Abstract:
BACKGROUND:Aberrant expression of microRNAs in different human cancer types has been widely reported. MiR-218 acts as a tumor suppressor in diverse human cancer types impacting regulation of multiple genes in oncogenic pathways. Here, we evaluated the expression and function of miR-218 in human lung cancer and ALDH positive lung cancer cells to understand the potential mechanisms responsible for disease pathology. Also, the association between its host genes and the target genes could be useful towards the better understanding of prognosis in clinical settings. METHODS:Publicly-available data from The Cancer Genome Atlas (TCGA) was mined to compare the levels of miR-218 and its host gene SLIT2/3 between lung cancer tissues and normal lung tissues. Transfection of miR-218 to investigate its function in lung cancer cells was done and in vivo effects were determined using miR-218 expressing lentiviruses. Aldefluor assay and Flow cytometry was used to quantify and enrich ALDH positive lung cancer cells. Levels of miR-218, IL-6R, JAK3 and phosphorylated STAT3 were compared in ALDH1A1 positive and ALDH1A1 negative cells. Overexpression of miR-218 in ALDH positive cells was carried to test the survival by tumorsphere culture. Finally, utilizing TCGA data we studied the association of target genes of miR-218 with the prognosis of lung cancer. RESULTS:We observed that the expression of miR-218 was significantly down-regulated in lung cancer tissues compared to normal lung tissues. Overexpression of miR-218 decreased cell proliferation, invasion, colony formation, and tumor sphere formation in vitro and repressed tumor growth in vivo. We further found that miR-218 negatively regulated IL-6 receptor and JAK3 gene expression by directly targeting the 3'-UTR of their mRNAs. In addition, the levels of both miR-218 host genes and the components of IL-6/STAT3 pathway correlated with prognosis of lung cancer patients. CONCLUSIONS:MiR-218 acts as a tumor suppressor in lung cancer via IL-6/STAT3 signaling pathway regulation.
journal_name
Mol Cancerjournal_title
Molecular cancerauthors
Yang Y,Ding L,Hu Q,Xia J,Sun J,Wang X,Xiong H,Gurbani D,Li L,Liu Y,Liu Adoi
10.1186/s12943-017-0710-zsubject
Has Abstractpub_date
2017-08-22 00:00:00pages
141issue
1issn
1476-4598pii
10.1186/s12943-017-0710-zjournal_volume
16pub_type
杂志文章相关文献
Molecular Cancer文献大全abstract:BACKGROUND:The Ras-association family (RASSF) of tumour suppressor genes (TSGs) contains 10 members that encode proteins containing Ras-association (RA) domains. Several members of the RASSF family are frequently epigenetically inactivated in cancer, however, their role in leukaemia has remained largely uninvestigated....
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-42
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:The human cell cycle transcription factor FOXM1 is known to play a key role in regulating timely mitotic progression and accurate chromosomal segregation during cell division. Deregulation of FOXM1 has been linked to a majority of human cancers. We previously showed that FOXM1 was upregulated in basal cell c...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-45
更新日期:2010-02-26 00:00:00
abstract::Several studies of tumors have revealed substantial numbers of clonally expanded somatic mutations in mitochondrial DNA (mtDNA), not observed in adjacent intact tissues. These findings were interpreted as indicating the involvement of mtDNA mutations in tumorigenesis. Such comparisons, however, ignore an important con...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/1476-4598-4-36
更新日期:2005-09-13 00:00:00
abstract:BACKGROUND:Granulosa cell tumors (GCTs) are relatively rare and are subtypes of the sex-cord stromal neoplasms. Methylation induced silencing in the promoters of genes such as tumor suppressor genes, DNA repair genes and pro-apoptotic genes is recognised as a critical factor in cancer development. METHODS:We examined ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-3-33
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND:Hodgkin lymphoma (HL) and Anaplastic Large Cell Lymphoma (ALCL), are forms of malignant lymphoma defined by unique morphologic, immunophenotypic, genotypic, and clinical characteristics, but both overexpress CD30. We used sub-megabase resolution tiling (SMRT) array-based comparative genomic hybridization to ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-7-2
更新日期:2008-01-07 00:00:00
abstract:BACKGROUND:Increasing evidence supports the role of small nucleolar RNAs (snoRNAs) and long non-coding RNAs (lncRNAs) as master gene regulators at the epigenetic modification level. However, the underlying mechanism of these functional ncRNAs in colorectal cancer (CRC) has not been well investigated. METHODS:The dysre...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01201-w
更新日期:2020-05-22 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Molecular cancer
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12943-020-01252-z
更新日期:2020-09-09 00:00:00
abstract:BACKGROUND:Platinum-containing chemotherapy produces specific DNA damage and is used to treat several human solid tumors. Tumors initially sensitive to platinum-based drugs frequently become resistant. Inhibition of DNA repair is a potential strategy to enhance cisplatin effectiveness. After cisplatin treatment, a bala...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-10-74
更新日期:2011-06-16 00:00:00
abstract:BACKGROUND:RNA interference (RNAi), a newly developed method in which RNA molecules inhibit gene expression, has recently received considerable research attention. In the development of RNAi-based therapies, nanoparticles, which have distinctive size effects along with facile modification strategies and are capable of ...
journal_title:Molecular cancer
pub_type: 杂志文章,评审
doi:10.1186/s12943-017-0683-y
更新日期:2017-07-28 00:00:00
abstract::The mammalian target of rapamycin (mTOR), which exists in two functionally distinct complexes, mTORC1 and mTORC2 plays an important role in tumor growth. Whereas the role of mTORC1 has been well characterized in this process, little is known about the functions of mTORC2 in cancer progression. In this study, we explor...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-57
更新日期:2010-03-12 00:00:00
abstract:BACKGROUND:Survivin is a dual function protein. It inhibits the apoptosis of cells by inhibiting caspases, and also promotes cell growth by stabilizing microtubules during mitosis. Over-expression of survivin has been demonstrated to induce drug-resistance to various chemo-therapeutic agents such as cisplatin (DNA dama...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-43
更新日期:2009-07-03 00:00:00
abstract:BACKGROUND:TPC-1 is a papillary thyroid carcinoma (PTC)-derived cell line that spontaneously expresses the oncogene RET/PTC1. TPC-1 treated with the RET/PTC1 inhibitor RPI-1 displayed a cytostatic and reversible inhibition of cell proliferation and a strong activation of focal adhesion kinase (FAK). As dasatinib inhibi...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-278
更新日期:2010-10-18 00:00:00
abstract::Since the publication of this work [1] and in response to a recent query that was brought to our attention in relation to the Western Blot in Figure 1(C) for NP2, protein lysates prepared around the same time as those presented in the manuscript in question, were run by SDS-PAGE under similar experimental conditions a...
journal_title:Molecular cancer
pub_type: 已发布勘误
doi:10.1186/s12943-020-1142-8
更新日期:2020-01-27 00:00:00
abstract:BACKGROUND:In addition to androgens, growth factors are also implicated in the development and neoplastic growth of the prostate gland. Prosaposin is a potent neurotrophic molecule. Homozygous inactivation of prosaposin in mice has led to the development of a number of abnormalities in the male reproductive system, inc...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-3-31
更新日期:2004-11-17 00:00:00
abstract:BACKGROUND:Non-small cell lung cancer (NSCLC) is the number one cancer killer. Tumor-initiating cells (TICs) are responsible for tumor progression and recurrence. Emerging evidences suggest that small nucleolar RNAs (snoRNAs) play malfunctioning roles in lung tumorigenesis. This study aims to determine if snoRNAs have ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-104
更新日期:2014-05-06 00:00:00
abstract:BACKGROUND:miR-139-5p was identified to be significantly down-regulated in colon tumor tissues by miRNA array. We aimed to clarify its biological function, molecular mechanisms and direct target gene in colorectal cancer (CRC). METHODS:The biological function of miR-139-5p was examined by cell growth, cell cycle and a...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-124
更新日期:2014-05-26 00:00:00
abstract:BACKGROUND:Previous studies have established that levels of the Inhibitor of Growth 1(ING1) tumor suppressor are reduced in a significant proportion of different cancer types. Here we analyzed levels of ING1 in breast cancer patients to determine its prognostic significance as a biomarker for breast cancer prognosis. ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-015-0434-x
更新日期:2015-08-27 00:00:00
abstract:BACKGROUND:The epithelial-to-mesenchymal transition (EMT) process results in a loss of cell-cell adhesion, increased cell mobility, and is crucial for enabling the metastasis of cancer cells. Recently, the enzyme SIRT1 has been implicated in a variety of physiological processes; however, its role in regulating oral can...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-254
更新日期:2014-11-26 00:00:00
abstract:BACKGROUND:PTEN is the second most mutated tumor suppressor gene other than p53. It suppresses tumorigenesis by dephosphorylating phosphatidylinositol (3,4,5)-triphosphate (PIP3) to phosphatidylinositol (4,5)-biphosphate (PIP2), thereby directly inhibiting phosphatidylinositol 3 kinase (PI3K)-mediated tumorigenic activ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-98
更新日期:2009-11-10 00:00:00
abstract:BACKGROUND:Recent advances have revealed a significant contribution of chemokines and their receptors in tumor growth, survival after chemotherapy, and organ-specific metastasis. The CXC chemokine receptor-7 (CXCR7) is the latest chemokine receptor implicated in cancer. Although over expressed in breast cancer cell lin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-13-198
更新日期:2014-08-28 00:00:00
abstract:BACKGROUND:Recent studies indicate that circular RNA (circRNA) plays a pivotal role in cancer progression. Here, we sought to investigate its role in breast cancer. METHODS:CircANKS1B (a circRNA originated from exons 5 to 8 of the ANKS1B gene, hsa_circ_0007294) was identified by RNA-sequencing and validated by qRT-PCR...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-018-0914-x
更新日期:2018-11-19 00:00:00
abstract:BACKGROUND:New blood vessel formation, or angiogenic switch, is an essential event in the development of solid tumors and their metastatic growth. Tumor blood vessel formation and remodeling is a complex and multi-step processes. The differentiation and recruitment of mural cells including vascular smooth muscle cells ...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-209
更新日期:2010-08-05 00:00:00
abstract:BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies. Neovascularization during tumorigenesis supplies oxygen and nutrients to proliferative tumor cells, and serves as a conduit for migration. Targeting oncogenes involved in angiogenesis is needed to treat organ-confined and local...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-018-0930-x
更新日期:2019-01-04 00:00:00
abstract:BACKGROUND:We have previously reported that expression of the Wnt antagonist genes SFRP1 and SFRP5 is frequently silenced by promoter hypermethylation in breast cancer. SFRP2 is a further Wnt inhibitor whose expression was recently found being downregulated in various malignancies. Here we investigated whether SFRP2 is...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-7-83
更新日期:2008-11-06 00:00:00
abstract:BACKGROUND:Deregulated circular RNAs (circRNAs) are associated with the development of cancer and therapy resistance. However, functional research of circRNAs mostly focus on potential miRNA or protein binding and more potential regulation of circRNA on host gene DNA in cancers are yet to be inspected. METHOD:We perfo...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-020-01246-x
更新日期:2020-08-24 00:00:00
abstract:BACKGROUND:CD44 is considered as 'a' metastasis associated gene, despite the fact that it is an umbrella term for a group of molecules produced from a single gene by alternative splicing. However, little consideration is given to the above in the literature of colorectal carcinomas as well as other tumour types, leadin...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-11-83
更新日期:2012-11-14 00:00:00
abstract:BACKGROUND:Sorafenib, a kinase inhibitor active against various solid tumours, induces oxidative stress and ferroptosis, a new form of oxidative necrosis, in some cancer cells. Clinically-applicable biomarkers that reflect the impact of sorafenib on the redox metabolism of cancer cells are lacking. METHODS:We used gen...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/s12943-016-0526-2
更新日期:2016-05-16 00:00:00
abstract:BACKGROUND:Akt/PKB is a serine/threonine kinase that has attracted much attention because of its central role in regulating cell proliferation, survival, motility and angiogenesis. Activation of Akt in breast cancer portends aggressive tumour behaviour, resistance to hormone-, chemo-, and radiotherapy-induced apoptosis...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-8-58
更新日期:2009-08-03 00:00:00
abstract:BACKGROUND:Oncolytic virotherapy is an attractive drug platform of cancer gene therapy, but efficacy and specificity are important prerequisites for success of such strategies. Previous studies determined that Apoptin is a p53 independent, bcl-2 insensitive apoptotic protein with the ability to specifically induce apop...
journal_title:Molecular cancer
pub_type: 杂志文章
doi:10.1186/1476-4598-9-10
更新日期:2010-01-20 00:00:00
abstract::After the publication of this work [1], an error was found in Fig. 1b. As described in the Results section that circ0084003 has 13 exons, it should be formed by 5-17 (13exons) exons, authors have described "formed by 5-19 exons". The authors extend their apology for the mistake caused by their action. With that mistak...
journal_title:Molecular cancer
pub_type: 已发布勘误
doi:10.1186/s12943-020-1131-y
更新日期:2020-02-01 00:00:00