Abstract:
OBJECTIVES:Non-alcoholic fatty liver disease (NAFLD) is considered a well-known pathology that is determined without using alcohol and has emerged as a growing public health problem. Lipotoxicity is known to promote hepatocyte death, which, in the context of NAFLD, is termed lipoapoptosis. The severity of NAFLD correlates with the degree of hepatocyte lipoapoptosis. Protein-tyrosine phosphatases (PTP) including PTP1B and Low molecular weight PTP (LMPTP), are negative regulators of the insulin signaling pathway and are considered a promising therapeutic target in the treatment of diabetes. In this study, we hypothesized that the inhibition of PTP1B and LMPTP may potentially prevent hepatocyte apoptosis, mitochondrial dysfunction and endoplasmic reticulum (ER) stress onset, following lipotoxicity induced using a free fatty acid (FFA) mixture. METHODS:HepG2 cells were cultured in the presence or absence of two PTP inhibitors, namely MSI-1436 and Compound 23, prior to palmitate/oleate overloading. Apoptosis, ER stress, oxidative stress, and mitochondrial dynamics were then evaluated by either MUSE or RT-qPCR analysis. RESULTS:The obtained data demonstrate that the inhibition of PTP1B and LMPTP prevents apoptosis induced by palmitate and oleate in the HepG2 cell line. Moreover, mitochondrial dynamics were positively improved following inhibition of the enzyme, with concomitant oxidative stress reduction and ER stress abrogation. CONCLUSION:In conclusion, PTP's inhibitory properties may be a promising therapeutic strategy for the treatment of FFA-induced lipotoxicity in the liver and ultimately in the management of the NAFLD condition.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Bourebaba L,Łyczko J,Alicka M,Bourebaba N,Szumny A,Fal AM,Marycz Kdoi
10.3390/jcm9051294subject
Has Abstractpub_date
2020-05-01 00:00:00issue
5issn
2077-0383pii
jcm9051294journal_volume
9pub_type
杂志文章abstract::Limited information is available regarding interstitial lung disease (ILD) in Erdheim⁻Chester disease (ECD), a rare multisystemic non-Langerhans cell histiocytosis. Sixty-two biopsy-confirmed ECD patients were divided into those with no ILD (19.5%), minimal ILD (32%), mild ILD (29%), and moderate/severe ILD (19.5%), b...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm7090243
更新日期:2018-08-28 00:00:00
abstract::Allogeneic stem cell transplantation (allo-SCT) is the preferred curative treatment for several hematological malignancies. The efficacy of allo-SCT depends on the graft-versus-leukemia (GvL) effect. However, the prognosis of patients with relapsed acute myeloid leukemia (AML) following allo-SCT is poor. Donor lymphoc...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9010039
更新日期:2019-12-23 00:00:00
abstract:PURPOSE:To investigate the value of visual acuity and patient-perceived visual function test when subretinal drusenoid deposits (SDD) are incorporated into the classification of age-related macular degeneration (AMD). A total of 50 participants were recruited into the study in these groups: healthy ageing (n = 11), int...
journal_title:Journal of clinical medicine
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doi:10.3390/jcm9092832
更新日期:2020-09-01 00:00:00
abstract::Previous studies have shown better clinical outcomes after total hip arthroplasty (THA) compared to hemiarthroplasty (HA) for displaced femoral neck fracture. However, few studies have focused on the surgical risks of the two procedures. Therefore, we investigated the perioperative complications of HA and THA in femor...
journal_title:Journal of clinical medicine
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doi:10.3390/jcm9103203
更新日期:2020-10-03 00:00:00
abstract::Photodynamic therapy (PDT) is an anticancer strategy utilizing light-mediated activation of a photosensitizer (PS) which has accumulated in tumor and/or surrounding vasculature. Upon activation, the PS mediates tumor destruction through the generation of reactive oxygen species and tumor-associated vasculature damage,...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9020333
更新日期:2020-01-24 00:00:00
abstract::Acute retinal necrosis (ARN) is a rare viral endophthalmitis, and human herpesvirus is the principal pathogen. Early diagnosis and treatment are critical to avoid visual impairment by ARN, and pars plana vitrectomy (PPV) is required in advanced cases. In this study, we evaluated the transition of viral load in ocular ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9041204
更新日期:2020-04-22 00:00:00
abstract::Endoscopic resection has been the standard treatment for intramucosal esophageal cancers (ECs) because of the low risk of lymph node metastases in the lesions. In recent years, endoscopic submucosal dissection (ESD), which can resect large ECs, has been performed. However, the risk of esophageal stricture after ESD is...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm10010020
更新日期:2020-12-23 00:00:00
abstract:BACKGROUND:Thyroid hormone modulation of cardiovascular function has been associated with cardiovascular disease. Recent evidence suggests that free thyroxine (FT4) levels are associated with an increase in systemic arterial stiffness, but little is known about the effects of FT4 at the local level of the common caroti...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm10030420
更新日期:2021-01-22 00:00:00
abstract::This study aimed to develop a prognosis-predicting model based on [18F]fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) and clinicopathologic factors in locally advanced cervical cancer patients treated with concurrent chemoradiotherapy (CCRT). The medical records of 270 locally adv...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9020427
更新日期:2020-02-05 00:00:00
abstract::Children are susceptible to a variety of respiratory infections. Wheezing is a common sign presented by children with respiratory infections. Asthma, bronchiolitis, and bronchitis are common causes of childhood wheezing disease (CWD) and are regarded as overlapping disease spectra. Macrolides are common antimicrobial ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm7110432
更新日期:2018-11-09 00:00:00
abstract:BACKGROUND:Oral and enteral mucositis due to high-dose cytostatic treatment administered during autologous and allogeneic stem-cell transplantation increases mortality. Salivary secretory immunoglobulin A (sIgA) is a basic pillar of local immunity in the first line of defense. Altered salivary sialoglycoprotein carbohy...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9061747
更新日期:2020-06-05 00:00:00
abstract::Several conditions can lead to the development of a subchondral cyst. The mechanism by which the cysts form, their location, and their severity depend on the underlying pathology, although the exact pathogenesis is not fully elucidated. Treatment options vary according to the location of the cyst, with less invasive p...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9051358
更新日期:2020-05-06 00:00:00
abstract::The aim of this systematic review and meta-analysis was to evaluate the clinical performance of tooth-borne partial and full-coverage fixed dental prosthesis fabricated using hybrid polymer and ceramic CAD/CAM materials regarding their biologic, technical and esthetical outcomes. PICOS search strategy was applied usin...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9072107
更新日期:2020-07-04 00:00:00
abstract::Low skeletal muscle mass (LSMM) is increasingly recognized for its predictive value for adverse events in cancer patients. In specific, the predictive value of LSMM has been demonstrated for anti-cancer drug toxicity in a variety of cancer types and anti-cancer drugs. However, due to the limited sample size and study ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9113780
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:We investigated the associations between v-Raf murine sarcoma viral oncogene homolog B1 (BRAFV600E, henceforth BRAF) and v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations and colorectal cancer (CRC) prognosis, using The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GSE39582)...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8010111
更新日期:2019-01-17 00:00:00
abstract:BACKGROUND:The Glasgow-Blatchford Score (GBS) is one of the most widely used scoring systems for predicting clinical outcomes for gastrointestinal bleeding (GIB). However, the clinical significance of the GBS in predicting GIB in patients with heart failure (HF) remains unclear. METHODS AND RESULTS:We conducted a pros...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9124083
更新日期:2020-12-17 00:00:00
abstract::Treatment with immune checkpoint inhibitors has shown efficacy against a variety of cancer types. The effects of nivolumab and pembrolizumab on lung cancer have been reported, and further therapeutic advances are ongoing. The side effects of immune checkpoint inhibitors are very different from those of conventional cy...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9072033
更新日期:2020-06-29 00:00:00
abstract::Despite the overall success of cochlear implantation, language outcomes remain suboptimal and subject to large inter-individual variability. Early auditory rehabilitation techniques have mostly focused on low-level sensory abilities. However, a new body of literature suggests that cognitive operations are critical for...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9041058
更新日期:2020-04-08 00:00:00
abstract::Background: We decided to investigate whether aneurysm wall enhancement (AWE) on high-resolution vessel wall magnetic resonance imaging (HR VW-MRI) coexists with the conventional risk factors for aneurysm rupture. Methods: We performed HR VW-MRI in 46 patients with 64 unruptured small intracranial aneurysms. Patient d...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm10020225
更新日期:2021-01-10 00:00:00
abstract:BACKGROUND:No dural substitute has proven to be complication-free in a large clinical trial, even suggesting some benefit during watertight closure. However, primary dural closure is not always possible due to dural shrinkage from electrocautery for dural bleeding. OBJECTIVE:This study is performed to analyze the clin...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9040902
更新日期:2020-03-25 00:00:00
abstract::Acute kidney injury (AKI) is characterized by an acute decrease in renal function that can be multifactorial in its origin and is associated with complex pathophysiological mechanisms. In the short term, AKI is associated with an increased length of hospital stay, health care costs, and in-hospital mortality, and its ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章,评审
doi:10.3390/jcm9061704
更新日期:2020-06-02 00:00:00
abstract::Our aim was to illustrate our technique of sex-sparing (SS)-robot-assisted radical cystectomy (RARC) in female patients receiving an intracorporeal neobladder (iN). From January 2013 to June 2018, 11 female patients underwent SS-RARC-iN at a single tertiary referral center. Inclusion criteria were a cT ≤ 2 N0 M0 bladd...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9020577
更新日期:2020-02-20 00:00:00
abstract::The detection of rare mutational targets in plasma (liquid biopsy) has emerged as a promising tool for the assessment of patients with cancer. We determined the presence of cell-free DNA containing the BRAFV600E mutations (cfBRAFV600E) in plasma samples from 57 patients with papillary thyroid cancer (PTC) with somatic...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9082481
更新日期:2020-08-02 00:00:00
abstract::The aim of this study was to assess if women with a low first trimester maternal pregnancy-associated plasma protein-A (PAPP-A) level are at increased risk of emergency cesarean (EmCS) for intrapartum fetal compromise (IFC) and/or adverse neonatal outcomes. This was a retrospective cohort study performed at Mater Moth...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9041108
更新日期:2020-04-13 00:00:00
abstract::The geographic spread of 2019 novel coronavirus (COVID-19) infections from the epicenter of Wuhan, China, has provided an opportunity to study the natural history of the recently emerged virus. Using publicly available event-date data from the ongoing epidemic, the present study investigated the incubation period and ...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9020538
更新日期:2020-02-17 00:00:00
abstract::The purpose of this study was to investigate the effect of high molecular weight hyaluronan (HMWHA) eye drops on subbasal corneal nerves in patients suffering from severe dry eye disease (DED) and to evaluate the damage of subbasal corneal nerves associated with severe DED. Designed as an international, multicenter st...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9123799
更新日期:2020-11-24 00:00:00
abstract::Pulmonary hypertension (PH) in patients with heart failure (HF) contributes to a poorer prognosis. However, in those with dilated cardiomyopathy (DCM), the true prevalence and role of PH is unclear. Therefore, this study aimed to analyze the profile of DCM patients at various levels of PH risk, determined via echocard...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9061660
更新日期:2020-06-01 00:00:00
abstract::The prognosis of multiple myeloma (MM) has improved with the introduction of novel agents. These data are largely derived from clinical trials and might not reflect real-world patient outcomes accurately. We surveyed real-world data from 284 patients newly diagnosed with MM between 2010 and 2018 in Miyazaki Prefecture...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm10010105
更新日期:2020-12-30 00:00:00
abstract:INTRODUCTION:Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are an increasing tumor entity. Since many patients are diagnosed at an advanced stage, treatment is still challenging and dependent on many tumor and patient specific factors. Therefore, the aim of the present study was to elucidate the expression...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm9103368
更新日期:2020-10-21 00:00:00
abstract::Stress response to robot-assisted colorectal surgery is largely unknown. Therefore, we conducted a prospective comparative nonrandomized study evaluating the perioperative dynamics of chemokines: IL-8/CXCL8, MCP-1/CCL2, MIP-1α/CCL3, MIP-1β/CCL4, RANTES/CCL5, and eotaxin-1/CCL11 in 61 colorectal cancer patients followi...
journal_title:Journal of clinical medicine
pub_type: 杂志文章
doi:10.3390/jcm8060879
更新日期:2019-06-19 00:00:00