当前位置： SCI文献检索 > BMC CANCER期刊下所有文献 > Mutation analysis by deep sequencing of pancreatic juice from patients with pancreatic ductal adenocarcinoma.

Mutation analysis by deep sequencing of pancreatic juice from patients with pancreatic ductal adenocarcinoma.

Abstract：

BACKGROUND:Reliable methods are needed to identify patients with early-stage cancer or high-grade precancerous lesions in the pancreas. Analysis of pancreatic juice to detect somatic mutations could represent one such approach. Here we investigated the concordance between mutations found in the primary tumor and pancreatic juice from the same patient. METHODS:Amplicon-based targeted deep sequencing was performed on samples from 21 patients with pancreatic ductal adenocarcinoma (PDAC) who had undergone Whipple's operation. Mutation profiles were determined in formalin-fixed sections of the primary tumor and in pancreatic juice sampled from the main pancreatic duct during surgery. RESULTS:Using a cut-off of 3% for variant allele frequency, KRAS mutations were detected in 20/21 primary tumors (95%) and in 15/21 (71%) juice samples. When also considering low-frequency variants, KRAS mutations were found in 20/21 juice samples. Most juice samples exhibited multiple KRAS variants not seen in the primary tumor, and only in 11 cases (52%) did the most abundant variant of the juice correspond to the KRAS mutation detected in the tumor. TP53 mutations were found in 16 tumors (76%) and six juice samples (29%). Among the positive juice samples, only one exhibited more than a single TP53 mutation. Detection of both KRAS and TP53 mutations was fully concordant in the primary tumor and juice sample in 7/21 cases (33%). CONCLUSIONS:Pancreatic juice from PDAC patients is rich in KRAS mutations often not seen in the primary tumor and possibly reflecting precancerous lesions in other regions of the pancreas. The inclusion of TP53 mutation detection and additional markers must therefore be considered for fully exploiting the clinical potential of pancreatic juice samples in early cancer detection.

journal_name

BMC Cancer

journal_title

BMC cancer

authors

Choi MH,Mejlænder-Andersen E,Manueldas S,El Jellas K,Steine SJ,Tjensvoll K,Sætran HA,Knappskog S,Hoem D,Nordgård O,Hovland R,Molven A

doi

10.1186/s12885-018-5195-7

subject

Has Abstract

pub_date

2019-01-05 00:00:00

pages

issue

issn

1471-2407

pii

10.1186/s12885-018-5195-7

journal_volume

pub_type

杂志文章

在线工具

Mutation analysis by deep sequencing of pancreatic juice from patients with pancreatic ductal adenocarcinoma.