Detection of malaria parasites by nested PCR in south-eastern, Iran: evidence of highly mixed infections in Chahbahar district.


BACKGROUND:Rapid diagnosis and correct treatment of cases are the main objectives of control programs in malaria-endemic areas. METHODS AND RESULTS:To evaluate these criteria and in a comparative study, blood specimens were collected from 120 volunteers seeking care at the Malaria Health Center in Chahbahar district. One hundred and seven out of 120 Giemsa-stained slides were positive for malaria parasites by microscopy. Eighty-four (70%) and 20 (16.7%) were identified as having only Plasmodium vivax and Plasmodium falciparum infections, respectively, while only 3 (2.5%) were interpreted as having mixed P. vivax-P. falciparum infections. The target DNA sequence of the 18S small sub-unit ribosomal RNA (ssrRNA) gene was amplified by Polymerase Chain Reaction (PCR) and used for the diagnosis of malaria in south-eastern Iran. One hundred twenty blood samples were submitted and the results were compared to those of routine microscopy. The sensitivity of PCR for detection of P. vivax and P. falciparum malaria was higher than that of microscopy: nested PCR detected 31 more mixed infections than microscopy and parasite positive reactions in 9 out of the 13 microscopically negative samples. The results also confirmed the presence of P. vivax and P. falciparum. CONCLUSIONS:These results suggest that, in places where transmission of both P. vivax and P. falciparum occurs, nested PCR detection of malaria parasites can be a very useful complement to microscopical diagnosis.


Malar J


Malaria journal


Zakeri S,Najafabadi ST,Zare A,Djadid ND





Has Abstract


2002-02-08 00:00:00








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    pub_type: 杂志文章


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    更新日期:2018-12-14 00:00:00

  • An intricate case of multidrug resistant Plasmodium falciparum isolate imported from Cambodia.

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    更新日期:2016-11-09 00:00:00

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    abstract:BACKGROUND:Nicotinamide adenine dinucleotide (NAD+) is an essential molecule in the energy metabolism of living beings, and it has various cellular functions. The main enzyme in the biosynthesis of this nucleotide is nicotinamide/nicotinate mononucleotide adenylyltransferase (NMNAT, EC because it is the con...

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    abstract:BACKGROUND:There remains a need for techniques that improve the sensitive detection of viable Plasmodium falciparum as part of diagnosis and therapeutic monitoring in clinical studies and usual-care management of malaria infections. A non-invasive breath test based on P. falciparum-associated specific volatile organic ...

    journal_title:Malaria journal

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    abstract:BACKGROUND:The effectiveness of vector control on malaria transmission by long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) depends on the vectors entering houses to blood feed and rest when people are inside houses. In the Solomon Islands, significant reductions in malaria have been achieved in...

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    journal_title:Malaria journal

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    authors: Naeem MA,Ahmed S,Khan SA

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    authors: Basseri HR,Doosti S,Akbarzadeh K,Nateghpour M,Whitten MM,Ladoni H

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    abstract::The general paradigm that emerges from the analysis of the transcriptome of the malaria parasite Plasmodium falciparum is that the expression clusters of genes that code for enzymes engaged in the same cellular function is coordinated. Here the consistency of this perception is examined by analysing specific pathways ...

    journal_title:Malaria journal

    pub_type: 杂志文章


    authors: Bozdech Z,Ginsburg H

    更新日期:2005-03-18 00:00:00

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    abstract:BACKGROUND:The immunological mechanisms responsible for protection against malaria infection vary among Plasmodium species, host species and the developmental stage of parasite, and are poorly understood. A challenge with live parasites is the most relevant approach to testing the efficacy of experimental malaria vacci...

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    pub_type: 杂志文章


    authors: Leitner WW,Bergmann-Leitner ES,Angov E

    更新日期:2010-05-27 00:00:00

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    abstract:BACKGROUND:Plasmodium ovale, considered the rarest of the malaria parasites of humans, consists of two morphologically identical but genetically distinct sympatric species, Plasmodium ovale curtisi and Plasmodium ovale wallikeri. These parasites resemble morphologically to Plasmodium vivax with which they also share a ...

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    更新日期:2015-11-14 00:00:00

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    abstract:BACKGROUND:Programmes of pre-elimination of malaria have been implemented in Senegal since 2010, and the burden of malaria has decreased substantially. These changes in the epidemiology should be monitored with effective tools that allow changes in patterns of transmission to be estimated. In Dielmo and Ndiop, two vill...

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  • Comparison of the safety and efficacy of fixed-dose combination of arterolane maleate and piperaquine phosphate with chloroquine in acute, uncomplicated Plasmodium vivax malaria: a phase III, multicentric, open-label study.

    abstract:BACKGROUND:Chloroquine has been the treatment of choice for acute vivax malaria for more than 60 years. Malaria caused by Plasmodium vivax has recently shown resistance to chloroquine in some places. This study compared the efficacy and safety of fixed dose combination (FDC) of arterolane maleate and piperaquine phosph...

    journal_title:Malaria journal

    pub_type: 杂志文章,多中心研究


    authors: Valecha N,Savargaonkar D,Srivastava B,Rao BH,Tripathi SK,Gogtay N,Kochar SK,Kumar NB,Rajadhyaksha GC,Lakhani JD,Solanki BB,Jalali RK,Arora S,Roy A,Saha N,Iyer SS,Sharma P,Anvikar AR

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    journal_title:Malaria journal

    pub_type: 杂志文章,meta分析,评审


    authors: Eisenhut M,Omari A,MacLehose HG

    更新日期:2005-05-18 00:00:00

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    journal_title:Malaria journal

    pub_type: 杂志文章


    authors: Gebru T,Ajua A,Theisen M,Esen M,Ngoa UA,Issifou S,Adegnika AA,Kremsner PG,Mordmüller B,Held J

    更新日期:2017-04-26 00:00:00

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    journal_title:Malaria journal

    pub_type: 杂志文章


    authors: Odongo CO,Bisaso KR,Kitutu F,Obua C,Byamugisha J

    更新日期:2015-05-03 00:00:00

  • Central nervous system exposure of next generation quinoline methanols is reduced relative to mefloquine after intravenous dosing in mice.

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    journal_title:Malaria journal

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    authors: Dow GS,Milner E,Bathurst I,Bhonsle J,Caridha D,Gardner S,Gerena L,Kozar M,Lanteri C,Mannila A,McCalmont W,Moon J,Read KD,Norval S,Roncal N,Shackleford DM,Sousa J,Steuten J,White KL,Zeng Q,Charman SA

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    journal_title:Malaria journal

    pub_type: 杂志文章,meta分析


    authors: Adam I,Ibrahim Y,Gasim GI

    更新日期:2018-03-13 00:00:00

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    journal_title:Malaria journal

    pub_type: 杂志文章


    authors: Kayentao K,Florey LS,Mihigo J,Doumbia A,Diallo A,Koné D,Doumbo O,Eckert E

    更新日期:2018-11-14 00:00:00