Abstract:
BACKGROUND:Chagas disease is caused by Trypanosoma cruzi, which is transmitted by triatomine vectors. The northeastern region of Brazil is endemic for Chagas disease and has the largest diversity of triatomine species. T. cruzi development in its triatomine vector depends on diverse factors, including the composition of bacterial gut microbiota. METHODS:We characterized the triatomines captured in the municipality of Russas (Ceará) by sequencing the cytochrome c oxidase subunit I (COI) gene. The composition of the bacterial community in the gut of peridomestic Triatoma brasiliensis and Triatoma pseudomaculata was investigated using culture independent methods based on the amplification of the 16S rRNA gene by polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), DNA fragment cloning, Sanger sequencing and 454 pyrosequencing. Additionally, we identified TcI and TcII types of T. cruzi by sequencing amplicons from the gut metagenomic DNA with primers for the mini-exon gene. RESULTS:Triatomines collected in the peridomestic ecotopes were diagnosed as T. pseudomaculata and T. brasiliensis by comparing their COI sequence with GenBank. The rate of infection by T. cruzi in adult triatomines reached 80% for T. pseudomaculata and 90% for T. brasiliensis. According to the DNA sequences from the DGGE bands, the triatomine gut microbiota was primarily composed of Proteobacteria and Actinobacteria. However, Firmicutes and Bacteroidetes were also detected, although in much lower proportions. Serratia was the main genus, as it was encountered in all samples analyzed by DGGE and 454 pyrosequencing. Members of Corynebacterinae, a suborder of the Actinomycetales, formed the next most important group. The cloning and sequencing of full-length 16S rRNA genes confirmed the presence of Serratia marcescens, Dietzia sp., Gordonia terrae, Corynebacterium stationis and Corynebacterium glutamicum. CONCLUSIONS:The study of the bacterial microbiota in the triatomine gut has gained increased attention because of the possible role it may play in the epidemiology of Chagas disease by competing with T. cruzi. Culture independent methods have shown that the bacterial composition of the microbiota in the guts of peridomestic triatomines is made up by only few bacterial species.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Gumiel M,da Mota FF,Rizzo Vde S,Sarquis O,de Castro DP,Lima MM,Garcia Ede S,Carels N,Azambuja Pdoi
10.1186/s13071-015-0836-zsubject
Has Abstractpub_date
2015-04-24 00:00:00pages
245issn
1756-3305pii
10.1186/s13071-015-0836-zjournal_volume
8pub_type
杂志文章abstract:BACKGROUND:Coccidiosis due to Eimeria spp. infections in lambs causes increased mortality and substantial production losses, and anticoccidials are important for control of the infection. Anticoccidial resistance has been reported in poultry and swine, and we recently described reduced toltrazuril efficacy in ovine Eim...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2976-4
更新日期:2018-07-05 00:00:00
abstract:BACKGROUND:The protozoan Toxoplasma gondii is the causative agent of toxoplasmosis, with complications varying from mental disease to death. While human infection can occur via ingestion of tissue cysts from infected meat, most human infection comes from oocysts. Cats are the only definitive host, and thus shedding of ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-266
更新日期:2013-09-17 00:00:00
abstract:BACKGROUND:The sibling species of the malaria mosquito, Anopheles gambiae (sensu stricto) and Anopheles coluzzii co-exist in many parts of West Africa and are thought to have recently diverged through a process of ecological speciation with gene flow. Divergent larval ecological adaptations, resulting in Genotype-by-En...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04483-7
更新日期:2021-01-06 00:00:00
abstract::Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their envir...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/s13071-018-3222-9
更新日期:2018-12-24 00:00:00
abstract:BACKGROUND:Sandfly-borne phleboviruses are present in North Africa where they can infect humans in regions where Leishmania infantum, the causative agent of zoonotic visceral leishmaniasis in the Western Mediterranean basin is present affecting both humans and dogs. We investigated the capacity of dogs to be used as se...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0476-8
更新日期:2014-10-12 00:00:00
abstract:BACKGROUND:Understanding local Anopheles species compositions and bionomic traits are vital for an effective malaria vector intervention strategy. Though eight malaria vectors, including species complexes, have been documented across the island of Sulawesi, Indonesia, a comprehensive survey linking morphological and mo...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04252-6
更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:Both falciparum and vivax malaria were historically prevalent in China with high incidence. With the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000. Despite this, the re-emergence or outbreak of malaria was unavoidable...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-136
更新日期:2012-07-09 00:00:00
abstract:BACKGROUND:Intestinal parasite infections (IPIs) are among the most significant causes of illness and disease of socially and economically disadvantaged populations in developing countries, including rural areas of the People's Republic of China. With the spread of the human immunodeficiency virus (HIV) among rural Chi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-36
更新日期:2012-02-13 00:00:00
abstract:BACKGROUND:Schistosoma mansoni cercariae penetrate the skin by releasing excretory/secretory (E/S) products known as 0-3hRP, which are associated with immune modulation through Toll like receptor (TLR) signalling. Furthermore, these secretions contain Sm16, which when given to cells as a recombinant protein inhibits hu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0608-1
更新日期:2015-01-06 00:00:00
abstract:BACKGROUND:Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the serop...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1753-5
更新日期:2016-08-24 00:00:00
abstract:BACKGROUND:Taenia solium is an important zoonotic parasite that infects humans as definitive host (taeniasis) and pigs as intermediate host (cysticercosis). Serological diagnosis of porcine cysticercosis is limited to antigen detection using ELISA, which is known to cross-react with other Taenia species, and antibody d...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-3279-5
更新日期:2019-01-24 00:00:00
abstract:BACKGROUND:Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii i...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0558-7
更新日期:2014-12-03 00:00:00
abstract::In the more than 100 years since its discovery, our knowledge of Toxoplasma biology has improved enormously. The evolution of molecular biology, immunology and genomics has had profound influences on our understanding of this ubiquitous bug. However, it could be argued that in science today the adage "seeing is believ...
journal_title:Parasites & vectors
pub_type: 历史文章,杂志文章,评审
doi:10.1186/1756-3305-6-334
更新日期:2013-11-22 00:00:00
abstract:BACKGROUND:Intestinal schistosomiasis due to Schistosoma mansoni was mapped in Cameroon in the 1990s and preventive chemotherapy launched since 2005. A situation analysis conducted in 2011 revealed an increase in schistosomiasis transmission, especially in the equatorial part of the country, despite the fact that Biomp...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2763-2
更新日期:2018-03-13 00:00:00
abstract:BACKGROUND:Dengue has become the subject of public health programs worldwide. The lack of a vaccine and the high environmental risk of synthetic insecticides, arouse the interest in natural products against this vector. This study aimed to determine the chemical composition of the essential oil of ripe fruits and seeds...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0746-0
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:Plasmodium falciparum delayed clearance with the use of artemisinin-based combination therapy (ACTs) has been reported in some African countries. Single nucleotide polymorphisms (SNPs) in two genes, P. falciparum adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1), have ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2762-3
更新日期:2018-03-12 00:00:00
abstract:BACKGROUND:Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, causing quite pronounced...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3429-4
更新日期:2019-04-25 00:00:00
abstract:BACKGROUND:The two most abundant sand fly species on the Honduran Pacific coast are Lutzomyia (Lutzomyia) longipalpis and Pintomyia (Pifanomyia) evansi. Both species are known vectors of Leishmania (Leishmania) infantum, the etiological agent of visceral leishmaniasis (VL) in the Americas. Although VL and non-ulcerativ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04462-y
更新日期:2020-11-23 00:00:00
abstract:BACKGROUND:Phlebotomine sand flies are dipterans of the family Psychodidae. They are very important to veterinary medicine because some species are vectors of infective forms of Leishmania spp., the etiological agents of leishmaniasis. The Parque Estadual do Rio Doce is located in an area with constant reports of cases...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1227-1
更新日期:2015-12-02 00:00:00
abstract:BACKGROUND:Northeastern Italy is a hotspot for several tick-borne pathogens, transmitted to animals and humans mainly by Ixodes ricinus. Here we compare the results of molecular monitoring of ticks and zoonotic TBPs over a six-year period, with the monitoring of red foxes (Vulpes vulpes) in an endemic area. RESULTS:In...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2726-7
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:Recent reports have demonstrated the presence of Crimean-Congo hemorrhagic fever virus (CCHFV) genomic material in Hyalomma aegyptium ticks feeding primarily on tortoises belonging to the genus Testudo. This raises the question if these ticks and their hosts play a role in the natural transmission dynamics o...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04074-6
更新日期:2020-04-19 00:00:00
abstract:BACKGROUND:African trypanosomes are extracellular protozoan parasites that are transmitted between mammalian hosts by the bite of an infected tsetse fly. Human African Trypanosomiasis (HAT) or sleeping sickness is caused by Trypanosoma brucei rhodesiense or T. brucei gambiense, while African Animal Trypanosomiasis (AAT...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-217
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:Hard ticks are hematophagous ectoparasites characterized by their long-term feeding. The saliva that they secrete during their blood meal is their crucial weapon against host-defense systems including hemostasis, inflammation and immunity. The anti-hemostatic, anti-inflammatory and immune-modulatory activiti...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2874-9
更新日期:2018-05-24 00:00:00
abstract:BACKGROUND:Rhipicephalus microplus is a hard tick that has a major impact on cattle health in tropical and subtropical regions because it feeds on cattle and is implicated in the transmission of pathogens that cause diseases such as bovine anaplasmosis and babesiosis. Presently, acaricides are used to control tick infe...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2081-0
更新日期:2017-03-23 00:00:00
abstract:BACKGROUND:The Culex pipiens complex (Diptera: Culicidae) includes the most widespread mosquito species in the world. Members of this complex are the primary enzootic and epidemic vectors of the West Nile virus (genus Flavivirus) in several countries. The two recognized forms of Cx. pipiens (Linnaeus, 1758) - pipiens a...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2265-7
更新日期:2017-07-10 00:00:00
abstract:BACKGROUND:Aedes albopictus is widely distributed across tropical and sub-tropical regions and is associated with the transmission of several arboviruses. Although this species is increasingly relevant to public health due its ability to successfully colonize both urban and rural habitats, favoring the dispersion of vi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-253
更新日期:2014-05-30 00:00:00
abstract:BACKGROUND:Thelazia callipaeda is transmitted by Phortica variegata, a drosophilid that feeds on lachrymal secretions of mammals. Scientific information on human thelaziosis is still relatively limited, mainly for physicians and ophthalmologists. Indeed, the literature is full of misleading information on the transmiss...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0881-7
更新日期:2015-05-14 00:00:00
abstract:BACKGROUND:Air-borne introduction of exotic mosquitoes to Schiphol airport in the Netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. Beginning in 2013, surveillance efforts at Schiphol had focused on promptly detecting accidental introductions at t...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2555-0
更新日期:2017-12-08 00:00:00
abstract:BACKGROUND:Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. FINDINGS:Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were se...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-178
更新日期:2013-06-17 00:00:00
abstract:BACKGROUND:Angiostrongylus cantonensis is an important causative agent of eosinophilic meningitis and eosinophilic meningoencephalitis in humans. Previous studies have shown that the Sonic hedgehog (Shh) signaling pathway may reduce cell apoptosis by inhibiting oxidative stress in A. cantonensis infection. In this stud...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2385-0
更新日期:2017-09-26 00:00:00