Molecular characteristics of Clostridium difficile in children with acute gastroenteritis from Zhejiang.

Abstract:

BACKGROUND:Clostridium difficile infection (CDI) has an increasing pediatric prevalence worldwide. However, molecular characteristics of C. difficile in Chinese children with acute gastroenteritis have not been reported. METHODS:A five-year cross-sectional study was conducted in a tertiary children's hospital in Zhejiang. Consecutive stool specimens from outpatient children with acute gastroenteritis were cultured for C. difficile, and isolates then were analyzed for toxin genes, multi-locus sequence type and antimicrobial resistance. Diarrhea-related viruses were detected, and demographic data were collected. RESULTS:A total of 115 CDI cases (14.3%), and 69 co-infected cases with both viruses and toxigenic C. difficile, were found in the 804 stool samples. The 186 C. difficile isolates included 6 of toxin A-positive/toxin B-positive/binary toxin-positive (A+B+CDT+), 139 of A+B+CDT-, 3 of A-B+CDT+, 36 of A-B+CDT- and 2 of A-B-CDT-. Sequence types 26 (17.7%), 35 (11.3%), 39 (12.4%), 54 (16.7%), and 152 (11.3%) were major genotypes with significant differences among different antimicrobial resistances (Fisher's exact test, P < 0.001). The A-B+ isolates had significantly higher resistance, compared to erythromycin, rifampin, moxifloxacin, and gatifloxacin, than that of the A+B+ (χ2 = 7.78 to 29.26, P < 0.01). The positive CDI rate in infants (16.2%) was significantly higher than that of children over 1 year old (10.8%) (χ2 = 4.39, P = 0.036). CONCLUSIONS:CDI has been revealed as a major cause of acute gastroenteritis in children with various genotypes. The role of toxigenic C. difficile and risk factors of CDI should be emphatically considered in subsequent diarrhea surveillance in children from China.

journal_name

BMC Infect Dis

journal_title

BMC infectious diseases

authors

Shuai H,Bian Q,Luo Y,Zhou X,Song X,Ye J,Huang Q,Peng Z,Wu J,Jiang J,Jin D

doi

10.1186/s12879-020-05030-6

subject

Has Abstract

pub_date

2020-05-13 00:00:00

pages

343

issue

1

issn

1471-2334

pii

10.1186/s12879-020-05030-6

journal_volume

20

pub_type

杂志文章
  • Decreasing trends, and geographical variation in outpatient antibiotic use: a population-based study in Central Denmark.

    abstract:BACKGROUND:Antimicrobial overuse and misuse of broad-spectrum antibiotics increases the risk for antimicrobial resistance. Investigating unwarranted variation in antibiotic prescription has therefore gained global priority. METHODS:We examined recent time trends in the utilization of narrow- and broad-spectrum antibio...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-019-3964-9

    authors: Kristensen PK,Johnsen SP,Thomsen RW

    更新日期:2019-04-24 00:00:00

  • Extended-spectrum beta-lactamase--producing enterobacteriaceae in the intensive care unit: acquisition does not mean cross-transmission.

    abstract:BACKGROUND:In intensive care unit (ICU), infection and colonization by resistant Gram-negative bacteria increase costs, length of stay and mortality. Extended-spectrum beta-lactamase--producing Enterobacteriaceae (ESBL-E) is a group of pathogens increasingly encountered in ICU setting. Conditions that promote ESBL-E ac...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-016-1489-z

    authors: Alves M,Lemire A,Decré D,Margetis D,Bigé N,Pichereau C,Ait-Oufella H,Baudel JL,Offenstadt G,Guidet B,Barbut F,Maury E

    更新日期:2016-04-13 00:00:00

  • Factors associated with sepsis development in 606 Spanish adult patients with cellulitis.

    abstract:BACKGROUND:Cellulitis, a frequent cause of admission of adult patients to medical wards, occasionally evolves to sepsis. In this study we analyze the factors related to sepsis development. METHODS:Prospective and observational study of 606 adult patients with cellulitis admitted to several Spanish hospitals. Comorbidi...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-020-4915-1

    authors: Collazos J,de la Fuente B,de la Fuente J,García A,Gómez H,Menéndez C,Enríquez H,Sánchez P,Alonso M,López-Cruz I,Martín-Regidor M,Martínez-Alonso A,Guerra J,Artero A,Blanes M,Asensi V

    更新日期:2020-03-12 00:00:00

  • Promoting routine syphilis screening among men who have sex with men in China: study protocol for a randomised controlled trial of syphilis self-testing and lottery incentive.

    abstract:BACKGROUND:Men who have sex with men (MSM) bear a high burden of syphilis infection. Expanding syphilis testing to improve timely diagnosis and treatment is critical to improve syphilis control. However, syphilis testing rates remain low among MSM, particularly in low- and middle-income countries. We describe the proto...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章,随机对照试验

    doi:10.1186/s12879-020-05188-z

    authors: Cheng W,Wang C,Tang W,Ong JJ,Fu H,Marks M,Smith MK,Li C,Nie J,Zhao P,Zheng H,Yang B,Tucker JD

    更新日期:2020-06-29 00:00:00

  • Surveillance and characterisation of influenza viruses among patients with influenza-like illness in Bali, Indonesia, July 2010-June 2014.

    abstract:BACKGROUND:Although Indonesia has high fatality rate of human A/H5N1 cases, epidemiological and clinical data on influenza virus circulation among humans has been limited. Within Indonesia, Bali province is of interest due to high population densities of humans, pigs and poultry. This study aims to characterize and com...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-019-3842-5

    authors: Adisasmito W,Budayanti S,Aisyah DN,Coker R,Andayani AR,Smith GJD,Rudge JW

    更新日期:2019-03-07 00:00:00

  • Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China.

    abstract:BACKGROUND:Variations of porB1A and porB1B genes and their serotypes exist in Neisseria gonorrhoeae isolates from different geographical areas, and some site mutations in the porB1B gene correlate with drug resistance. METHODS:The β-lactamase production of N. gonorrhoeae isolates was determined by paper acidometric te...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-323

    authors: Sun A,Fan X,Gu Y,Du P,Tang R,Mao Y,Lin X,Yan J

    更新日期:2010-11-10 00:00:00

  • Knowledge, attitudes and behaviour of hospital health-care workers regarding influenza A/H1N1: a cross sectional survey.

    abstract:BACKGROUND:To assess the knowledge, the attitudes, and the behaviour towards influenza A/H1N1 and the vaccination among health-care workers (HCWs). METHODS:A sample of HCWs was selected from a random sample of non-teaching public hospitals, located in the cities of Naples and Avellino (Italy), received a self-administ...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-14-208

    authors: Albano L,Matuozzo A,Marinelli P,Di Giuseppe G

    更新日期:2014-04-16 00:00:00

  • Blood borne transit of CJD from brain to gut at early stages of infection.

    abstract:BACKGROUND:In Creutzfeldt-Jakob disease (CJD) and other related transmissible spongiform encephalopathies it is critical to understand the various pathways by which the infectious agent spreads to different organs. METHODS:We injected a CJD agent into mice, either intracerebrally (ic) or intraperitoneally (ip) and mon...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-1-20

    authors: Radebold K,Chernyak M,Martin D,Manuelidis L

    更新日期:2001-01-01 00:00:00

  • Clinical characteristics and managements of severe hand, foot and mouth disease caused by enterovirus A71 and coxsackievirus A16 in Shanghai, China.

    abstract:BACKGROUND:Hand, foot and mouth disease (HFMD) is a transmissible infectious disease caused by human enteroviruses (EV). Here, we described features of children with severe HFMD caused by EV-A71 or coxsackievirus A16 (CV-A16) in Shanghai, China. METHODS:Severe EV-A71 or CV-A16 caused HFMD children admitted to the Xinh...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-019-3878-6

    authors: Cai K,Wang Y,Guo Z,Yu H,Li H,Zhang L,Xu S,Zhang Q

    更新日期:2019-03-27 00:00:00

  • Safety and efficacy of high-dose daptomycin as salvage therapy for severe gram-positive bacterial sepsis in hospitalized adult patients.

    abstract:BACKGROUND:Increasing the dosage of daptomycin may be advantageous in severe infection by enhancing bactericidal activity and pharmacodynamics. However, clinical data on using daptomycin at doses above 6 mg/kg in Asian population are limited. METHODS:A retrospective observational cohort study of all hospitalized adult...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-13-66

    authors: Lai CC,Sheng WH,Wang JT,Cheng A,Chuang YC,Chen YC,Chang SC

    更新日期:2013-02-04 00:00:00

  • The global effect of maternal education on complete childhood vaccination: a systematic review and meta-analysis.

    abstract:BACKGROUND:There is an established correlation between maternal education and reduction in childhood mortality. One proposed link is that an increase in maternal education will lead to an increase in health care access and vaccine uptake. Vaccinations are a central preventative child health tool, therefore demonstratin...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/s12879-017-2890-y

    authors: Forshaw J,Gerver SM,Gill M,Cooper E,Manikam L,Ward H

    更新日期:2017-12-28 00:00:00

  • Epidemiology and risk factors for Staphylococcus aureus colonization in children in the post-PCV7 era.

    abstract:BACKGROUND:The incidence of community-associated methicillin-resistant Staphylococcus aureus (MRSA) has risen dramatically in the U.S., particularly among children. Although Streptococcus pneumoniae colonization has been inversely associated with S. aureus colonization in unvaccinated children, this and other risk fact...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-9-110

    authors: Lee GM,Huang SS,Rifas-Shiman SL,Hinrichsen VL,Pelton SI,Kleinman K,Hanage WP,Lipsitch M,McAdam AJ,Finkelstein JA

    更新日期:2009-07-11 00:00:00

  • Tuberculous meningitis is a major cause of mortality and morbidity in adults with central nervous system infections in Kota Kinabalu, Sabah, Malaysia: an observational study.

    abstract:BACKGROUND:Central nervous system (CNS) infections are a significant contributor to morbidity and mortality globally. However, most published studies have been conducted in developed countries where the epidemiology and aetiology differ significantly from less developed areas. Additionally, there may be regional differ...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-016-1640-x

    authors: Lee HG,William T,Menon J,Ralph AP,Ooi EE,Hou Y,Sessions O,Yeo TW

    更新日期:2016-06-16 00:00:00

  • Viral aetiology of bronchiolitis in hospitalised children in Qatar.

    abstract:BACKGROUND:Bronchiolitis is considered one of the earliest and most common causes of hospitalisation in young children. Development of molecular technologies allowed a better understanding of bronchiolitis aetiology. Results from cohort studies evaluating the association between single, multiple viral infections and cl...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-017-2225-z

    authors: Janahi I,Abdulkayoum A,Almeshwesh F,Alkuwari M,Al Hammadi A,Alameri M

    更新日期:2017-02-13 00:00:00

  • Clinical and imaging findings of discharged patients with SARS-CoV-2 positive anal swab samples: a descriptive study.

    abstract:BACKGROUND:To explore the clinical features and CT findings of clinically cured coronavirus disease 2019 (COVID-19) patients with viral RNA positive anal swab results after discharge. METHODS:Forty-two patients with COVID-19 who were admitted to Yongzhou Central Hospital, Hunan, China, between January 20, 2020, and Ma...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-020-05363-2

    authors: Zhou H,Zhu L,Leng Y,Wang D,Bai HX,Xiong Z,Shi L,Liao W

    更新日期:2020-09-01 00:00:00

  • Precision diagnosis and therapy of a case of brain abscesses associated with asymptomatic pulmonary arteriovenous fistulas.

    abstract:BACKGROUND:Brain abscesses, a severe infectious disease of the CNS, are usually caused by a variety of different pathogens, which include Streptococcus intermedius (S. intermedius). Pulmonary arteriovenous fistulas (PAVFs), characterized by abnormal direct communication between pulmonary artery and vein, are a rare und...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-020-05092-6

    authors: Gao LY,Xu GR,Dai TJ

    更新日期:2020-05-24 00:00:00

  • Detection of varicella-zoster virus from cerebrospinal fluid using advanced fragment analysis in a child with encephalitis: a case report.

    abstract:BACKGROUND:Varicella zoster virus (VZV) encephalitis is an infectious inflammatory disease of brain that can cause irreversible mental damage without timely treatment. In fact, many viruses can cause encephalitis, and the viral loads in cerebrospinal fluid (CSF) in the early stage of the disease are usually too low to ...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-019-3986-3

    authors: Song YX,Li Y,Jiang YM,Liu T

    更新日期:2019-04-25 00:00:00

  • C-reactive protein serum levels as an early predictor of outcome in patients with pandemic H1N1 influenza A virus infection.

    abstract:BACKGROUND:Data for predicting which patients with pandemic influenza A (H1N1) infection are likely to run a complicated course are sparse. We retrospectively studied whether the admission serum C-reactive protein (CRP) levels can serve as a predictor of illness severity. METHODS:Included were all consecutive adult pa...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-288

    authors: Zimmerman O,Rogowski O,Aviram G,Mizrahi M,Zeltser D,Justo D,Dahan E,Arad R,Touvia O,Tau L,Tarabeia J,Berliner S,Paran Y

    更新日期:2010-10-04 00:00:00

  • Clinical characteristics of hospital-onset Pneumocystis pneumonia and genotypes of Pneumocystis jirovecii in a single tertiary centre in Korea.

    abstract:BACKGROUND:Pneumocystis pneumonia (PCP) may develop as a clinical manifestation of nosocomial pneumonia by means of either reactivation of resident P. jirovecii or de novo infection. However, there have been no studies describing the clinical characteristics of hospital-onset PCP. METHODS:A retrospective review of med...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-015-0847-6

    authors: Kim T,Lee SO,Hong HL,Lee JY,Kim SH,Choi SH,Kim MN,Kim YS,Woo JH,Sung H

    更新日期:2015-02-26 00:00:00

  • Incidence and determinants of new AIDS-defining illnesses after HAART initiation in a Senegalese cohort.

    abstract:BACKGROUND:Although a dramatic decrease in AIDS progression has been observed after Highly Active Anti Retroviral Therapy (HAART) in both low- and high-resource settings, few data support that fact in low-resource settings.This study describes the incidence of AIDS-defining illnesses (ADI) after HAART initiation and an...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-179

    authors: De Beaudrap P,Etard JF,Diouf A,Ndiaye I,Ndèye GF,Sow PS,Ndèye KC,Ecochard R,Delaporte E,ANRS 1215 Study Group.

    更新日期:2010-06-19 00:00:00

  • Prevalence and risk factors for significant liver fibrosis among HIV-monoinfected patients.

    abstract:BACKGROUND:HIV-monoinfected patients may be at risk for significant liver fibrosis, but its prevalence and determinants in these patients are unknown. Since HIV-monoinfected patients do not routinely undergo liver biopsy, we evaluated the prevalence and risk factors of significant hepatic fibrosis in this group using t...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-116

    authors: DallaPiazza M,Amorosa VK,Localio R,Kostman JR,Lo Re V 3rd

    更新日期:2010-05-13 00:00:00

  • The realistic performance achievable with mycobacterial automated culture systems in high and low prevalence settings.

    abstract:BACKGROUND:Diagnostic tests are generally used in situations with similar pre-test probability of disease to where they were developed. When these tests are applied in situations with very different pre-test probabilities of disease, it is informative to model the likely implications of known characteristics of test pe...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-93

    authors: van Kampen SC,Anthony RM,Klatser PR

    更新日期:2010-04-12 00:00:00

  • Disseminated Saksenaea infection in an immunocompromised host associated with a good clinical outcome: a case report and review of the literature.

    abstract:BACKGROUND:Saksenaea species (spp.) are uncommon causes of mucormycosis but are emerging pathogens mostly associated with trauma and soil contamination often in immunocompetent hosts. Due to lack of sporulation in the laboratory, diagnosis and susceptibility testing is difficult so optimal treatment regimens are unknow...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章,评审

    doi:10.1186/s12879-020-05459-9

    authors: Davidson N,Campbell K,Foroughi F,Tayal V,Lynar S,Crawford LC,Kidd SE,Baird R,Davies J,Meumann EM

    更新日期:2020-10-14 00:00:00

  • Solidarity, vulnerability and mistrust: how context, information and government affect the lives of women in times of Zika.

    abstract:BACKGROUND:The public health response to Zika outbreak has mostly focused on epidemiological surveillance, vector control, and individual level preventative measures. This qualitative study employs a social-ecological framework to examine how macro (historical, legislative, political, socio-economic factors), meso (sou...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-020-04987-8

    authors: Linde-Arias AR,Roura M,Siqueira E

    更新日期:2020-04-03 00:00:00

  • Estimation of HPV prevalence in young women in Scotland; monitoring of future vaccine impact.

    abstract:BACKGROUND:Estimation of pre-immunisation prevalence of HPV and distribution of HPV types is fundamental to understanding the subsequent impact of HPV vaccination. We describe the type specific prevalence of HPV in females aged 20-21 in Scotland who attended or defaulted from cervical screening using three specimen typ...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-13-519

    authors: Kavanagh K,Sinka K,Cuschieri K,Love J,Potts A,Pollock KG,Cubie H,Donaghy M,Robertson C

    更新日期:2013-11-05 00:00:00

  • Molecular detection of fluoroquinolone-resistance in multi-drug resistant tuberculosis in Cambodia suggests low association with XDR phenotypes.

    abstract:BACKGROUND:Drug susceptibility testing (DST) remains an important concern for implementing treatment of MDR tuberculosis patients. Implementation of molecular tests for drug resistance identification would facilitate DST particularly in developing countries where culturing is difficult to perform. We have characterized...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-11-255

    authors: Surcouf C,Heng S,Pierre-Audigier C,Cadet-Daniel V,Namouchi A,Murray A,Gicquel B,Guillard B

    更新日期:2011-09-28 00:00:00

  • Development of tissue inflammation accompanied by NLRP3 inflammasome activation in rabbits infected with Treponema pallidum strain Nichols.

    abstract:BACKGROUND:The inflammasome responses in Treponema pallidum infection have been poorly understood to date. This study aimed to investigate the expression of the nucleotide-binding leucine-rich receptor protein 3 (NLRP3) inflammasome in the development of tissue inflammation in rabbits infected with T. pallidum. METHOD...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/s12879-018-2993-0

    authors: Lin LR,Xiao Y,Liu W,Chen YY,Zhu XZ,Gao ZX,Gao K,Tong ML,Zhang HL,Li SL,Lin HL,Li WD,Liang XM,Lin Y,Liu LL,Yang TC

    更新日期:2018-03-01 00:00:00

  • Application of quantitative second-line drug susceptibility testing at a multidrug-resistant tuberculosis hospital in Tanzania.

    abstract:BACKGROUND:Lack of rapid and reliable susceptibility testing for second-line drugs used in the treatment of multidrug-resistant tuberculosis (MDR-TB) may limit treatment success. METHODS:Mycobacterium tuberculosis isolates from patients referred to Kibong'oto National TB Hospital in Tanzania for second-line TB treatme...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-13-432

    authors: Mpagama SG,Houpt ER,Stroup S,Kumburu H,Gratz J,Kibiki GS,Heysell SK

    更新日期:2013-09-14 00:00:00

  • The use of a geographic information system to identify a dairy goat farm as the most likely source of an urban Q-fever outbreak.

    abstract:BACKGROUND:A Q-fever outbreak occurred in an urban area in the south of the Netherlands in May 2008. The distribution and timing of cases suggested a common source. We studied the spatial relationship between the residence locations of human cases and nearby small ruminant farms, of which one dairy goat farm had experi...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-10-69

    authors: Schimmer B,Ter Schegget R,Wegdam M,Züchner L,de Bruin A,Schneeberger PM,Veenstra T,Vellema P,van der Hoek W

    更新日期:2010-03-16 00:00:00

  • Stable hepatitis C virus RNA detection by RT-PCR during four days storage.

    abstract:BACKGROUND:Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room t...

    journal_title:BMC infectious diseases

    pub_type: 杂志文章

    doi:10.1186/1471-2334-2-22

    authors: de Moreau de Gerbehaye AI,Bodéus M,Robert A,Horsmans Y,Goubau P

    更新日期:2002-10-04 00:00:00