Abstract:
BACKGROUND:Hypervariable region 1 (HVR1) contained within envelope protein 2 (E2) gene is the most variable part of HCV genome and its translation product is a major target for the host immune response. Variability within HVR1 may facilitate evasion of the immune response and could affect treatment outcome. The aim of the study was to analyze the impact of HVR1 heterogeneity employing sensitive ultra-deep sequencing, on the outcome of PEG-IFN-α (pegylated interferon α) and ribavirin treatment. METHODS:HVR1 sequences were amplified from pretreatment serum samples of 25 patients infected with genotype 1b HCV (12 responders and 13 non-responders) and were subjected to pyrosequencing (GS Junior, 454/Roche). Reads were corrected for sequencing error using ShoRAH software, while population reconstruction was done using three different minimal variant frequency cut-offs of 1%, 2% and 5%. Statistical analysis was done using Mann-Whitney and Fisher's exact tests. RESULTS:Complexity, Shannon entropy, nucleotide diversity per site, genetic distance and the number of genetic substitutions were not significantly different between responders and non-responders, when analyzing viral populations at any of the three frequencies (≥1%, ≥2% and ≥5%). When clonal sample was used to determine pyrosequencing error, 4% of reads were found to be incorrect and the most abundant variant was present at a frequency of 1.48%. Use of ShoRAH reduced the sequencing error to 1%, with the most abundant erroneous variant present at frequency of 0.5%. CONCLUSIONS:While deep sequencing revealed complex genetic heterogeneity of HVR1 in chronic hepatitis C patients, there was no correlation between treatment outcome and any of the analyzed quasispecies parameters.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Cortés KC,Zagordi O,Perlejewski K,Laskus T,Maroszek K,Bukowska-Ośko I,Pawełczyk A,Płoski R,Berak H,Horban A,Radkowski Mdoi
10.1186/1471-2334-14-389subject
Has Abstractpub_date
2014-07-13 00:00:00pages
389issn
1471-2334pii
1471-2334-14-389journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Young people are disproportionately affected by sexually transmissible infections in Australia but face barriers to accessing sexual health services, including concerns over confidentiality and, for some, geographic remoteness. A possible innovation to increase access to services is the use of telemedicine. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-285
更新日期:2011-10-25 00:00:00
abstract:BACKGROUND:Why human tick-borne encephalitis (TBE) cases differ from year to year, in some years more 100%, has not been clarified, yet. The cause of the increasing or decreasing trends is also controversial. Austria is the only country in Europe where a 40-year TBE time series and an official vaccine coverage time ser...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05156-7
更新日期:2020-06-26 00:00:00
abstract:BACKGROUND:Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic dengue fever or dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1271-7
更新日期:2015-11-14 00:00:00
abstract:BACKGROUND:Previously thought to be rabies free, Bali experienced an outbreak of animal and human rabies cases in November 2008. We describe the epidemiological and clinical data of human rabies cases occurring in the first two years of the outbreak. METHODS:We analysed the patient records of all rabies cases from the...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-81
更新日期:2012-04-02 00:00:00
abstract:BACKGROUND:Dengue, a viral disease transmitted by Aedes mosquitoes, is an important public health concern throughout Thailand. Climate variables are potential predictors of dengue transmission. Associations between climate variables and dengue have usually been performed on large-scale first-level national administrati...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4379-3
更新日期:2019-08-23 00:00:00
abstract:BACKGROUND:Chronic infections and associated inflammatory markers are suggested risk factors for cardiovascular disease (CVD). The proinflammatory cytokine, interleukin (IL)-1β, is suggested to play a role in the regulation of local inflammatory responses in both CVD and periodontitis. The leukotoxin from the periodont...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-55
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:The tuberculin skin test (TST) has limitations for latent tuberculosis infection (LTBI) diagnosis in low-prevalence settings. Previously, all TST-positive individuals referred from the community to Baltimore City Health Department (BCHD) were offered LTBI treatment, after active TB was excluded. In 2010, BCH...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-360
更新日期:2012-12-19 00:00:00
abstract:BACKGROUND:To assess the knowledge, the attitudes, and the behaviour towards influenza A/H1N1 and the vaccination among health-care workers (HCWs). METHODS:A sample of HCWs was selected from a random sample of non-teaching public hospitals, located in the cities of Naples and Avellino (Italy), received a self-administ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-208
更新日期:2014-04-16 00:00:00
abstract:BACKGROUND:Chronic kidney disease, including end-stage renal disease, has been identified as a possible risk factor for primary septicemia and wound infection by Vibrio vulnificus. However, cases of severe septicemia, necrotizing fasciitis, and peritonitis caused by V. vulnificus in patients undergoing continuous ambul...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1163-x
更新日期:2015-10-14 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa is a Gram-negative bacteria responsible for infections in immunocompromised patients and is one of the most common causes of nosocomial infections particularly in intensive care and burn units. We aimed to investigate the population structure of P. aeruginosa strains isolated from pati...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/s12879-020-05404-w
更新日期:2020-09-22 00:00:00
abstract:BACKGROUND:Qinghai-Tibetan Plateau of China is known to be the plague endemic region where marmot (Marmota himalayana) is the primary host. Human plague cases are relatively low incidence but high mortality, which presents unique surveillance and public health challenges, because early detection through surveillance ma...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-382
更新日期:2014-07-10 00:00:00
abstract:BACKGROUND:This study presents an analysis of 1,491 serogroup 23 and 762 serogroup 15 isolates from invasive pneumococcal disease (IPD) in children and adults before and after the general recommendation for childhood pneumococcal conjugate vaccination in Germany in July 2006. Vaccination formulations used were PCV7 (fr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-0941-9
更新日期:2015-05-05 00:00:00
abstract:BACKGROUND:Before Pre-Exposure Prophylaxis (PrEP) was officially recommended and made available, a few surveys among gay and bisexual men, and persons living with HIV/AIDS (PLWHA), identified an informal use of antiretrovirals (ARVs) for PrEP among HIV-negative individuals. Before PrEP availability in Italy, we aimed t...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2819-5
更新日期:2017-11-28 00:00:00
abstract:BACKGROUND:Chlamydia trachomatis is a pathogen of worldwide importance, causing more than 100 million cases of sexually transmitted infections annually. Whole-genome sequencing is a powerful high resolution tool that can be used to generate accurate data on bacterial population structure, phylogeography and mutations a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-014-0591-3
更新日期:2014-11-12 00:00:00
abstract:BACKGROUND:Although Candida albicans is the most common cause of fungal blood stream infections (BSIs), infections due to Candida species other than C. albicans are rising. Candida parapsilosis complex has emerged as an important fungal pathogen and became one of the main causes of fungemia in specific geographical are...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1704-y
更新日期:2016-08-09 00:00:00
abstract:BACKGROUND:A remote arctic region of Canada predominantly populated by Inuit with the country's highest incidence of tuberculosis. METHODS:The study was undertaken to describe the latent tuberculosis infection (LTBI) cascade of care and identify factors associated with non-initiation and non-completion of LTBI treatme...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4557-3
更新日期:2019-10-24 00:00:00
abstract:BACKGROUND:The real burden of smear-positive (PTB+) and bacteriologically confirmed tuberculosis (BCTB) in Ethiopia is not known. Thus, the aim of this community-based study was to measure the prevalence and incidence of tuberculosis in the Hetosa District of Oromia Region, Ethiopia. METHODS:First, a population-based ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2321-0
更新日期:2017-03-16 00:00:00
abstract:BACKGROUND:Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) in the Indian subcontinent. However, it is also known to cause cutaneous leishmaniasis (CL) in Sri Lanka. Sri Lankan L. donovani differs from other L. donovani strains, both at the molecular and biochemical level. To investigate the ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2883-x
更新日期:2017-12-22 00:00:00
abstract:BACKGROUND:ProvLab Alberta provides all laboratory testing for Bordetella pertussis including sporadic cases and outbreak investigations through collaborations with provincial public health partners. We describe B. pertussis activity in Alberta from July 2004 to December 2012. METHODS:Laboratory testing for pertussis ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-48
更新日期:2014-01-30 00:00:00
abstract:BACKGROUND:While several studies have assessed the associations between biological factors and tuberculosis (TB) transmission, our understanding of the associations between TB transmission and social and economic factors remains incomplete. We aimed to explore associations between community TB transmission and socio-ec...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4828-z
更新日期:2020-02-10 00:00:00
abstract:BACKGROUND:Estimation of pre-immunisation prevalence of HPV and distribution of HPV types is fundamental to understanding the subsequent impact of HPV vaccination. We describe the type specific prevalence of HPV in females aged 20-21 in Scotland who attended or defaulted from cervical screening using three specimen typ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-519
更新日期:2013-11-05 00:00:00
abstract:BACKGROUND:Refugees in sub-Saharan Africa face both the risk of HIV infection and barriers to HIV testing. We conducted a pilot study to determine the feasibility and acceptability of home-based HIV testing in Nakivale Refugee Settlement in Uganda and to compare home-based and clinic-based testing participants in Nakiv...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3238-y
更新日期:2018-07-16 00:00:00
abstract:BACKGROUND:The risk of life-threatening complications, such as visceral disseminated varicella zoster virus (VZV) infection, is greater in immunosuppressed individuals, such as systemic lupus erythematosus (SLE) patients. CASE PRESENTATION:Here, a case is reported of a Caucasian woman diagnosed with lupus nephritis an...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05254-6
更新日期:2020-07-23 00:00:00
abstract:BACKGROUND:Although breast-feeding accounts for 15-20% of mother-to-child transmission (MTCT) of HIV, it is not prohibited in some developing countries because of the higher mortality associated with not breast-feeding. We assessed the potential impact, on HIV infection and infant mortality, of a recommendation for sho...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-66
更新日期:2008-05-16 00:00:00
abstract:BACKGROUND:Leprosy still remains an important public health problem for many parts of the world. An association of gangrene with leprosy is a rare one & can have a number of causative mechanisms. We present a case with Leprosy & gangrene with positive anti phopholipid antibody titers. CASE PRESENTATION:A 50-year-old n...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-5-74
更新日期:2005-09-21 00:00:00
abstract:BACKGROUND:Hepatocellular Carcinoma (HCC) is one of the commonest cancers in Central Africa, a region with the unusual peculiarity to be hyperendemic for infections with Hepatitis B, C and D viruses. However, data estimating the respective proportions of HCC cases attributable to these viruses are still limited in this...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1992-2
更新日期:2016-11-07 00:00:00
abstract:BACKGROUND:Community-acquired pneumonia (CAP) remains a major cause of morbidity and mortality throughout the world. Telithromycin (a new ketolide) has shown good in vitro activity against the key causative pathogens of CAP, including S pneumoniae resistant to penicillin and/or macrolides. METHODS:The efficacy and saf...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/1471-2334-5-43
更新日期:2005-05-31 00:00:00
abstract:BACKGROUND:The ocular manifestations of HIV may lead to visual impairment or blindness. In India, patients typically initiate antiretroviral treatment (ART) with low CD4 cell counts when the risk of ocular complications may be high. The objective of this study was to determine the prevalence and types of HIV-associated...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-9-158
更新日期:2009-09-23 00:00:00
abstract:BACKGROUND:Approximately 50% of cases of penile carcinoma (PeCa), a rare neoplasm worldwide, are associated with human papillomavirus (HPV). However, the detection of HPV-DNA is not sufficient to consider it the etiological factor in the development of this type of cancer. Currently, the overexpression of P16INK4A is u...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4696-6
更新日期:2019-12-19 00:00:00
abstract:BACKGROUND:The C - reactive protein (CRP) response is often measured in patients with active tuberculosis (TB) yet little is known about its relationship to clinical features in TB, or whether responses differ between ethnic groups or with different Mycobacterium tuberculosis (M.tb) strain types. We report the relation...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1612-1
更新日期:2016-06-10 00:00:00