Abstract:
Introduction:Cannabidiol (CBD) is reported to produce pain relief, but the clinically relevant cellular and molecular mechanisms remain uncertain. The TRPV1 receptor integrates noxious stimuli and plays a key role in pain signaling. Hence, we conducted in vitro studies, to elucidate the efficacy and mechanisms of CBD for inhibiting neuronal hypersensitivity in cultured rat sensory neurons, following activation of TRPV1. Methods:Adult rat dorsal root ganglion (DRG) neurons were cultured and supplemented with the neurotrophic factors NGF and GDNF, in an established model of neuronal hypersensitivity. Neurons were stimulated with CBD (Adven 150, EMMAC Life Sciences) at 1, 10, 100 nMol/L and 1, 10 and 50 µMol/L, 48 h after plating. In separate experiments, DRG neurons were also stimulated with capsaicin with or without CBD (1 nMol/L to10 µMol/L), in a functional calcium imaging assay. The effects of the adenylyl cyclase activator forskolin and the calcineurin inhibitor cyclosporin were determined. We also measured forskolin-stimulated cAMP levels, without and after treatment with CBD, using a homogenous time-resolved fluorescence (HTRF) assay. The results were analysed using Mann-Whitney test. Results:DRG neurons treated with 10 and 50 µMol/L CBD showed calcium influx, but not at lower doses. Neurons treated with capsaicin demonstrated robust calcium influx, which was dose-dependently reduced in the presence of low dose CBD (IC50 = 100 nMol/L). The inhibition or desensitization by CBD was reversed in the presence of forskolin and cyclosporin. Forskolin-stimulated cAMP levels were significantly reduced in CBD treated neurons. Conclusion:CBD at low doses corresponding to plasma concentrations observed physiologically inhibits or desensitizes neuronal TRPV1 signalling by inhibiting the adenylyl cyclase - cAMP pathway, which is essential for maintaining TRPV1 phosphorylation and sensitization. CBD also facilitated calcineurin-mediated TRPV1 inhibition. These mechanisms may underlie nociceptor desensitization and the therapeutic effect of CBD in animal models and patients with acute and chronic pain.
journal_name
J Pain Resjournal_title
Journal of pain researchauthors
Anand U,Jones B,Korchev Y,Bloom SR,Pacchetti B,Anand P,Sodergren MHdoi
10.2147/JPR.S258433subject
Has Abstractpub_date
2020-09-11 00:00:00pages
2269-2278issn
1178-7090pii
258433journal_volume
13pub_type
杂志文章abstract:Purpose:Undertreatment of trauma-related pain is common in the pre-hospital and hospital settings owing to barriers to the use of traditional standard of care analgesics. Low-dose methoxyflurane is an inhaled non-opioid analgesic with a rapid onset of pain relief that is approved for emergency relief of moderate-to-sev...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S292521
更新日期:2021-01-20 00:00:00
abstract:Background:This study evaluates the reporting quality of randomized controlled trials (RCTs) on acupuncture use for the treatment of postherpetic neuralgia and explores related factors. Methods:The following six databases PubMed, Embase, Cochrane Library, VIP, CNKI, and SinoMed, were systematically searched from their...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S210471
更新日期:2019-07-29 00:00:00
abstract:Objective:To describe the rate and demographics of pain among Vietnamese people in 48 provinces and describe the impact of pain on individuals, levels of satisfaction with treatment results, and behavior of pain sufferers. Methods:The cross-sectional study was conducted in adults presenting to outpatient clinics throu...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S184713
更新日期:2019-02-25 00:00:00
abstract:Background:Femoral nerve block (FNB) has been considered as an excellent analgesic modality in total knee arthroplasty (TKA) pain control. However, relatively high concentration of ropivacaine could lead to quadriceps muscle weakness and increase the risk of postoperative falls. Objective:This double-blinded randomize...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S217283
更新日期:2019-12-17 00:00:00
abstract::Purpose: Thirty percent of patients with postherpetic neuralgia (PHN) receiving conservative treatment report unsatisfactory pain relief. Epidural steroid injections (ESIs) are commonly used as a therapeutic intervention in these patients. In this study, we aimed to determine if there are variables that predict the ef...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S190646
更新日期:2019-04-23 00:00:00
abstract::It is broadly accepted that children of all age groups including (preterm) neonates and young infants can perceive pain and that there is an absolute need to treat their pain safely and effectively. The approved treatment options for children, particularly (preterm) neonates and young infants, are very limited with on...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S195788
更新日期:2019-05-23 00:00:00
abstract:Background:The objective of this study was to compare the clinical and radiological outcomes between patients with or without axial symptoms (AS) and investigate the risk factors associated with AS by multivariate regression analysis in anterior cervical discectomy and fusion (ACDF). Materials and methods:The records ...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S172953
更新日期:2018-10-26 00:00:00
abstract:PURPOSE:The zona incerta (ZI) is below the ventral tier of the thalamus and has a strong influence selectively in higher-order thalamic relays. Although neuropathic pain has been suggested to result from reduced gamma-aminobutyric acid (GABA) and GABAergic signaling in the ZI, the mechanisms remain unclear. Here, the r...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S131104
更新日期:2017-05-11 00:00:00
abstract:Aim:Operant conditioning has long been believed to influence the pain experience through a psychological reward pathway. This study was formulated to test the hypothesis that pain sensitivity may be enhanced >3 months if a monetary reward works as a reinforcement. Methods:Forty healthy subjects volunteered to particip...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S175494
更新日期:2018-10-04 00:00:00
abstract:Purpose:To investigate the ameliorative effects of Vitellaria paradoxa (VP) nut extract for an anterior cruciate ligament transection with medial meniscectomy (ACLT+MMx)-induced osteoarthritis (OA) in high-fat diet (HFD)-induced obese rats. Methods:The rats were fed by HFD for 5 weeks before surgery-induced OA. Rats w...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S228766
更新日期:2020-01-30 00:00:00
abstract::Low back pain in patients with myofascial pain syndrome is characterized by painful active myofascial trigger points (ATPs) in muscles. This article reviews a novel, noninvasive modality that combines simultaneous imaging and treatment, thus taking advantage of the electrodermal information available from imaged ATPs ...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S47540
更新日期:2013-06-25 00:00:00
abstract:Purpose:Pregabalin is indicated for postherpetic neuralgia (PHN) in multiple countries, including China. This analysis compared pregabalin efficacy and safety in Chinese and international patients with PHN. Patients and methods:Data from Chinese and international randomized, double-blind, placebo-controlled trials wer...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S157856
更新日期:2018-09-03 00:00:00
abstract:INTRODUCTION:Although the majority of opioids in the US are prescribed by nonpain specialists, these providers often report inadequate training in chronic pain management and opioid prescribing. The extent of health care providers' knowledge of opioid prescribing for chronic pain has not been well established. The purp...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S98273
更新日期:2016-03-10 00:00:00
abstract:Purpose:To explore the role of cervical sagittal alignment in the occurrence of adjacent-level ossification development (ALOD) in patients who underwent anterior cervical discectomy fusion with self-locking stand-alone polyetheretherketone cage, and the relationship between cervical sagittal alignment and clinical outc...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S160472
更新日期:2018-07-20 00:00:00
abstract:OBJECTIVE:Chronic pelvic pain (CPP) is widely acknowledged as a common problem with significant consequences for those diagnosed with this condition. There is a lack of studies with good sample size that provide a comprehensive psychological profile of women presenting to specialist chronic pain clinics. Therefore, the...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S115065
更新日期:2016-11-16 00:00:00
abstract::Pain is highly prevalent, but frequently untreated or under-treated, and health care professionals are faced with a range of treatment challenges. Multimodal therapy is recommended and can be achieved using open combinations (ie, concomitant administration) of individual agents, fixed-dose combinations (FDCs), or mult...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S208082
更新日期:2019-09-04 00:00:00
abstract::Generalized joint hypermobility (GJH) is highly prevalent among patients diagnosed with chronic pain. When GJH is accompanied by pain in ≥4 joints over a period ≥3 months in the absence of other conditions that cause chronic pain, the hypermobility syndrome (HMS) may be diagnosed. In addition, GJH is also a clinical s...
journal_title:Journal of pain research
pub_type: 杂志文章,评审
doi:10.2147/JPR.S64251
更新日期:2015-08-20 00:00:00
abstract::Multisite pain, or pain that occurs simultaneously at >1 anatomical site, is more prevalent than single-site pain. While multisite pain affects over half of older adults, it remains an understudied pain entity that may have important functional implications in an aging population. Greater understanding of this complex...
journal_title:Journal of pain research
pub_type: 杂志文章,评审
doi:10.2147/JPR.S192755
更新日期:2019-03-29 00:00:00
abstract:Background:To date, there has been no study on the effects of interleukin-10 (IL-10) on complex regional pain syndrome (CRPS) rodent models, despite the anti-allodynic effect of IL-10 in previous studies. Thus, the aim of this study was to investigate the effect of IL-10 in a CRPS mouse model and find whether early inh...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S166624
更新日期:2018-09-04 00:00:00
abstract:Background:The ability to accurately diagnose and objectively localize pain generators in chronic pain sufferers remains a major clinical challenge since assessment relies on subjective patient complaints and relatively non-specific diagnostic tools. Developments in clinical molecular imaging, including advances in ima...
journal_title:Journal of pain research
pub_type:
doi:10.2147/JPR.S167839
更新日期:2018-10-12 00:00:00
abstract:Objective:To demonstrate the importance of recognizing and separating nonmedian nerve-related symptoms from those related to median nerve compression at the carpal tunnel. Methods:The records of 80 patients, aged 31-82 years (39 males and 41 females), who had undergone median nerve decompression using open and endosco...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S142153
更新日期:2017-10-24 00:00:00
abstract:OBJECTIVE:To evaluate the safety and efficacy of ketoprofen in Transfersome® gel (IDEA-033) in comparison with a ketoprofen-free vehicle (TDT 064) for the treatment of osteoarthritis (OA) of the knee. METHODS:Patients with knee OA (N = 866) were randomly assigned to receive topical IDEA-033 containing 100, 50, or 25 m...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S51054
更新日期:2013-10-25 00:00:00
abstract:OBJECTIVE:To characterize the steady-state pharmacokinetic profile of hydromorphone extended-release (ER) in patients with chronic pain taking concomitant medications. METHODS:This open-label repeat-dose study enrolled 22 patients (mean age, 51.4 years; 81.8% female). All patients were receiving at least one concomita...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S33807
更新日期:2012-01-01 00:00:00
abstract:Background:Low back pain is a prevalent symptom that occurs in all age of people, whereas the pathogenesis is unknown. Iliopsoas tendinopathy is an increasingly recognized hip disorder that may contribute to low back pain. Our purpose is to evaluate the effect of ultrasound-guided local injection of anesthetic and ster...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S281880
更新日期:2020-11-20 00:00:00
abstract:Background:Preoperative anxiety is associated with postoperative hyperalgesia; however, few studies have investigated the mechanism underlying this association in female surgical patients. Research has suggested that ON cells in the rostral ventromedial medulla (RVM) receive nerve impulses via cholecystokinin 2 (CCK2) ...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S187715
更新日期:2019-07-03 00:00:00
abstract:Purpose:Premonitory symptoms (PSs) of migraine are those that precede pain in a migraine attack. Previous studies suggest that treatment during this phase may prevent the onset of pain; however, this approach requires that patients be able to recognize their PSs. Our objectives were to evaluate patients' actual ability...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S175602
更新日期:2018-09-27 00:00:00
abstract:BACKGROUND:In Japan, there are limited options for switching opioid analgesics. Hydromorphone is an opioid analgesic that is routinely used instead of morphine for cancer pain; however, it is not yet available in Japan. The aim of this study was to assess the efficacy and safety of hydromorphone (DS-7113b) extended-rel...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S136937
更新日期:2017-08-18 00:00:00
abstract::Duloxetine is a serotonin-norepinephrine reuptake inhibitor approved by the US Food and Drug Administration for the treatment of fibromyalgia and painful diabetic neuropathy at doses of 60 mg daily. Duloxetine has been shown to significantly improve the symptoms of chronic pain associated with these disorders, as meas...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S12866
更新日期:2010-12-16 00:00:00
abstract:Purpose:The aim of this study was to evaluate postoperative discomfort in patients undergoing ambulatory strabismus under general anesthesia, and to identify risk factors associated with the discomfort. Patients and Methods:A cross-sectional study was conducted among 210 consecutive patients undergoing ambulatory stra...
journal_title:Journal of pain research
pub_type:
doi:10.2147/JPR.S249493
更新日期:2020-05-05 00:00:00
abstract:BACKGROUND:One form of self-medication is sharing of medications, defined as borrowing or lending medications in situations where the receiver of these drugs is not the individual to whom the medications were allocated. OBJECTIVE:To explore experiences and opinions of patients about sharing prescription analgesics, re...
journal_title:Journal of pain research
pub_type: 杂志文章
doi:10.2147/JPR.S123554
更新日期:2017-02-10 00:00:00