Abstract:
Objective:Sanguinarine (SNG) is a benzophenanthridine alkaloid obtained from the roots of Sanguinaria canadensis and has an anticancer effect. The aim of this study was to explore the mechanism of SNG in inhibiting macrophages via regulating the exosomes derived from lung carcinoma cells to reduce metastasis and proliferation of lung carcinoma. Methods:Human lung cancer cells (A549 cells) were treated with 4μM of SNG. Exosomes of A549 cells were extracted from A549 cells supernatant, and THP-1 cells were cultured with exosomes. Then, the supernatant of THP-1 cells was collected and cultured with A549 cells. Cell proliferation was measured via plate clone formation and CCK-8 assays. Migration was assessed by using Transwell assay and scratch test. Cellular invasion was detected by Transwell assay. Apoptosis was determined using flow cytometry. Moreover, the protein expressions of GAPDH, P65 and P-P65 in THP-1 cells were measured by Western blot. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and chemotactic cytokines ligand 2 (CCL-2) extracted from THP-1 cells were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results:Compared to the control group, exosomes could activate THP-1 cells, and the invasion, migration, and proliferation of A549 cells were consequently enhanced. Exosomes could increase the protein expression of p-p65 and the RNA expression levels of TNF-α, IL-6, and CCL-2 in THP-1 cells. Compared with the exosome group, SNG-treated exosomes inhibited THP-1 cells so that the invasion, proliferation, and migration of A549 cells were attenuated and apoptosis was promoted. In THP-1 cells, SNG-treated exosomes inhibited P-P65 expression and the RNA expression levels of TNF-α, IL-6, and CCL-2. Conclusion:Exosomes treated by SNG inhibited THP-1 cells so that the invasion, proliferation, and migration of A549 cells were inhibited, and the apoptosis was promoted. The mechanism is possibly associated with the inhibition of NF-κB pathway in THP-1 cells.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Yu Y,Luo Y,Fang Z,Teng W,Yu Y,Tian J,Guo P,Xu R,Wu J,Li Ydoi
10.2147/OTT.S261054subject
Has Abstractpub_date
2020-09-10 00:00:00pages
8989-9003issn
1178-6930pii
261054journal_volume
13pub_type
杂志文章abstract:Background:It has been reported that ribophorin II (RPN2) expression is increased in many cancers, but the role of RPN2 in nasopharyngeal carcinoma (NPC) remains unclear. Patients and methods:This study found that the expression of RPN2 is increased dramatically in NPC tissues of patients compared with that in the adj...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S158355
更新日期:2018-06-15 00:00:00
abstract::The prevalence of cancer remains high. With the improvement of diagnosis and treatment level and the increase of cancer survivors after treatment, multiple primary tumors are more common than before. The diagnosis and treatment of synchronous multiple primary tumors is more complicated than that of single or metachron...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S200625
更新日期:2019-04-02 00:00:00
abstract:BACKGROUND:Two types of epidermal growth factor receptor (EGFR) mutations in exon 19 and exon 21 (ex19del and L858R) are prevalent in lung cancer patients and sensitive to targeted EGFR inhibition. A resistance mutation in exon 20 (T790M) has been found to accompany drug treatment when patients relapse. These three mut...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S84938
更新日期:2015-06-22 00:00:00
abstract:Introduction:The purpose of present study was to investigate the effect of limonin on tumor glycolysis and the underlying mechanisms in hepatocellular carcinoma (HCC). Methods:Cell proliferation and colony formation assays were performed to evaluate the potency of limonin against HCC cells in vitro. The glucose consum...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S165220
更新日期:2018-07-03 00:00:00
abstract::Patients with papillary thyroid cancer (PTC) generally have good prognosis, but inoperable and radioactive iodine-refractory PTC still poses significant clinical challenges due to lack of effective treatment and higher mortality rates. Given the important role of integrins in multiple steps of tumor development, integ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S99166
更新日期:2016-03-11 00:00:00
abstract:Introduction:Odd-skipped related transcription factor 1 (OSR1) is a newly identified tumor suppressor in many tumor types. However, the role and mechanism of OSR1 in colon adenocarcinoma (COAD) remain unknown. Methods:OSR1 expression was detected in COAD tissues and cells. COAD cells with OSR1 overexpression or knockd...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S242386
更新日期:2020-04-24 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the therapeutic efficacy and safety of trastuzumab emtansine (T-DM1) for the treatment of patients with human epidermal growth factor receptor 2-positive breast cancer. METHODS:We performed a systemic review and meta-analysis of the relevant published clinical studies. A c...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S100499
更新日期:2016-02-29 00:00:00
abstract:Background:The aberrant expression of circular RNAs (circRNAs) has been identified as a novel trait of cancers. However, the role of circRNAs in colorectal cancer (CRC) remains to be elucidated. Methods:Informatic analysis was performed to identify circRNAs in CRC tissues and adjacent tissues. Gain- and loss-of-functi...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S255485
更新日期:2020-06-30 00:00:00
abstract::Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor, and surgical resection is the first choice for the treatment of the disease, but since the advent of tyrosine kinase inhibitors (TKIs) such as imatinib (IM), the prognosis of the disease has undergone revolutionary changes. According to the cu...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S279998
更新日期:2021-01-05 00:00:00
abstract:INTRODUCTION:The introduction of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) has improved the outlook for patients with advanced non-small-cell lung cancer (NSCLC) with EGFR+ mutations. However, most patients develop resistance, with the result that median progression-free survival (PFS) iŝ1...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S134382
更新日期:2017-05-05 00:00:00
abstract:BACKGROUND:C-X-C chemokine receptor type 4 (CXCR4) is involved in tumor progression including angiogenesis, metastasis, and survival. However, whether serum CXCR4 levels in metastatic or recurrent colorectal cancer have a prognostic role, have not been evaluated. METHODS:We analyzed serum samples from 55 patients with...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S104511
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:Current cancer treatments have unexpected side effects of which the death of normal cells is one. In some cancers, iron nanoparticles (NPs) can be subjected to diagnosis and passive targeting treatment. Cold atmospheric plasma (CAP) has a proven induction of selective cell death ability. In this study, we ha...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S95644
更新日期:2016-09-28 00:00:00
abstract:OBJECTIVE:To assess the need for pulmonary surgery in the treatment of refractory gestational trophoblastic neoplasia with lung metastasis after normalization of serum beta human chorionic gonadotropin (β-hCG) level with salvage chemotherapy. MATERIALS AND METHODS:A review of medical records of patients with refractor...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S56361
更新日期:2014-01-29 00:00:00
abstract::Angiogenesis (the growth of new blood vessels) is essential in most of the body's physiological processes, such as in the normal functioning of the endometrium during and after the menstrual cycle. Vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP) are the mostly expressed angiogenic factors,...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S132558
更新日期:2017-09-19 00:00:00
abstract:Background:Minichromosome maintenance protein 2 (MCM2), which is a member of MCM family, has been found to be a relevant marker for progression and prognosis in a variety of human cancers. Due to lack of effective therapeutic target in lung squamous cell carcinoma (LUSC) patients, the aim of our study was to screen and...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S169002
更新日期:2018-08-20 00:00:00
abstract::HES1 is the target of Notch signaling which is reported to affect cell differentiation and maintain the cells in G0 phase in various tissues including the hematopoietic tissue. HES1 expression appears to be an independent prognostic factor for survival in a heterogeneous group of acute myeloid leukemia (AML) patients....
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S83511
更新日期:2015-04-22 00:00:00
abstract:Background:The neutrophil-lymphocyte ratio (NLR), a biomarker for systematic inflammation, has been recently identified as a prognostic factor for various types of both solid and hematologic malignancies. Our study presented here was the first meta-analysis assessing the prognostic role of NLR in multiple myeloma (MM)....
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S153146
更新日期:2018-01-23 00:00:00
abstract:BACKGROUND:Gallbladder carcinoma is a highly malignant tumor and a public health problem in some parts of the world. It is characterized by a poor prognosis and its resistance to radio and chemotherapy. There is an urgent need to develop novel therapeutic alternatives for the treatment of gallbladder carcinoma. The mam...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S46897
更新日期:2013-10-03 00:00:00
abstract::Recently, "ipilimumab," an anti-cytotoxic T-lymphocyte antigen-4 (CTLA-4) monoclonal antibody, has been demonstrated to improve overall survival in metastatic melanoma. "CTLA-4" is an immune-checkpoint molecule that downregulates pathways of T-cell activation. Ipilimumab, by targeting CTLA-4, is able to remove the CTL...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S57335
更新日期:2014-02-19 00:00:00
abstract::Preclinical studies have demonstrated that Apatinib, major targeting vascular endothelial growth factor receptor-2 (VEGFR-2), could inhibit the proliferation of anaplastic thyroid carcinoma (ATC) cells in vitro and in vivo. The efficacy and safety in ATC patients, however, remains unknown. Here, we report the case of ...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S223727
更新日期:2019-12-02 00:00:00
abstract:Introduction:Cyclophosphamide, which is widely used to treat malignant disease, causes ovarian follicular atresia, which leads to premature ovarian insufficiency. The present study evaluated the protective effect of testosterone in preventing the decline in the ovarian reserve during cyclophosphamide treatment. Method...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S242703
更新日期:2020-04-08 00:00:00
abstract:Purpose:Primary squamous cell carcinoma is a rare malignancy in the thyroid gland (SCCTh). The overall prognosis of this carcinoma is poor. This study aimed to explore the application of Raman spectroscopy in investigating the expression of CK5/6 and P63 in SCCTh. Patients and Methods:Tissues of the SCCTh and adjacent...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S229436
更新日期:2020-01-20 00:00:00
abstract::Ocular sebaceous carcinoma (SC) is an exceedingly rare but aggressive malignancy that can impair patients' visual acuity or even cause tumor-specific mortality. This tumor usually mimics chronic benign eyelid lesions, thus leading to delayed diagnosis, thereby causing high recurrence and metastasis. Ophthalmologists s...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S162073
更新日期:2018-06-27 00:00:00
abstract::Defects in the innate immune system, particularly in Toll-like receptors (TLRs), have been reported in several cigarette smoke-promoted diseases. The aim of this study was to examine the impact of tobacco smoke on allelic frequencies of TLR4 single-nucleotide polymorphisms (SNPs) and to compare the genotypic distribut...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S111971
更新日期:2017-02-09 00:00:00
abstract:Background:The immunosuppressive facet and tumorigenic role of TNF-α have been revealed in osteosarcoma (OS). Long noncoding RNA THRIL is identified to regulate TNF-α expression and participates in immune response. Thus, investigations on the clinical expression pattern of THRIL/TNF-α signal in OS would provide a poten...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S235798
更新日期:2020-01-07 00:00:00
abstract:Purpose:The aim was to investigate resveratrol effects on A549 cells proliferation. Methods:A total of 104 lung adenocarcinoma tissues and nontumor tissues were collected. BEAS-2B cells were cultured in RPMI 1640 medium (group A). A549 cells were treated with RPMI 1640 medium containing different resveratrol concentra...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S157613
更新日期:2018-05-22 00:00:00
abstract:Background:The global Phase III LUX-Lung 8 trial (ClinicalTrials.gov: NCT01523587) identified significant improvements in progression-free survival (PFS), overall survival (OS), and patient-reported outcomes (PROs) with second-line afatinib vs erlotinib in patients with advanced squamous cell carcinoma (SCC) of the lun...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S161506
更新日期:2018-11-30 00:00:00
abstract:Background:The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progressio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S226078
更新日期:2019-10-31 00:00:00
abstract::[This retracts the article DOI: 10.2147/OTT.S232594.]. ...
journal_title:OncoTargets and therapy
pub_type: 撤回出版物
doi:10.2147/OTT.S283590
更新日期:2020-10-07 00:00:00
abstract:Background:Erdheim-Chester Disease (ECD) is a clonal non-Langerhans histiocytosis, classified as a macrophage-dendritic cell neoplasm in the 2016 WHO classification. The exact cell of origin of ECD is unknown, although some limited evidence suggests that it arises from myeloid progenitors. Case Presentation:A 43-year-...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S276497
更新日期:2020-11-16 00:00:00