Abstract：
BACKGROUND:Some children who have survived cancer will be azoospermic in the future. Performing isolation and purification procedures for spermatogonial stem cells (SSC) is very critical. In this regard, performing the process of decontamination of cancerous cells is the initial step. The major objective of the present study is to separate the malignant EL4 cell line in mice and spermatogonial stem cells in vitro. METHODS:The spermatogonial stem cells of sixty neonatal mice were isolated, and the procedure of co-culturing was carried out by EL4 which were classified into 2 major groups: (1) the control group (co-culture in a growth medium) and (2) the group of co-cultured cells which were separated using the microfluidic device. The percentage of cells was assessed using flow cytometry technique and common laboratory technique of immunocytochemistry and finally was confirmed through the laboratory technique of reverse transcription-polymerase chain reaction (RT-PCR). RESULTS:The actual percentage of EL4 and SSC after isolation was collected at two outlets: the outputs for the smaller outlet were 0.12% for SSC and 42.14% for EL4, while in the larger outlet, the outputs were 80.38% for SSC and 0.32% for EL4; in the control group, the percentages of cells were 21.44% for SSC and 23.28% for EL4 (based on t test (p ≤ 0.05)). CONCLUSIONS:The present study demonstrates that the use of the microfluidic device is effective in separating cancer cells from spermatogonial stem cells.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Ashtari B,Shams A,Esmaeilzadeh N,Tanbakooei S,Koruji M,Moghadam MJ,Ansari JM,Moghadam AJ,Shabani Rdoi
10.1186/s13287-020-01671-1subject
Has Abstractpub_date
2020-05-24 00:00:00pages
191issue
1issn
1757-6512pii
10.1186/s13287-020-01671-1journal_volume
11pub_type
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journal_title：Stem cell research & therapy
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pub_type： 评论,杂志文章
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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journal_title：Stem cell research & therapy
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