Abstract:
BACKGROUND:The mechanism of aggressive character of ovarian cancer and unsuccessful treatment of women with this deadly disease has been recently explained by the theory of cancer stem cells (CSCs). It has been reported that ovarian carcinogenesis and progression of disease is associated with epithelial-mesenchymal transition (EMT). EMT, a physiological cell process during embryonic development and later in life during regeneration, could, when induced in pathological condition, generate CSCs-like cells. Until now EMT in the ovarian tissue has been mainly studied in cell cultures in vitro. The aim of this study was to focus on in situ morphological changes in the ovarian surface epithelium of tumor tissue in women with epithelial ovarian cancer after we applied the antibodies for markers of EMT vimentin and pluripotency-related markers NANOG, SOX2 and SSEA-4. METHODS:We analyzed ovarian tissue sections of 20 women with high grade serous ovarian carcinoma. After eosin and hematoxylin staining, used in standard practice, immunohistochemistry was performed for vimentin and markers of pluripotency: NANOG, SSEA-4 and SOX2. We focused on the ovarian surface epithelium in order to observe morphological changes in tumor tissue. RESULTS:Among epithelial cells of the ovarian surface epithelium in women with serous ovarian carcinoma we observed a population of small NANOG-positive cells with diameters of up to 5 μm and nuclei, which filled almost the entire cell volumes. These small NANOG-positive cells were in some cases concentrated in the regions with morphologically changed epithelial cells. In these regions, a population of bigger round cells with diameters of 10-15 μm with large nuclei, and positively stained for vimentin, NANOG and other markers of pluripotnecy, were released from the surface epithelium. These cells are proposed as CSCs, and possibly originate from small stem cells among epithelial cells. They formed typical cell clusters, invaded the tissue by changing their round shape into a mesenchymal-like phenotype, and contributed to the manifestation of ovarian cancer. CONCLUSIONS:Our findings show morphological changes in the ovarian surface epithelium in tumor slides of high grade serous ovarian carcinoma and provide a new population of putative CSCs.
journal_name
J Ovarian Resjournal_title
Journal of ovarian researchauthors
Kenda Suster N,Smrkolj S,Virant-Klun Idoi
10.1186/s13048-017-0306-7subject
Has Abstractpub_date
2017-02-23 00:00:00pages
11issue
1issn
1757-2215pii
10.1186/s13048-017-0306-7journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Whether there is a mechanistic link between FOLR1 and response to cisplatin has not been extensively examined. In this study, we determine the expression of FOLR1 in ovarian cancer and examine if FOLR1 levels influence response to cisplatin. RESULTS:(1) FOLR1 protein expression was lowest in normal ovarian ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-018-0387-y
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND:Ovarian hyperstimulation syndrome (OHSS) is a rare, yet severe, iatrogenic complication of ovulation induction therapy during assisted reproductive procedures. Our group previously detected atypical cells in the ascitic fluid of OHSS patients, although no malignancy developed during follow up. Here, the aim ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00624-9
更新日期:2020-03-05 00:00:00
abstract:BACKGROUND:This study was designed to investigate leptin levels in the fluid in ovarian endometriomas (OEs) and to compare the expression of leptin and its receptors (OBR) in ovarian tissue affected by endometrioma in infertile women to its expression in the normal ovarian tissue of fertile controls without endometrios...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-2
更新日期:2014-01-08 00:00:00
abstract:BACKGROUND:In this study we aimed to identify a prognostic signature in BRCA1/2 mutations to predict disease progression and the efficiency of chemotherapy ovarian cancer (OV), the second most common cause of death from gynecologic cancer in women worldwide. METHODS:Univariate Cox proportional-hazards and multivariate...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00712-w
更新日期:2020-09-19 00:00:00
abstract:OBJECTIVE:To explore the risk factors for the recurrence of endometrioma and the risk factors for the recurrence of endometriosis-related pain after long-term follow-up. METHODS:This study retrospectively analyzed 358 women with endometriomas who had a minimum of 5-years follow up after laparoscopic endometrioma excis...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-019-0552-y
更新日期:2019-08-30 00:00:00
abstract:BACKGROUND:To demonstrate the use of surface-enhanced Raman spectroscopy (SERS) to determine sialic acid (SA) levels in saliva using silver nanoparticles as substrates, in adnexal mass patients scheduled for surgical intervention to remove invasive masses, with the aim to compare SA levels in benign tumor vs ovarian ca...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-018-0433-9
更新日期:2018-07-24 00:00:00
abstract::Mixed germ cell tumours of the ovary are malignant neoplasms of the ovary comprising of two or more types of germ cell components. Most of the malignant mixed germ cell tumours consists of dysgerminoma accompanied by endodermal sinus tumours, immature teratoma or choriocarcinoma. There are only few case reports of mix...
journal_title:Journal of ovarian research
pub_type: 杂志文章,评审
doi:10.1186/s13048-014-0091-5
更新日期:2014-11-04 00:00:00
abstract::Ovarian hyperstimulation syndrome (OHSS) is a serious complication of controlled ovarian hyperstimulation (COH). The syndrome almost always presents either after hCG administration in susceptible patients or during early pregnancy. Despite many years of clinical experience, there are no precise methods to completely p...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-6-77
更新日期:2013-11-05 00:00:00
abstract:BACKGROUND:Hypertensive disorders of pregnancy are the most common causes of adverse maternal & perinatal outcomes. Such investigations in resource limited settings would help to have great design strategies in preventing maternal and perinatal morbidity and mortality. AIM:To determine management outcome and factor as...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0135-5
更新日期:2015-03-15 00:00:00
abstract:BACKGROUND:Mucinous carcinomas from different organs are morphologically similar and might share similarities at the molecular and biochemical levels that may illuminate their pathogenesis and influence management. The factors involved in the pathogenesis of mucinous carcinomas remain unknown; which is likely one contr...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-015-0169-8
更新日期:2015-06-17 00:00:00
abstract:BACKGROUND:To investigate dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for assessing histopathological and molecular biological features in induced rat epithelial ovarian carcinomas (EOCs). METHODS:7,12-dimethylbenz[A]anthracene (DMBA) was applied to induce EOCs in situ in 46 SD rats. Conventional MR...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0362-z
更新日期:2017-09-26 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate the impact of metabolic parameters in the peritoneal cavity on the likelihood of achieving complete tumor debulking in patients with ovarian and peritoneal cancers. MATERIALS AND METHODS:Forty-nine patients with ovarian and peritoneal cancers were included, who underwent pre-operat...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00662-3
更新日期:2020-05-20 00:00:00
abstract:BACKGROUND:Low adiponectin levels in polycystic ovarian syndrome (PCOS) have been largely attributed to obesity which is common among these patients. In addition, evidence also suggests that low adiponectin in PCOS may be related to insulin resistance (IR) in these women. However, studies on the role of adiponectin in ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-18
更新日期:2014-02-07 00:00:00
abstract:UNLABELLED: BACKGROUND:To investigate the 3.0-Tesla (3 T) magnetic resonance imaging (MRI) characteristics of primary adnexal lesions for discriminating benign from malignant lesions. METHODS:One hundred thirty-nine patients with pathologically proven primary adnexal masses referred for 3 T MRI assessment preoperativ...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-5-33
更新日期:2012-11-14 00:00:00
abstract:BACKGROUND:It is estimated that more than 20% of ovarian cancer cases are associated with a genetic predisposition that is only partially explained by germline mutations in the BRCA1 and BRCA2 genes. Recently, several pieces of evidence showed that mutations in three genes involved in the homologous recombination DNA r...
journal_title:Journal of ovarian research
pub_type: 杂志文章,meta分析
doi:10.1186/s13048-020-00654-3
更新日期:2020-05-02 00:00:00
abstract::Following publication of the original article [1], the authors opted to correct the incorrect e-mail address of the corresponding author from 82-55-360-1850chungsu1982@gmail.com to chungsu1982@gmail.com , grant in the Acknowledgements section and Author details. Below are the updated version of the Acknowledgements an...
journal_title:Journal of ovarian research
pub_type: 已发布勘误
doi:10.1186/s13048-019-0540-2
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:The down-regulation of E-cadherin gene (CDH1) expression has been regarded as an important event in cancer invasion and metastasis. However, the association between CDH1 promoter methylation and ovarian cancer remains unclear. A meta-analysis was conducted to evaluate the potential role of CDH1 promoter meth...
journal_title:Journal of ovarian research
pub_type: 杂志文章,meta分析
doi:10.1186/s13048-016-0231-1
更新日期:2016-04-11 00:00:00
abstract:UNLABELLED: BACKGROUND:Women with polycystic ovary syndrome (PCOS) have higher risk for cardiovascular disease (CVD). Heart type fatty acid binding protein (HFABP) has been found to be predictive for myocardial ischemia.Wet ested whether HFABP is the predictor for CVD in PCOS patients, who have an increased risk of ca...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-5-45
更新日期:2012-12-18 00:00:00
abstract:BACKGROUND:The polycystic ovary syndrome (PCOS) affects approximately 6-10% of women of reproductive age and is characterized by chronic anovulation and hyperandrogenism. However, a comprehensive understanding of the mechanisms that dictate androgen overproduction is lacking, which may account for inconsistencies betwe...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-5-15
更新日期:2012-05-18 00:00:00
abstract::Currently, the world is in the seventh month of the COVID-19 pandemic. Globally, infections with novel SARS-CoV-2 virus are continuously rising with mounting numbers of deaths. International and local public health responses, almost in synchrony, imposed restrictions to minimize spread of the virus, overload of health...
journal_title:Journal of ovarian research
pub_type: 杂志文章,评审
doi:10.1186/s13048-020-00737-1
更新日期:2020-11-27 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-021-00770-8
更新日期:2021-01-23 00:00:00
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journal_title:Journal of ovarian research
pub_type: 杂志文章,已发布勘误
doi:10.1186/s13048-020-00628-5
更新日期:2020-03-13 00:00:00
abstract::We have earlier reported the presence of very small embryonic-like stem cells (VSELs) in adult mouse uterus along with slightly bigger progenitors termed endometrial stem cells (EnSCs) and their regulation by ovarian hormones thus demonstrating a crucial role played by them during proliferation, differentiation and re...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0324-5
更新日期:2017-04-24 00:00:00
abstract:BACKGROUND:Ovarian cancer (OC) was the primary malignant gynecological cancer and SNARE protein is closely related with tumor progression. Here, we identified SNAP23, a member of SNARE complex, as a potential oncogene in OC. METHODS:We determined the expression of SNAP23 in OC tissues and explored the clinical signifi...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0289-9
更新日期:2016-11-17 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Journal of ovarian research
pub_type: 已发布勘误
doi:10.1186/s13048-020-00733-5
更新日期:2020-11-12 00:00:00
abstract::As the human ovarian follicle enlarges in the course of a regular cycle or following controlled ovarian stimulation, the changes in its structure reveal the oocyte environment composed of cumulus oophorus cells and the follicular fluid (FF).In contrast to the dynamic nature of cells, the fluid compartment appears as a...
journal_title:Journal of ovarian research
pub_type: 杂志文章,评审
doi:10.1186/s13048-017-0342-3
更新日期:2017-07-12 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the concentrations of IgG antibodies against Hsp60 and Hsp65 in sera of patients with ovarian cancer at various stages of clinical progress and for different histopathological types of disease. METHODS:Serum samples from 149 patients with ovarian carcinoma and 80 health...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/1757-2215-7-30
更新日期:2014-03-11 00:00:00
abstract:BACKGROUND:Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural antioxidant polyphenol able to exert a wide range of biological effect on several tissues. Despite its important beneficial properties, it has a low water solubility, which limits its therapeutic applications in humans. Resveratrol also acts as a ph...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-017-0357-9
更新日期:2017-09-15 00:00:00
abstract:BACKGROUND:Wnt-inducible signaling pathway protein 2 (WISP2) is a wnt1-induced signaling pathway protein 2. Although studies indicate that WISP2 may promote the development of various tumors, its role in ovarian cancer remains unclear. The objective of the current study was to analyze the effects of WISP2 on the prolif...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-020-00687-8
更新日期:2020-07-25 00:00:00
abstract:BACKGROUND:Ovarian thecoma-fibroma groups (OTFG) are uncommon sex cord-stromal neoplasms. The objective of the study was to demonstrate clinical and sonographic features of OTFG and compare with surgical histopathology. METHODS:A total of 61 patients with surgically proven OTFG were enrolled in this retrospective stud...
journal_title:Journal of ovarian research
pub_type: 杂志文章
doi:10.1186/s13048-016-0291-2
更新日期:2016-11-22 00:00:00