Abstract:
Background:Large amounts of researches indicate that non-coding RNAs play a crucial role in many malignancies. However, the potential mechanisms of non-coding RNAs involved in osteosarcoma tumorigenesis remain elusive. Materials and Methods:The expression of long non-protein coding RNA 691 (lncRNA 691) in cell lines and paired osteosarcoma tissues was compared by qRT-PCR assay. Then, we explored the tumor suppressor function of lncRNA 691 with MTS and colony formation assay. Flow cytometry results showed lncRNA 691 can enhance cell apoptosis. Then, we predicted and verified the negative regulation relationship with miRNA and the miRNA's target gene. Lastly, we revealed the tumorigenesis function of lncRNA-691/miRNA/target gene axis in osteosarcoma. Results:In our study, we disclosed that lncRNA 691 had low expression levels in osteosarcoma cell lines and tissues. Overexpression of lncRNA 691 could suppress the cell proliferation and induce cell apoptosis in MG-63 cell line. Then, bioinformatics analyses were performed and miR-9-5p was found to negatively regulate the lncRNA 691 expression and promote the osteosarcoma tumorigenesis in vitro. PTEN was predicted as the target gene of miR-9-5p. Luciferase reporter assay and RIP assay demonstrated the regulatory network of lncRNA 691/miR-9-5p/PTEN. We revealed that PTEN was downregulated by the overexpression of miR-9-5p and upregulated by the overexpression of lncRNA 691. At last, the apoptosis-associated protein of the lncRNA 691/miR-9-5p/PTEN/PI3K/AKT was further demonstrated. Conclusion:LncRNA 691/miR-9-5p could regulate the tumorigenesis by regulating the PTEN/PI3K/AKT signal pathway in osteosarcoma.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Yao P,Ni Y,Liu Cdoi
10.2147/OTT.S249827subject
Has Abstractpub_date
2020-05-22 00:00:00pages
4597-4606issn
1178-6930pii
249827journal_volume
13pub_type
杂志文章abstract:Objective:Sanguinarine (SNG) is a benzophenanthridine alkaloid obtained from the roots of Sanguinaria canadensis and has an anticancer effect. The aim of this study was to explore the mechanism of SNG in inhibiting macrophages via regulating the exosomes derived from lung carcinoma cells to reduce metastasis and prolif...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S261054
更新日期:2020-09-10 00:00:00
abstract:Background:Patients with BRAF (v-Raf murine sarcoma viral oncogene homolog B) V600E-mutated metastatic colorectal cancer (mCRC) have a poor prognosis. The Southwest Oncology Group (SWOG) 1406 study evaluated the efficacy of vemurafenib in combination with irinotecan and cetuximab for simultaneous inhibition of epiderma...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S180845
更新日期:2019-01-08 00:00:00
abstract:BACKGROUND:Persistent infection with high-risk human papillomavirus (HPV) is a predominant cause of cervical cancer, and HPV58 is the third most common virus detected in the patients with cervical cancer in Asia. E6 and E7 are the viral oncogenes which are constitutively expressed in HPV-associated tumor cells and can ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S84888
更新日期:2015-10-23 00:00:00
abstract::Two decades ago, lymphatic mapping of sentinel lymph nodes (SLN) was introduced into surgical cancer management and was termed sentinel node navigated surgery. Although this technique is now routinely performed in the management of breast cancer and malignant melanoma, it is still under investigation for use in other ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S50394
更新日期:2014-06-24 00:00:00
abstract:Background:The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progressio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S226078
更新日期:2019-10-31 00:00:00
abstract:Introduction:Multiple myeloma (MM) remains an incurable disease, and patient survival requires a better understanding of this malignancy's molecular aspects. Heparanase (HPSE) is highly expressed in aggressive MM cells and related to tumor growth, metastasis, and bortezomib (BTZ) resistance. Thus, targeting HPSE seems ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S286751
更新日期:2021-01-15 00:00:00
abstract:Objective:We aimed to determine the safety and efficacy of irreversible electroporation (IRE) combined with chemotherapy for unresectable pancreatic carcinoma (stage III/IV). Methods:We prospectively enrolled 54 patients (30 men; median age 61.0 years; range 41-73 years) undergoing IRE with or without chemotherapy for...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S186721
更新日期:2019-02-18 00:00:00
abstract::Breast cancer is one of the most common malignant cancers among women and a major clinical obstacle. Although studies have reported the abnormal expression of SIRT7 in breast cancer, whether the function of SIRT7 regulates the expression of long noncoding RNAs (lncRNAs) in breast cancer remains unknown. We aimed to de...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S149048
更新日期:2017-10-24 00:00:00
abstract::High-mobility group AT-hook 2 (HMGA2) is involved in a wide spectrum of biological processes and is upregulated in several tumors, but its role in renal carcinoma remains unclear. The aim of this study was to examine the expression of HMGA2 and its relationship to the overall survival (OS) of patients with non-metasta...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S116953
更新日期:2016-11-22 00:00:00
abstract::Lung cancer is the leading cause of cancer-related mortality in both sexes, accounting for over one quarter of cancer deaths. Non-small-cell lung cancer (NSCLC) comprises 85%-90% of lung cancer diagnoses and despite advances in multimodality therapies, 5-year survival rates remain dismal with a median survival for pat...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S67262
更新日期:2015-04-20 00:00:00
abstract::The central nervous system (CNS) is regarded as an immune privileged environment; however, changes in the neuroimmunology paradigm have led to an increased interest in systematic immunotherapy in lung cancer therapy. The presence of the lymphatic system in the CNS as well as the physiological and biochemical changes i...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S235714
更新日期:2020-01-23 00:00:00
abstract::Background/Aims: MiR-145 and Smad2 have been widely reported in the development and progression of human malignancies. In the present study, we investigated the correlation between miR-145 and Smad2 in human glioblastoma multiforme (GBM). Methods: The epithelial-mesenchymal transition (EMT) biomarkers and Smad2 were a...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S202129
更新日期:2019-04-23 00:00:00
abstract:Purpose:This study is aimed to investigate the combined treating efficacy of sodium butyrate and docetaxel on proliferation and apoptosis of the lung adenocarcinoma A549 cell line based on Gli1 regulation in vitro and in vivo. Materials and Methods:RNA interference method was used to overexpress Gli1 in A549 cells. Ce...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S252323
更新日期:2020-09-04 00:00:00
abstract::Aberrant promoter methylation of RUNX3 has been reported in several tumors including human breast cancer (BC). However, the association between RUNX3 hypermethylation and incidence of BC remains elusive. In this study, a detailed literature search was performed in Medline and Google Scholar for related research public...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S77828
更新日期:2016-08-26 00:00:00
abstract:Background:Several EGFR-tyrosine kinase inhibitors (TKIs), such as gefitinib (Gef), have been used as effective clinical therapies for patients with non-small cell lung cancer (NSCLC). However, due to acquired resistance, the efficacy of Gef treatment is severely blocked. Our preliminary study found that epigallocatech...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S209441
更新日期:2019-07-26 00:00:00
abstract::The view that B lymphocytes are pathogenic in diverse pathological settings is supported by the efficacy of B-cell-ablative therapy in lymphoproliferative disorders, autoimmune diseases and graft rejection. Anti-B-cell antibodies (Abs) directed against CD20 have therefore been generated, and of these, rituximab was th...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/ott.s9774
更新日期:2010-06-24 00:00:00
abstract::Fibroblast growth factor receptor (FGFR) family includes four highly conserved receptor tyrosine kinases. Particularly, FGFR2 has been identified as a potential target for tyrosine kinase inhibitor (TKI) treatment. Except for immunohistochemistry and fluorescence in situ hybridization, next-generation sequencing (NGS)...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S218796
更新日期:2019-11-06 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate an intravoxel incoherent motion (IVIM) model-based analysis of diffusion-weighted imaging (DWI) for assessing the response of hepatocellular carcinoma (HCC) to locoregional therapy. PATIENTS AND METHODS:Respiratory-gated DWI (b=0, 50, and 800 s/mm2) was retrospectively ana...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S113909
更新日期:2016-10-19 00:00:00
abstract::[This corrects the article on p. 5203 in vol. 9, PMID: 27578986.]. ...
journal_title:OncoTargets and therapy
pub_type: 已发布勘误
doi:10.2147/OTT.S139588
更新日期:2017-04-27 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and safety of bortezomib-based vs non-bortezomib-based post-transplantation therapy in patients with multiple myeloma. METHODS:Data of relevant randomized controlled trials assessing the effect of bortezomib as post-transplantation consolidation or maintenance therapy was obtained th...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S84828
更新日期:2015-06-15 00:00:00
abstract:BACKGROUND:According to the relevant reports, TIMP-2 polymorphism might be associated with the susceptibility to gastric cancer. Owing to the inconclusive results from the published studies based on the association between TIMP-2 single nucleotide polymorphisms (SNPs) and gastric cancer susceptibility, a meta-analysis ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S114113
更新日期:2016-11-03 00:00:00
abstract:AIM:The aim of this study was to assess if the expression of Notch 1 receptor is associated with tumor aggressiveness in papillary thyroid carcinomas (PTCs). PATIENTS AND METHODS:By searching the electronic medical record system of Xin Hua Hospital, all cases of PTC patients who underwent thyroidectomy in the hospital...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S98239
更新日期:2016-03-15 00:00:00
abstract:Background:Anti-programmed cell death-1/programmed cell death ligand-1 monoclonal antibodies have been widely used in non-small-cell lung cancer (NSCLC), but not every patient can get benefits from them. Whether other molecular markers can predict the results of programmed cell death-1/programmed cell death ligand-1 in...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S164178
更新日期:2018-08-13 00:00:00
abstract:BACKGROUND:To evaluate the benefit of second-line chemotherapy with platinum-based treatment in patients with recurrent small cell lung cancer (SCLC). PATIENTS AND METHODS:A total of 535 patients continued with follow-up or best supportive care if needed, and 229 patients who progressed after the completion of first-l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S52330
更新日期:2013-10-22 00:00:00
abstract:BACKGROUND:Sunitinib is widely used as first-line treatment for metastatic clear cell renal cancer (MCRC). No reports are known of treatment after sunitinib failure. As irinotecan, cisplatin, and mitomycin-C (IPM) chemotherapy has been reported to influence MCRC after progression on cytokine therapy, we report on the o...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/ott.s3590
更新日期:2008-09-01 00:00:00
abstract:Background:Esophageal cancer (EC) is one of the aggressive gastrointestinal malignancies. It has been reported that microRNAs (miRNAs) play key roles during the tumorigenesis of EC. To identify novel potential targets for EC, differential expressed miRNAs (DEG) between EC and adjacent normal tissues were analyzed with ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S251508
更新日期:2020-09-24 00:00:00
abstract:Purpose:Gastric carcinoma is the second most frequently diagnosed cancer and leading cause of cancer death in China. As a new generation of cancer therapeutic drug, CL-6, a curcumin derivative, shows better bioavailability than curcumin, which has shown anticancer effects in gastric cancer (GC). However, whether CL-6 s...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S196914
更新日期:2019-03-27 00:00:00
abstract::Metastases from melanoma have a very poor prognosis for the patient. Single metastatic lesions in the sternum due to melanoma are extremely rare. A rare case of a presternal mass in a 56-year-old patient who had undergone excision for malignant melanoma is presented. Review of the patient's history and surgical resect...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S95723
更新日期:2016-01-18 00:00:00
abstract::This study tested the potential of circulating RNA-based signals as predictive biomarkers for docetaxel response in patients with metastatic castration-resistant prostate cancer (CRPC). RNA was analyzed in blood from six CRPC patients by whole-transcriptome sequencing (total RNA-sequencing) before and after docetaxel ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S86637
更新日期:2015-07-30 00:00:00
abstract:Background:miR520a-3p has previously had its antitumorigenic role in various types of cancers revealed, and been predicted as a posttranscriptional regulator of the NFκB-subunit RELA gene. Thus, miR520a-3p could function in carcinogenesis through suppressing RELA. Methods:Expression of miR520a-3p and RELA mRNA was qua...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S208889
更新日期:2019-08-14 00:00:00