Abstract:
Background:In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of patients is still a challenge. For these reasons, it is necessary to explore new cellular sources and immunological isolation methods oriented to develop T1DM cell-based therapies. Aims:We postulate human adipose-derived stem cell (hASC) as an adequate source to generate pancreatic islet cells in vitro, and to produce islet-like structures. Furthermore, we propose microencapsulation of these aggregates as an immunological isolation strategy. Methods:hASC obtained from lipoaspirated fat tissue from human donors were differentiated in vitro to insulin (Ins) and glucagon (Gcg) producing cells. Then, insulin producing cells (IPC) and glucagon producing cells (GPC) were cocultured in low adhesion conditions to form cellular aggregates, and later encapsulated in a sodium alginate polymer. Expression of pancreatic lineage markers and secretion of insulin or glucagon in vitro were analyzed. Results:The results show that multipotent hASC efficiently differentiate to IPC and GPC, and express pancreatic markers, including insulin or glucagon hormones which they secrete upon stimulation (fivefold for insulin in IPC, and fourfold for glucagon, compared to undifferentiated cells). In turn, calculation of the Feret diameter and area of cellular aggregates revealed mean diameters of ~ 80 µm, and 65% of the aggregates reached 4000 µm2 at 72 h of formation. IPC/GPC aggregates were then microencapsulated in sodium-alginate polymer microgels, which were found to be more stable when stabilized with Ba2+, yielding average diameters of ~ 300 µm. Interestingly, Ba2+-microencapsulated aggregates respond to high external glucose with insulin secretion. Conclusions:The IPC/GPC differentiation process from hASC, followed by the generation of cellular aggregates that are later microencapsulated, could represent a possible treatment for T1DM.
journal_name
Diabetol Metab Syndrjournal_title
Diabetology & metabolic syndromeauthors
Jara C,Oyarzun-Ampuero F,Carrión F,González-Echeverría E,Cappelli C,Caviedes Pdoi
10.1186/s13098-020-00573-9subject
Has Abstractpub_date
2020-08-05 00:00:00pages
66issn
1758-5996pii
573journal_volume
12pub_type
杂志文章abstract:BACKGROUND:We investigated the influence of aerobic capacity on the improvement in glycemic control achieved by long-term aerobic exercise in type 2 diabetes. METHODS:Fifty-three male patients with type 2 diabetes, recruited from outpatient clinics, wore multiple-memory accelerometers and were instructed to exercise a...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-017-0262-9
更新日期:2017-08-18 00:00:00
abstract:BACKGROUND:Many patients with chronic heart failure (CHF) are believed to have unrecognized diabetes, which is associated with a worse prognosis. This study aimed to describe glucose tolerance in a general stable CHF population and to identify determinants of glucose tolerance focusing on body composition and skeletal ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-44
更新日期:2014-03-27 00:00:00
abstract:Background:Adipokines are involved in the pathogenesis of metabolic disorders including obesity and type 2 diabetes mellitus (T2DM). This study investigates the levels of leptin, resistin, visfatin, secreted frizzled-related protein 5 (SFRP5), monocyte chemoattractant protein-1 (MCP-1) and retinol-binding protein 4 (RB...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-018-0343-4
更新日期:2018-05-15 00:00:00
abstract:Background:Diabetic nephropathy (DN) is a severe complication of diabetes with type 1 and 2. Long non-coding RNAs (lncRNAs) are being found to be involved in the DN pathogenesis. In this study, we aimed to further explore the effect and underlying mechanism of plasmacytoma variant translocation 1 (PVT1) in DN pathogene...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00539-x
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Vildagliptin, a DPP-4 inhibitor widely used for the treatment of type 2 diabetes mellitus (T2DM), shows beneficial effects on endothelial function. This study aims to evaluate the effect of vildagliptin on endothelial function and arterial stiffness in patients with T2DM and hypertension. METHODS:Fifty over...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0062-z
更新日期:2015-08-26 00:00:00
abstract:BACKGROUND:Polymorphisms in genes encoding adiponectin (ADIPOQ) and interleukin-6 (IL6) have been associated with adiposity and obese-related phenotypes. This study investigated the relationship of ADIPOQ and IL6 gene polymorphisms with pro-inflammatory and cardiometabolic markers in obese patients. METHODS:Anthropome...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0027-2
更新日期:2015-04-11 00:00:00
abstract::Insulin resistance and hypertension are considered as prototypical "diseases of civilization" that are manifested in the modern environment as plentiful food and sedentary life. The human propensity for insulin resistance and hypertension is a product, at least in part, of our evolutionary history. Adaptation to ancie...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-12
更新日期:2014-01-31 00:00:00
abstract:BACKGROUND:This study aimed to determine self-monitoring practices, awareness to dietary modifications and barriers to medication adherence among physically disabled type 2 diabetes mellitus patients. METHODS:Interview sessions were conducted at diabetes clinic-Penang general hospital. The invited participants represe...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-017-0257-6
更新日期:2017-07-24 00:00:00
abstract:BACKGROUND:Polymorphisms of vitamin D receptor (VDR) gene have been studied as genetic markers of type 1 diabetes mellitus (T1DM) and some studies have reported associations with autoimmune thyroid disease. The aim of this study was to evaluate the relationship between VDR FokI polymorphism (rs10735810), thyroid autoim...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-016-0145-5
更新日期:2016-03-22 00:00:00
abstract:BACKGROUND:Hypertriglyceridemia (HTG) is considered an independent risk factor for major adverse cardiovascular events (MACE). METHODS:This study analyzed the effects of various agents on MACE risk reduction in HTG (serum triglyceride ≥ 150 mg/dl) populations by performing a network meta-analysis. We performed a frequ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-021-00626-7
更新日期:2021-01-29 00:00:00
abstract::The development of extended-action insulin analogues was motivated by the unfavorable pharmacokinetic (PK) profile of the conventional long-acting insulin formulations, generally associated with marked inter and intra patient variability and site- and dose-dependent effect variation. The new ultra-long insulin analogu...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0037-0
更新日期:2015-06-26 00:00:00
abstract:Background:Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in patients with type 2 diabetes (T2DM). Carotid intima-media thickness (CIMT) is considered a preclinical stage of atherosclerosis. Therefore, it is necessary to identify the related risk factors for CIMT to facilitate the early pr...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-0517-8
更新日期:2020-01-28 00:00:00
abstract:OBJECTIVE:To translate and validate the instrument Diabetes Self-Management Profile (DSMP)-Conventional and Flexible Regimens into Brazilian Portuguese language in order to evaluate the quality of diabetes self-management in children and adolescents with type 1 diabetes and their caregivers. METHODS:DSMP was submitted...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-017-0250-0
更新日期:2017-07-11 00:00:00
abstract:Objectives:To evaluate the effect of Lactobacillus casei 01 on dietary intake, body weight, and glycemic control in patients with T2DM. Method:Forty patients with T2DM (n = 20 for each group) were assigned into two groups in present trial. The patients in the probiotic group received a daily capsule containing a minim...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0400-7
更新日期:2019-01-15 00:00:00
abstract:BACKGROUND:Liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, has been shown to possess pleiotropic effects including body weight reduction. However, long-term effect of liraglutide on body weight and glycemic control has not been elucidated in Japanese type 2 diabetes (T2D) subjects. Present study investigates w...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-95
更新日期:2014-09-08 00:00:00
abstract:Background:Population attributable fractions (PAFs) are frequently used to quantify the proportion of Type 2 diabetes cases due to single risk factors, an approach which may result in an overestimation of their individual contributions. This study aimed to examine Type 2 diabetes incidence associated with multiple risk...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-018-0387-5
更新日期:2018-11-22 00:00:00
abstract::Latin America is a fast-growing region that currently faces unique challenges in the treatment of all forms of diabetes mellitus. The burden of this disease will be even greater in the coming years due, in part, to the large proportion of young adults living in urban areas and engaging in unhealthy lifestyles. Unfortu...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章,评审
doi:10.1186/s13098-019-0415-0
更新日期:2019-02-18 00:00:00
abstract:BACKGROUND:Angiotensin-converting enzyme (ACE) inhibitors are widely prescribed for patients with diabetes as a nephroprotector drug or to treat hypertension. Generally they are safe for clinical practice, but the relationship between these drugs and angioedema is known. The exact mechanism for ACE inhibitors-induced a...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-2-4
更新日期:2010-01-20 00:00:00
abstract:BACKGROUND:The oral DPP-4 inhibitors are new incretin-based therapies for treatment of type 2 diabetes. To assess the efficacy and safety of three DPP-4 inhibitors (Saxagliptin, Sitagliptin and Vildagliptin) as add-on therapy to dual combination of traditional oral hypoglycemic agents in Chinese type 2 diabetes patient...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-69
更新日期:2014-05-31 00:00:00
abstract:BACKGROUND:Pregnant women with mild gestational hyperglycemia present a high risk for hypertension and obesity, and appear to reproduce the model of metabolic syndrome in pregnancy, including hyperinsulinemia and insulin resistance. Diabetic patients have a higher frequency of the IRS-1 Gly972Arg variant and this polym...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0026-3
更新日期:2015-04-02 00:00:00
abstract:: Metabolic Syndrome (MS) increases the risk for Coronary Artery Disease, stroke and diabetes. MS is twice more common amongst South Asian immigrants in US compared to native Caucasians. There are no nationally representative studies on prevalence of MS from any of the South Asian countries. The present study aims to e...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-4-24
更新日期:2012-05-31 00:00:00
abstract:Background:Diabetic nephropathy (DN) or diabetic kidney disease refers to the deterioration of kidney function seen in chronic type 1 and type 2 diabetes mellitus patients. The progression of the disease is known to occur in a series of stages and is linked to glycemic and blood pressure control. However, despite aggre...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章,评审
doi:10.1186/s13098-019-0403-4
更新日期:2019-01-23 00:00:00
abstract:BACKGROUND:An increase in the prevalence of obesity entails great expenditure for governments. Physical exercise is a powerful tool in the combat against obesity and obesity-associated diseases. This study sought to determine the effect of three different exercise protocols on metabolic syndrome and lipid peroxidation ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-3-35
更新日期:2011-12-19 00:00:00
abstract:: The scope of this short review is to summarise the knowledge gleaned from the fate of drugs transported by albumin upon contact with the target cancer cell or cells in inflamed tissues. The authors expertise covers covalently bound drugs and their cellular uptake and release from albumin. This review therefore aims t...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-3-11
更新日期:2011-06-15 00:00:00
abstract:BACKGROUND:Comorbid depression in diabetes has been suggested as one of the possible causes of an inadequate glycemic control. The purpose of this study was to investigate the association between major depression and the glycemic control of type 2 diabetes mellitus (T2DM). METHODS:Seventy T2DM patients were evaluated....
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-3-26
更新日期:2011-10-07 00:00:00
abstract::This manuscript reports the Brazilian Diabetes Society Position Statement for insulin adjustments based on trend arrows observed in continuous glucose monitoring systems. The Brazilian Diabetes Society supports the utilization of trend arrows for insulin dose adjustments in patients with diabetes on basal-bolus insuli...
journal_title:Diabetology & metabolic syndrome
pub_type: 信件
doi:10.1186/s13098-020-00607-2
更新日期:2021-01-03 00:00:00
abstract:BACKGROUND:Diabetes treatment requires specialized multi-professional teams, supplies for blood glucose monitoring and training for self-injections of human insulin or insulin analogues. The State Health Secretariat of the Federal District (SHS-FD) has dispensed insulin analogues by means of clinical validated protocol...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0061-0
更新日期:2015-08-18 00:00:00
abstract:: In recent decades, Diabetes Mellitus has become a severe and growing global public healthcare problem due to the increase of its prevalence, morbidity and mortality. Post-transplant diabetes mellitus (PTDM) is a complication which takes place after a solid organ transplant, and its incidence is widely variable, rangi...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-1-14
更新日期:2009-10-05 00:00:00
abstract:BACKGROUND:Helicobacter pylori (H. pylori) infection is reported to be associated with various extragastrointestinal conditions such as insulin resistance, diabetes mellitus and metabolic syndrome. H. pylori infection and type 2 diabetes mellitus (T2DM) are associated with oxidative stress, this cross-relation between ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-016-0149-1
更新日期:2016-05-03 00:00:00
abstract:Background:Previous studies comparing insulin degludec/insulin aspart (IDegAsp) with premixed insulin twice daily among insulin users with type 2 diabetes have not thoroughly investigated differences in the glucose variability and psychological evaluations related to insulin regimen changes. We investigated changes in ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-018-0366-x
更新日期:2018-08-16 00:00:00