当前位置： SCI文献检索 > Viruses-Basel期刊下所有文献 > Replication of a Dog-Origin H6N1 Influenza Virus in Cell Culture and Mice.

Replication of a Dog-Origin H6N1 Influenza Virus in Cell Culture and Mice.

Abstract：

:The world's first natural avian-origin H6N1 influenza A virus infection case in dogs was confirmed in Taiwan in 2014. The H6N1 virus in chickens has been endemic in Taiwan since 1972. Whether the dog H6N1 virus has interspecies transmission potential is the key issue we aim to understand. Following one virus passage in embryonated eggs and two further passages in MDCK cells, we obtained two virus derivatives, E01EE (PB1 739E and PB2 627E) and E01GK (PB1 739G and PB2 627K), respectively. The pathogenicity of E01EE and E01GK was investigated using plaque assay, growth dynamic analysis and cell viability quantification in cells from different animal species. The impact of amino acid mutation on PB1 739 and PB2 627 on viral ribonucleoprotein (RNP) activity was also analyzed. Further mouse infection experiments were performed. The results showed that both E01EE and E01GK decreased cell relative viability of canine MDCK cells, human A549 cells and chicken DF1 cells. E01Gk caused greater cellular harm in MDCK and A549 cells and had significantly higher virus titers in all of the cells compared to E01EE. The PB2 627K but not PB1 739G was the critical mutation that influenced the viral RNP activity. Both E01EE and E01GK caused mice pneumonia and considerable virus shedding, especially E01GK. This report verifies PB2 E627K mutation in virulence and spotlights the potential for the dog H6N1 virus to extend interspecies transmission.

journal_name

Viruses

journal_title

Viruses

authors

Tsai SK,Shih CH,Chang HW,Teng KH,Hsu WE,Lin HJ,Lin HY,Huang CH,Chen HW,Wang LC

doi

10.3390/v12070704

subject

Has Abstract

pub_date

2020-06-30 00:00:00

issue

7

issn

1999-4915

pii

v12070704

journal_volume

12

pub_type

杂志文章

相关文献

Viruses-Basel文献大全
  • miRNAs as Potential Biomarkers for Viral Hepatitis B and C.

    abstract：:Around 257 million people are living with hepatitis B virus (HBV) chronic infection and 71 million with hepatitis C virus (HCV) chronic infection. Both HBV and HCV infections can lead to liver complications such as cirrhosis and hepatocellular carcinoma (HCC). To take care of these chronically infected patients, one s...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v12121440

    authors： Loureiro D,Tout I,Narguet S,Benazzouz SM,Mansouri A,Asselah T

    更新日期：2020-12-14 00:00:00

  • Influence of Irradiance and Temperature on the Virus MpoV-45T Infecting the Arctic Picophytoplankter Micromonas polaris.

    abstract：:Arctic marine ecosystems are currently undergoing rapid changes in temperature and light availability. Picophytoplankton, such as Micromonas polaris, are predicted to benefit from such changes. However, little is known about how these environmental changes affect the viruses that exert a strong mortality pressure on t...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10120676

    authors： Piedade GJ,Wesdorp EM,Montenegro-Borbolla E,Maat DS,Brussaard CPD

    更新日期：2018-11-29 00:00:00

  • The role of bacterial chaperones in the circulative transmission of plant viruses by insect vectors.

    abstract：:Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v5061516

    authors： Kliot A,Ghanim M

    更新日期：2013-06-19 00:00:00

  • Canine Influenza Virus is Mildly Restricted by Canine Tetherin Protein.

    abstract：:Tetherin (BST2/CD317/HM1.24) has emerged as a key host-cell ·defence molecule that acts by inhibiting the release and spread of diverse enveloped virions from infected cells. We analysed the biological features of canine tetherin and found it to be an unstable hydrophilic type I transmembrane protein with one transmem...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10100565

    authors： Zheng Y,Hao X,Zheng Q,Lin X,Zhang X,Zeng W,Ding S,Zhou P,Li S

    更新日期：2018-10-16 00:00:00

  • A Lineage of Begomoviruses Encode Rep and AC4 Proteins of Enigmatic Ancestry: Hints on the Evolution of Geminiviruses in the New World.

    abstract：:The begomoviruses (BGVs) are plant pathogens that evolved in the Old World during the Cretaceous and arrived to the New World (NW) in the Cenozoic era. A subgroup of NW BGVs, the "Squash leaf curl virus (SLCV) lineage" (S-Lin), includes viruses with unique characteristics. To get clues on the evolutionary origin of th...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11070644

    authors： Torres-Herrera SI,Romero-Osorio A,Moreno-Valenzuela O,Pastor-Palacios G,Cardenas-Conejo Y,Ramírez-Prado JH,Riego-Ruiz L,Minero-García Y,Ambriz-Granados S,Argüello-Astorga GR

    更新日期：2019-07-13 00:00:00

  • Zoonotic Hepatitis E Virus: Classification, Animal Reservoirs and Transmission Routes.

    abstract：:During the past ten years, several new hepatitis E viruses (HEVs) have been identified in various animal species. In parallel, the number of reports of autochthonous hepatitis E in Western countries has increased as well, raising the question of what role these possible animal reservoirs play in human infections. The a...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8100270

    authors： Doceul V,Bagdassarian E,Demange A,Pavio N

    更新日期：2016-10-03 00:00:00

  • Anti-CMV-IgG positivity of donors is beneficial for alloHSCT recipients with respect to the better short-term immunological recovery and high level of CD4+CD25high lymphocytes.

    abstract：:Hematopoietic stem cell transplantation from anti-cytomegalovirus immunoglobulin G (anti-CMV-IgG) positive donors facilitated immunological recovery post-transplant, which may indicate that chronic CMV infection has an effect on the immune system. This can be seen in the recipients after reconstitution with donor lymp...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v7031391

    authors： Jaskula E,Dlubek D,Tarnowska A,Lange J,Mordak-Domagala M,Suchnicki K,Sedzimirska M,Borowik A,Mizia S,Lange A

    更新日期：2015-03-23 00:00:00

  • An Oleanolic Acid Derivative Inhibits Hemagglutinin-Mediated Entry of Influenza A Virus.

    abstract：:Influenza A viruses (IAV) have been a major public health threat worldwide, and options for antiviral therapy become increasingly limited with the emergence of drug-resisting virus strains. New and effective anti-IAV drugs, especially for highly pathogenic influenza, with different modes of action, are urgently needed...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12020225

    authors： Ye M,Liao Y,Wu L,Qi W,Choudhry N,Liu Y,Chen W,Song G,Chen J

    更新日期：2020-02-18 00:00:00

  • Photodynamic inactivation of mammalian viruses and bacteriophages.

    abstract：:Photodynamic inactivation (PDI) has been used to inactivate microorganisms through the use of photosensitizers. The inactivation of mammalian viruses and bacteriophages by photosensitization has been applied with success since the first decades of the last century. Due to the fact that mammalian viruses are known to p...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v4071034

    authors： Costa L,Faustino MA,Neves MG,Cunha A,Almeida A

    更新日期：2012-07-01 00:00:00

  • Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds.

    abstract：:A Pseudorabies virus (PRV) variant has emerged in China since 2011 that is not protected by commercial vaccines, and has not been well studied. The PRV genome is large and difficult to manipulate, but it is feasible to use clustered, regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology. However, id...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v8040090

    authors： Tang YD,Liu JT,Fang QQ,Wang TY,Sun MX,An TQ,Tian ZJ,Cai XH

    更新日期：2016-03-29 00:00:00

  • Modes of Human T Cell Leukemia Virus Type 1 Transmission, Replication and Persistence.

    abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is a retrovirus that causes cancer (Adult T cell Leukemia, ATL) and a spectrum of inflammatory diseases (mainly HTLV-associated myelopathy-tropical spastic paraparesis, HAM/TSP). Since virions are particularly unstable, HTLV-1 transmission primarily occurs by transfer of a c...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v7072793

    authors： Carpentier A,Barez PY,Hamaidia M,Gazon H,de Brogniez A,Perike S,Gillet N,Willems L

    更新日期：2015-07-07 00:00:00

  • Orchestrating the Selection and Packaging of Genomic RNA by Retroviruses: An Ensemble of Viral and Host Factors.

    abstract：:Infectious retrovirus particles contain two copies of unspliced viral RNA that serve as the viral genome. Unspliced retroviral RNA is transcribed in the nucleus by the host RNA polymerase II and has three potential fates: (1) it can be spliced into subgenomic messenger RNAs (mRNAs) for the translation of viral protein...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8090257

    authors： Kaddis Maldonado RJ,Parent LJ

    更新日期：2016-09-20 00:00:00

  • Bovine Lactoferrin Prevents Influenza A Virus Infection by Interfering with the Fusogenic Function of Viral Hemagglutinin.

    abstract：:Bovine lactoferrin (bLf) is an iron-binding glycoprotein folded in two symmetric globular lobes (N- and C-lobes) with potent antimicrobial and immunomodulatory activities. Recently, we have shown that bLf, and in particular its C-lobe, interacts with influenza A virus hemagglutinin and prevents infection by different ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11010051

    authors： Superti F,Agamennone M,Pietrantoni A,Ammendolia MG

    更新日期：2019-01-11 00:00:00

  • Genetic Susceptibility to Human Norovirus Infection: An Update.

    abstract：:Noroviruses are the most common etiological agent of acute gastroenteritis worldwide. Despite their high infectivity, a subpopulation of individuals is resistant to infection and disease. This susceptibility is norovirus genotype-dependent and is largely mediated by the presence or absence of human histo-blood group a...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v11030226

    authors： Nordgren J,Svensson L

    更新日期：2019-03-06 00:00:00

  • Role of Herpes Simplex Envelope Glycoprotein B and Toll-Like Receptor 2 in Ocular Inflammation: An ex vivo Organotypic Rabbit Corneal Model.

    abstract：:It has been recently reported, using in vitro studies, that the herpes simplex virus 1 (HSV-1) encoded envelope glycoprotein B (gB1) interacts with cell surface toll-like receptor 2 (TLR2) and induces the secretion of interleukin-8 (IL8), a representative marker of inflammatory cytokine activation. The purpose of this...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11090819

    authors： Marino A,Pergolizzi S,Cimino F,Lauriano ER,Speciale A,D'Angelo V,Sicurella M,Argnani R,Manservigi R,Marconi P

    更新日期：2019-09-04 00:00:00

  • Recombinant Modified Vaccinia Virus Ankara (MVA) Vaccines Efficiently Protect Cockatiels Against Parrot Bornavirus Infection and Proventricular Dilatation Disease.

    abstract：:Parrot bornaviruses (PaBVs) are the causative agents of proventricular dilatation disease (PDD), a chronic and often fatal neurologic disorder in Psittaciformes. The disease is widely distributed in private parrot collections and threatens breeding populations of endangered species. Thus, immunoprophylaxis strategies ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11121130

    authors： Rall I,Amann R,Malberg S,Herden C,Rubbenstroth D

    更新日期：2019-12-06 00:00:00

  • Human Metapneumovirus Small Hydrophobic Protein Inhibits Interferon Induction in Plasmacytoid Dendritic Cells.

    abstract：:Human metapneumovirus (hMPV), a leading cause of respiratory tract infections in infants, encodes a small hydrophobic (SH) protein of unknown function. Here we show that infection of plasmacytoid dendritic cells (pDCs) with a recombinant virus lacking SH expression (rhMPV-ΔSH) enhanced the secretion of type I interfer...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10060278

    authors： Bao X,Kolli D,Esham D,Velayutham TS,Casola A

    更新日期：2018-05-23 00:00:00

  • Characterization of a Novel Megalocytivirus Isolated from European Chub (Squalius cephalus).

    abstract：:A novel virus from moribund European chub (Squalius cephalus) was isolated on epithelioma papulosum cyprini (EPC) cells. Transmission electron microscopic examination revealed abundant non-enveloped, hexagonal virus particles in the cytoplasm of infected EPC cells consistent with an iridovirus. Illumina MiSeq sequence...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11050440

    authors： Halaly MA,Subramaniam K,Koda SA,Popov VL,Stone D,Way K,Waltzek TB

    更新日期：2019-05-15 00:00:00

  • Experimental Infection of Pregnant Female Sheep with Zika Virus During Early Gestation.

    abstract：:Zika virus (ZIKV) is a vertically and sexually transmissible virus resulting in severe congenital malformation. The goal of this study was to develop an ovine model of ZIKV infection. Between 28-35 days gestation (DG), four pregnant animals were infected with two doses of 6 × 106 PFU of ZIKV; four control animals rece...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11090795

    authors： Schwarz ER,Pozor MA,Pu R,Barr KL,Beachboard SE,MacLachlan NJ,Prakoso D,Long MT

    更新日期：2019-08-29 00:00:00

  • THO Complex Subunit 7 Homolog Negatively Regulates Cellular Antiviral Response against RNA Viruses by Targeting TBK1.

    abstract：:RNA virus invasion induces a cytosolic RIG-I-like receptor (RLR) signaling pathway by promoting assembly of the Mitochondrial antiviral-signaling protein (MAVS) signalosome and triggers the rapid production of type I interferons (IFNs) and proinflammatory cytokines. During this process, the pivotal kinase TANK binding...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11020158

    authors： He TS,Xie T,Li J,Yang YX,Li C,Wang W,Cao L,Rao H,Ju C,Xu LG

    更新日期：2019-02-15 00:00:00

  • An Insight into Cotton Leaf Curl Multan Betasatellite, the Most Important Component of Cotton Leaf Curl Disease Complex.

    abstract：:Cotton leaf curl disease (CLCuD) is one of the most economically important diseases and is a constraint to cotton production in major producers, Pakistan and India. CLCuD is caused by monopartite plant viruses belonging to the family Geminiviridae (genus Begomovirus), in association with an essential, disease-specific...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v9100280

    authors： Zubair M,Zaidi SS,Shakir S,Amin I,Mansoor S

    更新日期：2017-09-29 00:00:00

  • The Impacts of Low Diversity Sequence Data on Phylodynamic Inference during an Emerging Epidemic.

    abstract：:Phylodynamic inference is a pivotal tool in understanding transmission dynamics of viral outbreaks. These analyses are strongly guided by the input of an epidemiological model as well as sequence data that must contain sufficient intersequence variability in order to be informative. These criteria, however, may not be...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v13010079

    authors： Lam A,Duchene S

    更新日期：2021-01-08 00:00:00

  • Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia.

    abstract：:Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillan...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12090963

    authors： Chambaro HM,Sasaki M,Simulundu E,Silwamba I,Sinkala Y,Gonzalez G,Squarre D,Fandamu P,Lubaba CH,Munyeme M,Maseko A,Chimvwele C,Mataa L,Mooya LE,Mukubesa AN,Harima H,Samui KL,Munang'andu HM,Simuunza M,Nalubamba KS,Q

    更新日期：2020-08-31 00:00:00

  • Evaluating the Within-Host Dynamics of Ranavirus Infection with Mechanistic Disease Models and Experimental Data.

    abstract：:Mechanistic models are critical for our understanding of both within-host dynamics (i.e., pathogen replication and immune system processes) and among-host dynamics (i.e., transmission). Within-host models, however, are not often fit to experimental data, which can serve as a robust method of hypothesis testing and hyp...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11050396

    authors： Mihaljevic JR,Greer AL,Brunner JL

    更新日期：2019-04-27 00:00:00

  • Brockman, M.A., et al., Human Leukocyte Antigen (HLA) Class I Down-Regulation by Human Immunodeficiency Virus Type 1 Negative Factor (HIV-1 Nef): What Might We Learn From Natural Sequence Variants? Viruses 2012, 4, 1711-1730.

    abstract：:In the original manuscript, the text in figure 1 is illegible. Furthermore, there is an unnecessary carriage return (page 1716, ~line 18) "crystallographic ... methods". ...

    journal_title：Viruses

    pub_type： 杂志文章,已发布勘误

    doi：10.3390/v4102014

    authors： Mwimanzi P,Markle TJ,Ueno T,Brockman MA

    更新日期：2012-10-05 00:00:00

  • HHV-6A/B Integration and the Pathogenesis Associated with the Reactivation of Chromosomally Integrated HHV-6A/B.

    abstract：:Unlike other human herpesviruses, human herpesvirus 6A and 6B (HHV-6A/B) infection can lead to integration of the viral genome in human chromosomes. When integration occurs in germinal cells, the integrated HHV-6A/B genome can be transmitted to 50% of descendants. Such individuals, carrying one copy of the HHV-6A/B ge...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v9070160

    authors： Collin V,Flamand L

    更新日期：2017-06-26 00:00:00

  • Defining the Enterovirus Diversity Landscape of a Fecal Sample: A Methodological Challenge?

    abstract：:Enteroviruses are a group of over 250 naked icosahedral virus serotypes that have been associated with clinical conditions that range from intrauterine enterovirus transmission withfataloutcome through encephalitis and meningitis, to paralysis. Classically, enterovirus detection was done by assaying for the developmen...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8010018

    authors： Faleye TO,Adewumi MO,Adeniji JA

    更新日期：2016-01-12 00:00:00

  • Bioinformatics Pipeline for Human Papillomavirus Short Read Genomic Sequences Classification Using Support Vector Machine.

    abstract：:We recently developed a test based on the Agilent SureSelect target enrichment system capturing genomic fragments from 191 human papillomaviruses (HPV) types for Illumina sequencing. This enriched whole genome sequencing (eWGS) assay provides an approach to identify all HPV types in a sample. Here we present a machine...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12070710

    authors： Lomsadze A,Li T,Rajeevan MS,Unger ER,Borodovsky M

    更新日期：2020-06-30 00:00:00

  • Early Bunyavirus-Host Cell Interactions.

    abstract：:The Bunyaviridae is the largest family of RNA viruses, with over 350 members worldwide. Several of these viruses cause severe diseases in livestock and humans. With an increasing number and frequency of outbreaks, bunyaviruses represent a growing threat to public health and agricultural productivity globally. Yet, the...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8050143

    authors： Albornoz A,Hoffmann AB,Lozach PY,Tischler ND

    更新日期：2016-05-24 00:00:00

  • The Virus-Host Interplay: Biogenesis of +RNA Replication Complexes.

    abstract：:Positive-strand RNA (+RNA) viruses are an important group of human and animal pathogens that have significant global health and economic impacts. Notable members include West Nile virus, Dengue virus, Chikungunya, Severe acute respiratory syndrome (SARS) Coronavirus and enteroviruses of the Picornaviridae family.Unfor...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v7082825

    authors： Reid CR,Airo AM,Hobman TC

    更新日期：2015-08-06 00:00:00