Abstract:
:The production of interleukin-1beta (IL-1beta), a powerful mediator of inflammation, is tightly regulated at several levels. However, in some pathologic conditions, a pharmacologic treatment is required to control the toxicity of excessive extracellular IL-1beta. Because of the heavy side effects of most therapies used in IL-1beta-mediated pathologies, a goal of pharmacologic research is the development of selective anti-IL-1beta drugs. We show here that the sulfonylurea glyburide, currently used in the oral therapy of noninsulin dependent diabetes, is an inhibitor of IL-1beta secretion from human monocytes and mouse macrophages. Glyburide reduces dramatically the recovery of extracellular 17-kD IL-1beta in the absence of toxic effects on the cells and without affecting the synthesis or processing of the IL-1beta precursor. IL-1beta belongs to the family of leaderless secretory proteins released from the cell by a nonclassical secretory route. In bacteria and yeast Atp binding cassette (ABC) transporters are involved in the secretion of leaderless secretory proteins. Interestingly, glyburide blocks the anion exchanger function of ABC1, a mammalian member of the family of ABC transporters. We thus investigated the involvement of ABC1 in IL-1beta secretion, through the analysis of the effects of drugs known to inhibit IL-1beta secretion, on the activity of ABC1 and in turn the ability of known inhibitors of ABC1 of blocking IL-1beta secretion. Our data show that IL-1beta secretion and the function of ABC1 as an anion exchanger are sensitive to the same drugs, therefore suggesting an involvement of the ABC1 transporter in the secretion of leaderless proteins in mammals.
journal_name
Bloodjournal_title
Bloodauthors
Hamon Y,Luciani MF,Becq F,Verrier B,Rubartelli A,Chimini Gsubject
Has Abstractpub_date
1997-10-15 00:00:00pages
2911-5issue
8eissn
0006-4971issn
1528-0020journal_volume
90pub_type
杂志文章相关文献
BLOOD文献大全abstract::LMP-1, an Epstein-Barr viral (EBV) latency protein, is considered a viral oncogene because of its ability to transform rodent fibroblasts in vivo and render them tumorigenic in nude mice. In human B cells, EBV LMP-1 induces DNA synthesis and abrogates apoptosis. LMP-1 is expressed in EBV-transformed lymphoblastoid cel...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Graft-versus-host disease (GVHD) remains one of the major complications after allogeneic bone marrow transplantation (allo-BMT). Sirtuin-1 (Sirt-1) plays a crucial role in various biological processes including cellular senescence, metabolism, and inflammatory responses. Sirt-1 deacetylation regulates different transc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-07-863233
更新日期:2019-01-17 00:00:00
abstract::Intravenous gammaglobulin (IVIgG) was used to treat autoimmune neutropenia of infancy in two males with repeated infections. The neutrophil count increased significantly in both patients with the initial IVIgG therapy; 1 patient went into remission. The neutrophil count in the other remained above baseline for 3 wk, a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Autologous T cells engineered to express a CD19-specific chimeric antigen receptor (CAR) have produced impressive minimal residual disease-negative (MRD-negative) complete remission (CR) rates in patients with relapsed/refractory B-cell acute lymphoblastic leukemia (B-ALL). However, the factors associated with durable...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-11-883710
更新日期:2019-04-11 00:00:00
abstract::Immunosenescence is the functional deterioration of the immune system during natural aging. Despite increased susceptibility to bacterial infections in older adults, age-associated changes to neutrophil responses are only partially understood, and neutrophil migration has not been characterized in detail. Here we desc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-08-519520
更新日期:2014-01-09 00:00:00
abstract::Foxp3(+) regulatory T cells (Tregs) maintain self-tolerance and adoptive therapy, and using Foxp3(+) Tregs has been proposed as treatment for autoimmune diseases. The clinical use of Tregs will require large numbers of cells and methods for in vitro expansion of Tregs are being developed. Foxp3(+) Tregs can be divided...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-09-377895
更新日期:2012-03-22 00:00:00
abstract::The non-Hodgkin's lymphoma (NHL) subset commonly referred to as large cell lymphoma (LCL) has historically been characterized by it's marked cytological, immunological, and clinical heterogeneity. One potential defining feature of these lymphomas, the t(2;5)(p23;q35), occurs in 25% to 30% of anaplastic LCLs and is als...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::CD177 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed by a variable proportion of human neutrophils that mediates surface expression of the antineutrophil cytoplasmic antibody antigen proteinase 3. CD177 associates with β2 integrins and recognizes platelet endothelial cell adhesion molecule 1 (PECAM...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-03-768507
更新日期:2017-11-09 00:00:00
abstract::This study was performed to assess the utility of tumor-derived fragmentary DNA, or circulating tumor DNA (ctDNA), for identifying high-risk patients for relapse of acute myeloid leukemia and myelodysplastic syndrome (AML/MDS) after undergoing myeloablative allogeneic hematopoietic stem cell transplantation (alloSCT)....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-10-880690
更新日期:2019-06-20 00:00:00
abstract::Human hematopoietic progenitor cells express L-selectin and also express PSGL-1, a ligand for all selectins. Using a shear-based adhesion assay, a hematopoietic cell L-selectin ligand (HCLL) that is expressed on the hematopoietic cell line KG1a and on normal human hematopoietic progenitors was previously identified. T...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-10-15 00:00:00
abstract::Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is widely used in healthy donors for collection of peripheral blood progenitor cells (PBPCs) for allogeneic transplantation and granulocytes for transfusion. The spectrum of its biologic and molecular activities in healthy individuals is coming into sha...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2007-07-097543
更新日期:2008-02-15 00:00:00
abstract::The American Society of Hematology (ASH) turns 50 years old in 2008, and we have much to celebrate. Over those years the Society established its principles: to promote both the art and science of hematology and to hold a high-quality Educational Meeting. ASH membership has grown from a few hundred curious attendees at...
journal_title:Blood
pub_type: 历史文章,杂志文章
doi:10.1182/blood-2007-09-112326
更新日期:2008-01-01 00:00:00
abstract::T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignancy that accounts for ∼20% of ALL cases. Intensive chemotherapy regimens result in cure rates >85% in children and <50% in adults, warranting a search of novel therapeutic strategies. Although immune-based therapies have tremendously improved the trea...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003801
更新日期:2020-09-10 00:00:00
abstract::The cells of hematopoietic and vascular endothelial cell lineages are believed to share a common precursor, termed hemangioblast. However, the existence of a growth factor acting relatively specifically on hemangioblasts remains unclear. Here we report the identification of hemangiopoietin (HAPO), a novel growth facto...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-06-1825
更新日期:2004-06-15 00:00:00
abstract::A total of 124 patients who had survived at least 2 years after allogeneic bone marrow transplantation (BMT) were studied. Serum was collected at least once yearly. IgG antibodies were determined by enzyme-linked immunosorbent assay for measles and mumps. Rubella antibodies were analyzed by radial hemolysis. Antibody ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-07-15 00:00:00
abstract::An association between platelets, angiogenesis, and cancer has long been recognized, but the mechanisms linking them remains unclear. Platelets regulate new blood vessel growth through numerous stimulators and inhibitors of angiogenesis by several pathways, including differential exocytosis of angiogenesis regulators....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-334524
更新日期:2011-08-04 00:00:00
abstract::Pathologic blood clotting is a leading cause of morbidity and mortality in the developed world, underlying deep vein thrombosis, myocardial infarction, and stroke. Genetic predisposition to thrombosis is still poorly understood, and we hypothesize that there are many additional risk alleles and modifying factors remai...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-03-561027
更新日期:2014-07-03 00:00:00
abstract::Mutations in PIEZO1 are the primary cause of hereditary xerocytosis, a clinically heterogeneous, dominantly inherited disorder of erythrocyte dehydration. We used next-generation sequencing-based techniques to identify PIEZO1 mutations in individuals from 9 kindreds referred with suspected hereditary xerocytosis (HX) ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-05-786004
更新日期:2017-10-19 00:00:00
abstract::We describe the membrane localization of a new platelet-specific alloantigen, designated Naka, that is involved in refractoriness to HLA-matched platelet transfusions. By indirect immunoprecipitation, anti-Naka antibody precipitated a single, radiolabeled platelet membrane protein with a molecular weight (mol wt) of 9...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Varying doses of 5-azacytidine (5-aza) were given to four sickle cell individuals for 500, 200, 100, and 30 days. The percentage of fetal hemoglobin (HbF) containing reticulocytes (F reticulocytes) increased two- to five-fold within five days of 5-aza therapy in all patients, with a two- to three-fold rapid response (...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Both total metabolic tumor volume (TMTV), computed on baseline positron emission tomography (PET), and end of induction (EOI) PET are imaging biomarkers showing promise for early risk stratification in patients with high-tumor-burden follicular lymphoma. A model was built incorporating these 2 factors in 159 patients ...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1182/blood-2017-11-816298
更新日期:2018-05-31 00:00:00
abstract::Prophylactic fluconazole prevents candidiasis; however, this drug has no activity against molds. We performed a randomized trial to determine whether prophylactic itraconazole prevents invasive mold infections (IMIs). A total of 304 patients receiving allogeneic stem cell transplants (SCT) were randomized to receive f...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1182/blood-2003-08-2644
更新日期:2004-02-15 00:00:00
abstract::The cells and mechanisms involved in blood clot resorption are only partially known. We show that regulatory T (Treg) cells accumulate in venous blood clots and regulate thrombolysis by controlling the recruitment, differentiation and matrix metalloproteinase (MMP) activity of monocytes. We describe a clot Treg popula...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020005407
更新日期:2020-09-15 00:00:00
abstract::Twenty four samples of DNA from 23 unrelated individuals were analyzed for the presence of a novel restriction fragment length polymorphism (RFLP) involving the proto-oncogene ETS-1 at an Xba I site. Four samples from unrelated individuals lacked an Xba I site, giving rise to a longer restriction fragment detectable b...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::The treatment of multiple myeloma is considered a continuously evolving paradigm as a result of the growing availability of new and highly effective drugs, including first- and second-generation proteasome inhibitors, immunomodulatory agents, and monoclonal antibodies. Clinical trials advocate long-term rather than sh...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2017-01-725705
更新日期:2017-04-27 00:00:00
abstract::Studies with the granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin-3 (IL-3) fusion protein, PIXY321, demonstrated enhanced biological activity of this molecule in comparison with GM-CSF or IL-3 alone or in combination. Experiments were performed to study the mechanisms resulting in PIXY321-induced ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Endothelial cell-mediated coagulation and leukocyte adhesion are processes that might be connected by the generation of thrombin. To examine the interaction of procoagulant and proadhesive activity, cultures of endothelial cells were stimulated with tumor necrosis factor-alpha, which resulted in the surface expression...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-04-15 00:00:00
abstract::The epidemic form of the hemolytic uremic syndrome (HUS), beginning with an acute gastroenteritis, has been associated with a verocytotoxin-producing Escherichia coli infection. The endothelial cell is believed to play an important role in the pathogenesis of HUS. Endothelial cell damage by verocytotoxin-1 (VT-1) in v...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::The granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin (IL)-3/IL-5 receptor family regulates the production and function of myeloid cells. These cytokines signal through receptor complexes that consist of unique ligand-binding alpha-chains and common signaling beta-chains. IL-5 is distinct from IL-3...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-03-208850
更新日期:2009-10-29 00:00:00
abstract::We compared urokinase-type plasminogen activator (u-PA) in fluid phase and u-PA bound with its receptor on human blood monocytes with respect to proteolytic activity and susceptibility to inactivation by the plasminogen activator inhibitors PAI-1 and PAI-2. Receptor-bound u-PA is catalytically twice as efficient as fl...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00