest of world; however the molecular mechanism behind this is still unclear. This study has been designed to evaluate the effect of HCV core 3a on apoptosis and cell proliferation which are involved in HCC METHODOLOGY: We examined the in vitro effect of HCV Core protein of genotype 3a and 1a on cellular genes involved in apoptosis by Real time PCR in liver cell line (Huh-7). We analyzed the effect of HCV core of genotype 1a and 3a on cell proliferation by MTT assay and on phosphrylation of Akt by western blotting in Huh-7 cells. RESULTS:The HCV 3a Core down regulates the gene expression of Caspases (3, 8, 9 and 10), Cyto C and p53 which are involved in apoptosis. Moreover, HCV 3a Core gene showed stronger effect in regulating protein level of p-Akt as compared to HCV 1a Core accompanied by enhanced cell proliferation in Huh-7 cell line. CONCLUSION:From the current study it has been concluded that reduced expression of cellular genes involved in apoptosis, increased p-Akt (cell survival gene) and enhanced cell proliferation in response to HCV 3a core confirms anti apoptotic effect of HCV 3a Core gene in Huh-7 that may lead to HCC.
journal_name
Virol Jjournal_title
Virology journalauthors
Jahan S,Khaliq S,Siddiqi MH,Ijaz B,Ahmad W,Ashfaq UA,Hassan Sdoi
10.1186/1743-422X-8-522subject
Has Abstractpub_date
2011-11-23 00:00:00pages
522issn
1743-422Xpii
1743-422X-8-522journal_volume
8pub_type
杂志文章相关文献
Virology Journal文献大全abstract::Bats have been recognized as the natural reservoirs of a large variety of viruses. Special attention has been paid to bat coronaviruses as the two emerging coronaviruses which have caused unexpected human disease outbreaks in the 21st century, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Re...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-015-0422-1
更新日期:2015-12-22 00:00:00
abstract::The original version of the acknowledgement unfortunately contained a mistake. ...
journal_title:Virology journal
pub_type: 已发布勘误
doi:10.1186/s12985-020-01439-2
更新日期:2020-11-09 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) is a major cause of chronic hepatitis and hepatocellular carcinoma (HCC) and different HCV genotypes show characteristic variations in their pathological properties. Insulin resistance (IR) occurs early in HCV infection and may synergize with viral hepatitis in HCC development. Egypt ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-496
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Farmington virus (FARV) is a rhabdovirus that was isolated from a wild bird during an outbreak of epizootic eastern equine encephalitis on a pheasant farm in Connecticut, USA. FINDINGS:Analysis of the nearly complete genome sequence of the prototype CT AN 114 strain indicates that it encodes the five canoni...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-219
更新日期:2013-07-01 00:00:00
abstract::Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cro...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-482
更新日期:2011-10-27 00:00:00
abstract:BACKGROUND:Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it. METHOD:In this study, we attempted to eluci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0654-8
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Chronic hepatitis C virus (HCV) genotype (GT) 3 infection with advanced liver disease has emerged as a challenging to treat by direct-acting antivirals (DAAs), but the efficacy of DAAs in Chinese HCV-GT3 patients is rarely reported. This study aimed to analyze the efficacy of sofosbuvir (SOF)-based regimens ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1066-8
更新日期:2018-10-01 00:00:00
abstract::The I5L gene is one of approximately 90 genes that are conserved throughout the chordopoxvirus family, and hence are presumed to play vital roles in the poxvirus life cycle. Previous work had indicated that the VP13 protein, a component of the virion membrane, was encoded by the I5L gene, but no additional studies had...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-148
更新日期:2008-12-15 00:00:00
abstract:BACKGROUND:Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian tiger mosquitoes. To study the processing of envelope proteins E1 and...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-353
更新日期:2011-07-15 00:00:00
abstract::Phage vB_EcoM_CBA120 (CBA120), isolated against Escherichia coli O157:H7 from a cattle feedlot, is morphologically very similar to the classic phage ViI of Salmonella enterica serovar Typhi. Until recently, little was known genetically or physiologically about the ViI-like phages, and none targeting E. coli have been ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-430
更新日期:2011-09-07 00:00:00
abstract:BACKGROUND:Porcine epidemic diarrhea virus (PEDV) is the main causative agent of porcine epidemic diarrhea (PED). Since December 2010, a large-scale outbreak of diarrhea has been observed in swine farms in China. Accumulated evidence indicates that this large-scale outbreak of diarrhea were caused by highly virulent PE...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0529-z
更新日期:2016-04-22 00:00:00
abstract:BACKGROUND:Respiratory syncytial virus (RSV) is the leading viral pathogen associated with bronchiolitis and lower respiratory tract disease in infants and young children worldwide. The respiratory epithelium is the primary initiator of pulmonary inflammation in RSV infections, which cause significant perturbations of ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-114
更新日期:2008-10-06 00:00:00
abstract:BACKGROUND:Cell culture adaptation of very virulent infectious bursal disease virus (vvIBDV) was shown to be mainly associated with the VP2 capsid protein residues 253, 279, and 284. The single mutation A284T proved critical for cell culture tropism, but did not confer efficient virus replication, which at least requir...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-014-0199-7
更新日期:2014-11-25 00:00:00
abstract:BACKGROUND:HIV-1 is highly variable genetically and at protein level, a property it uses to subvert antiviral immunity and treatment. The aim of this study was to assess if HIV subtype differences were associated with variations in glycosylation patterns and co-receptor tropism among HAART patients experiencing differe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0703-y
更新日期:2017-02-14 00:00:00
abstract:BACKGROUND:Airborne viruses remain one of the major public health issues worldwide. Detection and quantification of airborne viruses is essential in order to provide information regarding public health risk assessment. FINDINGS:In this study, an optimized new, simple, low cost method for sampling of airborne viruses u...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-369
更新日期:2011-07-27 00:00:00
abstract:BACKGROUND:The aquatic birnavirus infectious pancreatic necrosis virus (IPNV) causes infectious pancreatic necrosis (IPN), a severe disease in farmed salmonid fish. IPNV has a very broad host range and infects many different species of fish as well as molluscs and crustaceans. Investigation of the host reservoir of a v...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-228
更新日期:2012-10-05 00:00:00
abstract:BACKGROUND:Few studies have used quantitative polymerase chain reaction (qPCR) as an approach to measure virus neutralization assay endpoints. Its lack of use may not be surprising considering that sample nucleic acid extraction and purification can be expensive, labor-intensive, and rate-limiting. METHODS:Virus/antib...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-195
更新日期:2013-06-15 00:00:00
abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01353-7
更新日期:2020-06-22 00:00:00
abstract:BACKGROUND:Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have mad...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1001-z
更新日期:2018-05-25 00:00:00
abstract:BACKGROUND:Pseudorabies virus (PRV) of the family Herpesviridae is the causative agent of Aujeszky's disease. Attenuation of PRV by serial passaging in vitro is a well-established method; however, the dynamic variations occurring on viral genome during this process have not been characterized. METHODS:Genome sequencin...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1102-8
更新日期:2018-12-29 00:00:00
abstract:BACKGROUND:To assess the potential effects of telbivudine (LdT) and entecavir (ETV) on renal function in patients with chronic hepatitis B (CHB), we performed a meta-analysis of the relevant data available on these agents to evaluate their effects on the estimated glomerular filtration rate (eGFR) during treatment. ME...
journal_title:Virology journal
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12985-016-0522-6
更新日期:2016-04-09 00:00:00
abstract:BACKGROUND:Dengue viruses (DENV) are the most important arboviruses of humans and cause significant disease. Infection with DENV elicits antibody responses to the envelope glycoprotein, predominantly against immunodominant, cross-reactive, weakly-neutralizing epitopes. These weakly-neutralizing antibodies are implicate...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-115
更新日期:2012-06-18 00:00:00
abstract:BACKGROUND:Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain (192-BJ07), which was...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-29
更新日期:2010-02-08 00:00:00
abstract:BACKGROUND:Oral malignancy is a major global health problem. Besides the main risk factors of tobacco, smoking and alcohol, infection by human papillomavirus (HPV) and genetic alterations are likely to play an important role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-253
更新日期:2010-09-23 00:00:00
abstract::From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool con...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-174
更新日期:2010-07-28 00:00:00
abstract:BACKGROUND:Infection with Chikungunya alphavirus (CHIKV) can cause severe arthralgia and chronic arthritis in humans with persistence of the virus in perivascular macrophages of the synovial membrane by mechanisms largely ill-characterized. FINDINGS:We herein analysed the innate immune response (cytokine and programme...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-313
更新日期:2012-12-19 00:00:00
abstract:BACKGROUND:To explore the relationship between enteroviruses and hospitalized children with hand, foot and mouth disease (HFMD) complicated with nervous system disease. 234 hospitalized HFMD patients treated in Shengjing Hospital, Liaoning Province were analyzed retrospectively. Based on the presence and severity of ne...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-8
更新日期:2012-01-09 00:00:00
abstract:BACKGROUND:Rhabdoviridae is one of the most ecologically diverse families of RNA viruses which can infect a wide range of vertebrates and invertebrates. Bats, among mammals, are pointed to harbor a significantly higher proportion of unknown or emerging viruses with zoonotic potential. Herein, we report the isolation of...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-0949-z
更新日期:2018-02-17 00:00:00
abstract:BACKGROUND:The influenza RNA dependent RNA polymerase synthesizes viral RNA in the nucleus as functional viral ribonucleoprotein (vRNP) complexes with RNA and nucleoprotein (NP). The N-terminus of NP contains an unconventional nuclear localization signal (NLS) important for initial vRNP nuclear localization but which a...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-155
更新日期:2014-08-31 00:00:00
abstract:BACKGROUND:It is becoming progressively more understandable that genetic variability of viruses is a major challenge in translating the laboratory findings to clinic. Genetic variability is the underlying cause of variant viral proteins which are not targetable by host immunological machinery. METHODS:500 patients wer...
journal_title:Virology journal
pub_type: 临床试验,杂志文章
doi:10.1186/1743-422X-11-171
更新日期:2014-09-30 00:00:00