Abstract:
BACKGROUND:T4 phage is a model species that has contributed broadly to our understanding of molecular biology. T4 DNA replication and packaging share various mechanisms with human double-stranded DNA viruses such as herpes virus. The literature indicates that T4-like phage genomes have permuted terminal sequences, and are generated by a DNA terminase in a sequence-independent manner; METHODS:genomic DNA of T4-like bacteriophage IME08 was subjected to high throughput sequencing, and the read sequences with extraordinarily high occurrences were analyzed; RESULTS:we demonstrate that both the 5' and 3' termini of the IME08 genome starts with base G or A. The presence of a consensus sequence TTGGA|G around the breakpoint of the high frequency read sequences suggests that the terminase cuts the branched pre-genome in a sequence-preferred manner. Our analysis also shows that terminal cleavage is asymmetric, with one end cut at a consensus sequence, and the other end generated randomly. The sequence-preferred cleavage may produce sticky-ends, but with each end being packaged with different efficiencies; CONCLUSIONS:this study illustrates how high throughput sequencing can be used to probe replication and packaging mechanisms in bacteriophages and/or viruses.
journal_name
Virol Jjournal_title
Virology journalauthors
Jiang X,Jiang H,Li C,Wang S,Mi Z,An X,Chen J,Tong Ydoi
10.1186/1743-422X-8-194subject
Has Abstractpub_date
2011-04-27 00:00:00pages
194issn
1743-422Xpii
1743-422X-8-194journal_volume
8pub_type
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