当前位置： SCI文献检索 > BMC Pulmonary Medicine期刊下所有文献 > Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.

Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.

Abstract：

BACKGROUND:IL-4 and IL-13 play a critical yet poorly understood role in orchestrating the recruitment and activation of effector cells of the asthmatic response and driving the pathophysiology of allergic asthma. The house dust mite (HDM) sheep asthma model displays many features of the human condition and is an ideal model to further elucidate the involvement of these critical Th2 cytokines. We hypothesized that airway exposure to HDM allergen would induce or elevate the expression profile of IL-4 and IL-13 during the allergic airway response in this large animal model of asthma. METHODS:Bronchoalveolar lavage (BAL) samples were collected from saline- and house dust mite (HDM)- challenged lung lobes of sensitized sheep from 0 to 48 h post-challenge. BAL cytokines (IL-4, IL-13, IL-6, IL-10, TNF-α) were each measured by ELISA. IL-4 and IL-13 expression was assessed in BAL leukocytes by flow cytometry and in airway tissue sections by immunohistology. RESULTS:IL-4 and IL-13 were increased in BAL samples following airway allergen challenge. HDM challenge resulted in a significant increase in BAL IL-4 levels at 4 h compared to saline-challenged airways, while BAL IL-13 levels were elevated at all time-points after allergen challenge. IL-6 levels were maintained following HDM challenge but declined after saline challenge, while HDM administration resulted in an acute elevation in IL-10 at 4 h but no change in TNF-α levels over time. Lymphocytes were the main early source of IL-4, with IL-4 release by alveolar macrophages (AMs) prominent from 24 h post-allergen challenge. IL-13 producing AMs were increased at 4 and 24 h following HDM compared to saline challenge, and tissue staining provided evidence of IL-13 expression in airway epithelium as well as immune cells in airway tissue. CONCLUSION:In a sheep model of allergic asthma, airway inflammation is accompanied by the temporal release of key cytokines following allergen exposure that primarily reflects the Th2-driven nature of the immune response in asthma. The present study demonstrates for the first time the involvement of IL-4 and IL-13 in a relevant large animal model of allergic airways disease.

journal_name

BMC Pulm Med

journal_title

BMC pulmonary medicine

authors

Liravi B,Piedrafita D,Nguyen G,Bischof RJ

doi

10.1186/s12890-015-0097-9

subject

Has Abstract

pub_date

2015-09-11 00:00:00

pages

101

issn

1471-2466

pii

10.1186/s12890-015-0097-9

journal_volume

pub_type

杂志文章

在线工具

Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge.