当前位置： SCI文献检索 > Infection and Drug Resistance期刊下所有文献 > Rapid Carbapenemase Detection With Xpert Carba-R V2 Directly On Positive Blood Vials.

Rapid Carbapenemase Detection With Xpert Carba-R V2 Directly On Positive Blood Vials.

Abstract：

:The rapid detection of carbapenemase allows implementation of infection control measures and adaptation of antibiotic therapy. We evaluated the performances of the Xpert Carba-R V2® assay for the direct detection and identification of carbapenemase on positive blood cultures. We focused our evaluation on its detection capacity and on the risks of interference due to the patient's blood. Isolates of several variants of OXA-48-like (n=10), KPC (n=10), NDM (n=11), VIM (n=7), IMP-1 (n=1) carbapenemases and 14 non carbapenemase-producing Enterobacteriaceae were tested. For each isolate (n=53), an aerobic vial was seeded, and incubated in Bactec Fx (Becton Dickinson®) automate. When positive, the Xpert® Carba-R-V2 assay was assessed for carbapenemase detection using 40 µl aliquot. Reproducibility tests were performed on a subset of 23 isolates using aerobic and anaerobic vials. Longer incubation time was also evaluated on 6 isolates. A complementary prospective study in real-time testing of patient-derived clinical samples on 20 additional positive blood vials with Gram negative bacilli on direct examination was performed. Perfect sensitivity and specificity (100%) were observed regardless of the carbapenemase type, the blood vials used and the time of incubation. Xpert® Carba-R-V2 assay is suitable for the rapid detection of the main carbapenemase genes directly on positive blood vials. Its performances and rapid time analysis allow its use in routine to guide therapeutic choices and to implement infection control measures.

journal_name

Infect Drug Resist

journal_title

Infection and drug resistance

authors

Cointe A,Walewski V,Hobson CA,Doit C,Bidet P,Dortet L,Bonacorsi S,Birgy A

doi

10.2147/IDR.S204436

subject

Has Abstract

pub_date

2019-10-23 00:00:00

pages

3311-3316

issn

1178-6973

pii

204436

journal_volume

12

pub_type

杂志文章

相关文献

Infection and Drug Resistance文献大全