Abstract：
:The present study investigated the presence of ryanodine receptors in the trematode Schistosoma mansoni. [3H]Ryanodine specific binding sites were found in the four subcellular fractions of S. mansoni; however, more binding sites were recovered in the heterogeneous fraction P1 and the microsomal fraction P4, as was thapsigargin-sensitive (Ca2+-Mg2+)ATPase activity, marking the sarco/endoplasmic reticulum calcium ATPase (SERCA) pumps. This binding had an equilibrium dissociation constant (Kd) in the nanomolar range, an apparent maximal number of receptors (Bmax) of about 80 fmol/mg of protein, and was modulated by ions (Ca2+, Mg2+) and some pharmacological tools such as caffeine. Ryanodine was able to accelerate the rate of 45Ca2+ release from actively loaded vesicles, and also to induce a transient contraction of the whole worm. We conclude that ryanodine-sensitive Ca2+ release channels are present in S. mansoni, with properties very similar to the ones present in higher animals.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Silva CL,Cunha VM,Mendonça-Silva DL,Noël Fdoi
10.1016/s0006-2952(98)00219-6subject
Has Abstractpub_date
1998-10-15 00:00:00pages
997-1003issue
8eissn
0006-2952issn
1873-2968pii
S0006-2952(98)00219-6journal_volume
56pub_type
杂志文章abstract：:The recognition sites for the 5-hydroxytryptamine (5-HT) uptake inhibitors imipramine and paroxetine may represent receptors for a presently unknown endogenous ligand, whose function would be to modulate 5-HT uptake. Attempts to isolate such a factor from rat brain tissue are described, following a published procedure...
journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1987-03-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(97)00148-2
更新日期：1997-07-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/s0006-2952(00)00563-3
更新日期：2001-02-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(95)00236-s
更新日期：1995-09-28 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2000-02-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.bcp.2010.06.012
更新日期：2010-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-07-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1998-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：1993-10-19 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(88)90317-6
更新日期：1988-08-15 00:00:00
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pub_type： 杂志文章
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更新日期：1989-03-01 00:00:00
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pub_type： 杂志文章
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1993-07-20 00:00:00
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更新日期：1982-04-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(87)90377-7
更新日期：1987-01-01 00:00:00
abstract：:This article has been retracted consistent with Elsevier Policy on Article Withdrawal. Please see . The Publisher apologises for any inconvenience this may cause. ...
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pub_type： 杂志文章
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更新日期：2006-04-18 00:00:00
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pub_type： 杂志文章
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更新日期：2002-05-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1995-10-26 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(85)90277-1
更新日期：1985-12-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(95)02182-5
更新日期：1996-03-22 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(84)90710-x
更新日期：1984-08-01 00:00:00
