Abstract:
BACKGROUND:Repetitive genotyping is useful to assess the genetic evolution of non-small-cell lung cancer (NSCLC) during treatment, but the need for sampling by biopsy is a major obstacle. Digital polymerase chain reaction (PCR) is a promising procedure for the detection of mutant alleles in plasma of cancer patients. METHODS:This prospective study enrolled patients with NSCLC and known epidermal growth factor receptor (EGFR) mutations and who had experienced disease progression during ongoing EGFR-tyrosine kinase inhibitor (TKI) therapy. Eligible patients received daily gefitinib and either pemetrexed or S-1 every 3 weeks until disease progression or the development of unacceptable toxicity. Peripheral blood was collected before and after the combination therapy for digital PCR and hepatocyte growth factor measurement. RESULTS:From May 2012 to January 2014, nine patients with a median age of 67 (range 52-80) years were enrolled. Patterns of disease progression during adjacent EGFR-TKI therapy were acquired resistance, observed in seven patients, and primary resistance, observed in two patients. Known EGFR mutations were detected in plasma samples of six (67%) patients at study enrollment. Of these, T790M mutation was concurrently detected in three (50%) patients. Four patients underwent gefitinib plus pemetrexed therapy, and five patients underwent gefitinib and S-1 therapy. The median number of cycles delivered was five, and the median progression-free survival was 5.7 months. Efficacy outcomes did not differ between treatments. After the combination therapy, plasma T790M status changed to positive in two patients. Hepatocyte growth factor level did not significantly change through the combination therapy. CONCLUSION:The usefulness of monitoring the genetic evolution of EGFR-driven tumors using noninvasive procedures was demonstrated. Since continuation of EGFR-TKI therapy with cytotoxic agents has an acceptable tolerability and a possibility of inducing T790M mutation, the combination therapy may be useful for EGFR-mutant NSCLC resistant to EGFR-TKI therapy without T790M mutation.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Nakahara Y,Takagi Y,Hosomi Y,Kagei A,Yamamoto T,Sawada T,Yomota M,Okuma Y,Mikura S,Okamura Tdoi
10.2147/OTT.S105976subject
Has Abstractpub_date
2016-08-24 00:00:00pages
5287-95issn
1178-6930pii
ott-9-5287journal_volume
9pub_type
杂志文章abstract::Hepatocellular carcinoma (HCC) with inferior vena cava (IVC) tumor thrombus rarely occurs, its prognosis is extremely poor. A 49-year-old locally advanced HCC male with tumor thrombus extension into the IVC and the right atrium was successfully treated with stereotactic body radiotherapy (SBRT). A radiation dose of 40...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S208560
更新日期:2019-07-04 00:00:00
abstract:OBJECTIVE:Although our previous studies have confirmed that 1, 3, 8-trihydroxy-6-methylant hraquinone (emodin) inhibits migration and invasion in epithelial ovarian cancer (EOC) cells, the underlying molecular mechanism remains unknown. Here, the aim was to investigate the effects of emodin on EOC cells and to study fu...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S138217
更新日期:2017-07-19 00:00:00
abstract::The clinicopathological and prognostic significance of telomerase reverse transcriptase (TERT) promoter mutations have been widely investigated in thyroid cancer; however, the results are still discrepant. Systematic searches were performed in PubMed, Web of Science, Scopus, Ovid, and the Cochran Library databases for...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S116594
更新日期:2016-11-11 00:00:00
abstract:Background:Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNAs and mRNA need to be...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S208024
更新日期:2020-03-03 00:00:00
abstract:Background:Neuroblastoma, mainly affecting children, is a lethal malignancy arising from the developing sympathetic nervous system. The genetic etiology of neuroblastoma remains mostly obscure. High mobility group AT-hook 2 (HMGA2), an oncogenic gene, is up-regulated in many tumors. Single nucleotide polymorphisms (SNP...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S229975
更新日期:2020-01-15 00:00:00
abstract:OBJECTIVE:Resveratrol is a phytoestrogen with various antiproliferative and proapoptotic effects. This in vitro study aimed to analyze the effect of resveratrol on the viability and expression of modulators of apoptosis in GH3 pituitary adenoma cells of the rat. METHODS:GH3 cells were incubated with resveratrol concen...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S45154
更新日期:2013-09-16 00:00:00
abstract:Background:Anti-programmed cell death-1/programmed cell death ligand-1 monoclonal antibodies have been widely used in non-small-cell lung cancer (NSCLC), but not every patient can get benefits from them. Whether other molecular markers can predict the results of programmed cell death-1/programmed cell death ligand-1 in...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S164178
更新日期:2018-08-13 00:00:00
abstract::Historically, patients with locally advanced or metastatic melanoma have an extremely poor prognosis. In recent years, major breakthroughs in cutaneous melanoma treatment have led to remarkable improvements in patient outcomes. However, there are limited published data on the efficacy of these novel therapies in the t...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S177111
更新日期:2018-12-04 00:00:00
abstract:Purpose:The microRNA (miRNA) profile changes in the tumor-associated macrophages. However, the role of miR-106b-5p in the glioblastoma-associated macrophages is poorly understood. Materials and Methods:In our study, miR-106b-5p and M2 macrophage markers were detected by qRT-PCR and Western blotting in THP1 cells, with...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S238975
更新日期:2020-07-30 00:00:00
abstract:BACKGROUND:The purpose of this study was to investigate the prognosis of ipsilateral supraclavicular lymph node metastasis (ISLM) without evidence of distant metastases at diagnosis in Chinese women with breast cancer and to elucidate the clinical value of adjuvant radiotherapy. METHODS:We performed a retrospective an...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S56596
更新日期:2014-02-15 00:00:00
abstract:BACKGROUND:Due to the hypervascular nature of glioblastoma (GBM), antiangiogenic treatments, such as vatalanib, have been added as an adjuvant to control angiogenesis and tumor growth. However, evidence of progressive tumor growth and resistance to antiangiogenic treatment has been observed. To counter the unwanted eff...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S93790
更新日期:2016-03-09 00:00:00
abstract::The aim of this study was to evaluate the efficacy and toxicity of stereotactic body radiation therapy (SBRT) in the treatment of patients with liver metastases. Between August 2006 and July 2011, patients with 1-4 liver metastases were enrolled and treated with SBRT using the CyberKnife(®) system at Tianjin Medical U...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S58409
更新日期:2014-06-12 00:00:00
abstract::Dichloroacetate (DCA), a small molecule mitochondria-targeting agent, can penetrate the blood-brain barrier, showing potential therapeutic effects on brain tumors. Considering the effects of DCA on tumor cellular metabolism, penetrating across the blood-brain barrier, as well as having potential antitumor activity on ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S40992
更新日期:2013-01-01 00:00:00
abstract:Background:Penile squamous cell carcinoma (PSCC) bears poor prognosis due to its rarity and limited treatment options, especially after failure of standard treatments. Effective therapeutic options were desperately needed. Case Presentation:We report a recurrent metastatic PSCC patient with positive programmed death l...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S231258
更新日期:2020-04-21 00:00:00
abstract::Advances in therapies have led to the approval of six therapeutic agents since 2004, each demonstrating overall survival benefit in randomized studies, and these have significantly improved the outlook for men facing metastatic castration-resistant prostate cancer (CRPC). More recently, efforts have been directed at t...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S147168
更新日期:2018-04-12 00:00:00
abstract::The prevalence of cancer remains high. With the improvement of diagnosis and treatment level and the increase of cancer survivors after treatment, multiple primary tumors are more common than before. The diagnosis and treatment of synchronous multiple primary tumors is more complicated than that of single or metachron...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S200625
更新日期:2019-04-02 00:00:00
abstract:Purpose:Anaplastic thyroid cancer (ATC) is a kind of rare thyroid cancer with very poor prognosis. Doxorubicin has been approved in ATC treatment as a single agent, but monotherapy still shows no improvement of the total survival in advanced ATC. Lenvatinib was investigated with encouraging results in treating patients...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S278349
更新日期:2020-11-02 00:00:00
abstract::Recently, the human gene NOTCH1 has been found to be implicated in cancer cell metastasis and the maintenance of cancer stem cells. However, for breast cancer in particular, an association between NOTCH1 levels and metastasis has not been determined. In this study, we investigated the expression status and correlation...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S109606
更新日期:2016-11-07 00:00:00
abstract:Background:To investigate the effect of topical application of CpG oligodeoxynucleotide (CpG-ODN) combined with anti-4-1BB antibodies on mouse HCC multiple tumor-bearing models and the degree of improvement of anti-tumor immune response in mice. Materials and Methods:We inoculated each BALB/c male mouse subcutaneously...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S260353
更新日期:2020-07-20 00:00:00
abstract:Background:Lupeol, a triterpene isolated from various herbal plants, possesses an anti-inflammatory function and has been proposed as a candidate for anticancer agents. The purpose of this research was to investigate the effect of lupeol on the viability, apoptosis, cell-cycle distribution, and migration of colorectal ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,收录出版
doi:10.2147/OTT.S183925
更新日期:2018-11-09 00:00:00
abstract:Background:The aim of this study was to investigate the correlation of EGFR mutation on the high-resolution computed tomography (HRCT) features in lung adenocarcinoma. Patients and methods:A total of 121 patients were diagnosed with lung adenocarcinoma from January 2014 to December 2016. The correlation of indexes (ge...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S184217
更新日期:2019-01-10 00:00:00
abstract::Metastases from melanoma have a very poor prognosis for the patient. Single metastatic lesions in the sternum due to melanoma are extremely rare. A rare case of a presternal mass in a 56-year-old patient who had undergone excision for malignant melanoma is presented. Review of the patient's history and surgical resect...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S95723
更新日期:2016-01-18 00:00:00
abstract::This study tested the potential of circulating RNA-based signals as predictive biomarkers for docetaxel response in patients with metastatic castration-resistant prostate cancer (CRPC). RNA was analyzed in blood from six CRPC patients by whole-transcriptome sequencing (total RNA-sequencing) before and after docetaxel ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S86637
更新日期:2015-07-30 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the use of (18)F-fluorodeoxyglucose positron emission tomography/computed tomography ((18)F-FDG PET/CT) to identify the presence of cervical lymph nodes metastases and extracapsular spread with histologic correlations in head and neck squamous cell carcinoma. METH...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S85479
更新日期:2015-08-26 00:00:00
abstract::Targeted therapies against EGFR, vascular endothelial growth factor, and vascular endothelial growth factor receptor have expanded treatment options for patients with metastatic colorectal cancer (mCRC). Unfortunately, biomarkers to identify patients that are most likely to derive benefit from targeted therapies in th...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S68558
更新日期:2015-12-30 00:00:00
abstract::Primary urethral non-Hodgkin's lymphoma (NHL) is uncommon. This case study reports the case of a 52-year-old woman with a light red mass at the urethral orifice. Her clinical manifestations included frequent urination, urgency, dysuria, and occasionally blood in urine. The tumor was surgically removed, and the patholo...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S283704
更新日期:2020-12-21 00:00:00
abstract:Background:The diagnosis rate of early stage esophageal squamous cell carcinoma (ESCC) is low due to the lack of specific tumor markers. Seeking for these markers is beneficial to improve the early diagnosis rate and the prognosis of patients. This study profiles the differentially expressed proteins of early stage ESC...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S171242
更新日期:2018-09-12 00:00:00
abstract:Background/aim:Urothelial bladder cancer arises from the accumulation of multiple epigenetic and genetic changes. We aimed to investigate the specificity and sensitivity of gene-specific promoter methylation of CDH1 and p14ARF genes in the early diagnosis of bladder cancer and compare those with other diagnostic tests ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S158259
更新日期:2018-07-19 00:00:00
abstract:Purpose:LINC00680 was reported to be involved in various cancers through multiple mechanisms. Therefore, we intended to investigate its role in the progression of non-small cell lung cancer (NSCLC). Materials and Methods:Firstly, quantitative real-time polymerase chain reaction (qRT-PCR) was used to test LINC00680 in ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S259232
更新日期:2020-08-18 00:00:00
abstract::Lung cancer has the highest morbidity and mortality in the world, and non-small cell lung carcinomas (NSCLC) account for 80% of cases of lung cancer. The mechanism of NSCLC is still largely unknown, and finding novel targets is of great importance for the treatment of NSCLC. The current study was designed to evaluate ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S123518
更新日期:2017-03-02 00:00:00