Abstract：
:Forskolin, a diterpene compound isolated from the roots of Coleus forskohlii, activates adenylate cyclase in membranes from a variety of mammalian tissues. We found that forskolin (10(-7) to 3 X 10(-5) M) caused a concentration-related inhibition of IgE-mediated release of histamine and peptide leukotriene C4 (LTC4) from human basophils and lung mast cells. There was a significant linear correlation between the per cent inhibition of histamine and LTC4 release from both cell types. However, in both systems forskolin exerted a significantly greater inhibitory effect on LTC4 release than on histamine release. The concentration-response inhibition curve was paralleled by a forskolin-induced rise in cAMP levels in human leukocyte and mast cell preparations. The relationship between the effect of forskolin and the cAMP concentration was supported by the finding that forskolin inhibited the "first stage" of antigen-induced histamine release, but not the release caused by the Ca2+ ionophore, A23187. Propranolol, a competitive beta-receptor antagonist, did not block the inhibition of mediator release or the cAMP accumulation caused by forskolin. These data suggest that forskolin modulates the release of mediators of immediate hypersensitivity reactions via the activation of adenylate cyclase in human basophils and mast cells.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Marone G,Columbo M,Triggiani M,Cirillo R,Genovese A,Formisano Sdoi
10.1016/0006-2952(87)90377-7subject
Has Abstractpub_date
1987-01-01 00:00:00pages
13-20issue
1eissn
0006-2952issn
1873-2968pii
0006-2952(87)90377-7journal_volume
36pub_type
杂志文章abstract：:Taxol-based chemotherapy is widely used as the first-line treatment for non-small cell lung cancer (NSCLC), however, the subsequent development of taxol-resistance is a major concern and challenge, resulting in tumor relapse and poor prognosis. Given the complex nature of taxol-resistance, we further delved into its m...
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journal_title：Biochemical pharmacology
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更新日期：2001-03-15 00:00:00
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pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-01-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/j.bcp.2006.08.014
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1988-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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journal_title：Biochemical pharmacology
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1993-02-24 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(86)90110-3
更新日期：1986-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：2010-10-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1984-12-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章,评审
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更新日期：2012-09-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(96)00106-2
更新日期：1996-07-12 00:00:00
