Biologic and molecular effects of granulocyte colony-stimulating factor in healthy individuals: recent findings and current challenges.

abstract：:Heparin-induced thrombocytopenia (HIT) with thrombosis is a serious complication of heparin use. HIT sera can generate platelet-derived microparticles, which are produced in a heparin-dependent manner and are hypothesized to be important initial pathological participants because they promote vascular occlusion. To dat...

journal_title：Blood

authors： Hughes M,Hayward CP,Warkentin TE,Horsewood P,Chorneyko KA,Kelton JG

更新日期：2000-07-01 00:00:00

abstract：:Recent studies have identified several populations of progenitor cells in the human thymus. The hematopoietic precursor activity of these populations has been determined. The most primitive human thymocytes express high levels of CD34 and lack CD1a. These cells acquire CD1a and differentiate into CD4(+)CD8(+) through ...

journal_title：Blood

authors： Blom B,Verschuren MC,Heemskerk MH,Bakker AQ,van Gastel-Mol EJ,Wolvers-Tettero IL,van Dongen JJ,Spits H

更新日期：1999-05-01 00:00:00

abstract：:Alternatively activated macrophages (AAMϕ) are a major component of the response to helminth infection; however, their functions remain poorly defined. To better understand the helminth-induced AAMϕ phenotype, we performed a systems-level analysis of in vivo derived AAMϕ using an established mouse model. With next-gen...

journal_title：Blood

authors： Thomas GD,Rückerl D,Maskrey BH,Whitfield PD,Blaxter ML,Allen JE

更新日期：2012-12-13 00:00:00

abstract：:Neutrophil dysfunction consequent to influenza A virus infection has been described in vivo and in vitro and may contribute to the serious bacterial sequelae which occur in influenza-infected hosts. On the premise that such dysfunction may represent a form of "deactivation," we sought to characterize neutrophil activa...

journal_title：Blood

authors： Hartshorn KL,Collamer M,White MR,Schwartz JH,Tauber AI

更新日期：1990-01-01 00:00:00

abstract：:Gaucher disease, caused by a deficiency of the lysosomal enzyme glucocerebrosidase, leads to prominent glucosylceramide accumulation in lysosomes of tissue macrophages (Gaucher cells). Here we show glucosylsphingosine, the deacylated form of glucosylceramide, to be markedly increased in plasma of symptomatic nonneuron...

journal_title：Blood

authors： Dekker N,van Dussen L,Hollak CE,Overkleeft H,Scheij S,Ghauharali K,van Breemen MJ,Ferraz MJ,Groener JE,Maas M,Wijburg FA,Speijer D,Tylki-Szymanska A,Mistry PK,Boot RG,Aerts JM

更新日期：2011-10-20 00:00:00

abstract：:Erythropoietin-responsive stem cell (ERC) kinetics in anephric uremic rabbits were studied in vitro using the growth of erythroid colonies in a methyl cellulose system in cultures with and without the addition of erythropoietin (ESF). Approximately 68 hr after bilateral nephrectomy, an increase in BUN and decreases in...

journal_title：Blood

authors： Moriyama Y,Fisher JW

更新日期：1975-05-01 00:00:00

abstract：:We studied the cell kill induced by granulocyte-macrophage colony-stimulating factor (GM-CSF ) fused to Diphtheria Toxin (DT-GM-CSF ) in acute myeloid leukemia (AML) samples and in populations of normal primitive hemopoietic progenitor cells. AML samples from three patients were incubated in vitro with 100 ng/mL DT-GM...

journal_title：Blood

authors： Terpstra W,Rozemuller H,Breems DA,Rombouts EJ,Prins A,FitzGerald DJ,Kreitman RJ,Wielenga JJ,Ploemacher RE,Löwenberg B,Hagenbeek A,Martens AC

更新日期：1997-11-01 00:00:00

abstract：:The regulation of megakaryocytopoiesis and thrombopoiesis appears to be under the control of an array of hematopoietic growth factors. To determine the relationship of endogenous thrombopoietic cytokine levels and circulating platelet (PLT) counts, we measured the levels of thrombo-poietin (TPO), interleukin-11 (IL-11...

journal_title：Blood

authors： Chang M,Suen Y,Meng G,Buzby JS,Bussel J,Shen V,van de Ven C,Cairo MS

更新日期：1996-11-01 00:00:00

abstract：:We genotyped 370 subjects with primary myelofibrosis (PMF) and 148 with postpolycythemia vera/postessential thrombocythemia (PPV/PET) MF for mutations of EZH2. Mutational status at diagnosis was correlated with hematologic parameters, clinical manifestations, and outcome. A total of 25 different EZH2 mutations were de...

journal_title：Blood

authors： Guglielmelli P,Biamonte F,Score J,Hidalgo-Curtis C,Cervantes F,Maffioli M,Fanelli T,Ernst T,Winkelman N,Jones AV,Zoi K,Reiter A,Duncombe A,Villani L,Bosi A,Barosi G,Cross NC,Vannucchi AM

更新日期：2011-11-10 00:00:00

abstract：:Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed t...

journal_title：Blood

authors： Gunji Y,Nakamura M,Hagiwara T,Hayakawa K,Matsushita H,Osawa H,Nagayoshi K,Nakauchi H,Yanagisawa M,Miura Y

更新日期：1992-07-15 00:00:00

abstract：:Peroxiredoxin 2 (Prx2), a thiol-dependent peroxidase, is the third most abundant protein in the erythrocyte, and its absence in knock-out mice gives rise to hemolytic anemia. We have found that in human erythrocytes, Prx2 was extremely sensitive to oxidation by H(2)O(2), as dimerization was observed after exposure of ...

journal_title：Blood

authors： Low FM,Hampton MB,Peskin AV,Winterbourn CC

更新日期：2007-03-15 00:00:00

abstract：:We have generated a large, unique database that includes morphologic, clinical, cytogenetic, and follow-up data from 2124 patients with myelodysplastic syndromes (MDSs) at 4 institutions in Austria and 4 in Germany. Cytogenetic analyses were successfully performed in 2072 (97.6%) patients, revealing clonal abnormaliti...

journal_title：Blood

authors： Haase D,Germing U,Schanz J,Pfeilstöcker M,Nösslinger T,Hildebrandt B,Kundgen A,Lübbert M,Kunzmann R,Giagounidis AA,Aul C,Trümper L,Krieger O,Stauder R,Müller TH,Wimazal F,Valent P,Fonatsch C,Steidl C

更新日期：2007-12-15 00:00:00

abstract：:Idiopathic pneumonia syndrome (IPS) is a relatively common, frequently fatal clinical entity, characterized by noninfectious acute lung inflammation following allogeneic stem cell transplantation (SCT), the mechanisms of which are unclear. In this study, we demonstrate that immune suppression with cyclosporin after SC...

journal_title：Blood

authors： Varelias A,Gartlan KH,Kreijveld E,Olver SD,Lor M,Kuns RD,Lineburg KE,Teal BE,Raffelt NC,Cheong M,Alexander KA,Koyama M,Markey KA,Sturgeon E,Leach J,Reddy P,Kennedy GA,Yanik GA,Blazar BR,Tey SK,Clouston AD,MacDon

更新日期：2015-04-09 00:00:00

abstract：:The serine protease prolylcarboxypeptidase (PRCP), isolated from human umbilical vein endothelial cells (HUVECs), is a plasma prekallikrein (PK) activator. PRCP cDNA was cloned in pMT/BIP/V5-HIS-C, transfected into Schneider insect (S2) cells, and purified from serum-free media. Full-length recombinant PRCP (rPRCP) ac...

journal_title：Blood

authors： Shariat-Madar Z,Mahdi F,Schmaier AH

更新日期：2004-06-15 00:00:00

abstract：:The FOXP1 forkhead transcription factor is targeted by recurrent chromosome translocations in several subtypes of B-cell non-Hodgkin lymphomas, where high-level FOXP1 protein expression has been linked to a poor prognosis. Western blotting studies of diffuse large B-cell lymphoma (DLBCL) cell lines unexpectedly identi...

journal_title：Blood

authors： Brown PJ,Ashe SL,Leich E,Burek C,Barrans S,Fenton JA,Jack AS,Pulford K,Rosenwald A,Banham AH

更新日期：2008-03-01 00:00:00

abstract：:Caloric restriction (CR) is proposed to decrease tumorigenesis through a variety of mechanisms including effects on glycolysis. However, the understanding of how CR affects the response to cancer therapy is still rudimentary. Here, using the Eµ-Myc transgenic mouse model of B-cell lymphoma, we report that by reducing ...

journal_title：Blood

authors： Meynet O,Zunino B,Happo L,Pradelli LA,Chiche J,Jacquin MA,Mondragón L,Tanti JF,Taillan B,Garnier G,Reverso-Meinietti J,Mounier N,Michiels JF,Michalak EM,Carles M,Scott CL,Ricci JE

更新日期：2013-10-03 00:00:00

abstract：:The high frequency of allogeneic reactive CD8(+) T cells in human and their resistance to immunosuppression might be one of the reasons why successful tolerance-inducing strategies in rodents have failed in primates. Studies on the requirement for T-helper cells in priming CD8(+) T-cell responses have led to disparate...

journal_title：Blood

authors： Lemoine R,Velge-Roussel F,Herr F,Felix R,Nivet H,Lebranchu Y,Baron C

更新日期：2010-10-21 00:00:00

abstract：:In this issue of Blood, Smith and colleagues report on the functional role of the interaction between these 2 proteins by studying the involved binding sites responsible for clot stabilization.(1) ...

journal_title：Blood

authors： Kohler HP

更新日期：2013-03-14 00:00:00

abstract：:Autoimmune lymphoproliferative syndrome (ALPS) is a disorder of abnormal lymphocyte survival caused by defective Fas-mediated apoptosis, leading to lymphadenopathy, hepatosplenomegaly, and an increased number of double-negative T cells (DNTs). Treatment options for patients with ALPS are limited. Rapamycin has been sh...

journal_title：Blood

authors： Teachey DT,Obzut DA,Axsom K,Choi JK,Goldsmith KC,Hall J,Hulitt J,Manno CS,Maris JM,Rhodin N,Sullivan KE,Brown VI,Grupp SA

更新日期：2006-09-15 00:00:00

abstract：:We have verified the hypothesis that multiple myeloma (MM) may be disseminated by circulating clonogenic cells that selectively home to the bone marrow (BM) to receive the signal(s) leading to proliferation, terminal differentiation, and production of the osteoclast activating factors. Long-term cultures of stromal ce...

journal_title：Blood

authors： Caligaris-Cappio F,Bergui L,Gregoretti MG,Gaidano G,Gaboli M,Schena M,Zallone AZ,Marchisio PC

更新日期：1991-06-15 00:00:00

abstract：:Hydroxyurea treatment of sickle cell mice improved their survival from pneumococcal pneumonia, counteracting the abnormally elevated inflammatory response and reducing invasion of bacteria into the bloodstream, through down regulation of E-selectin. ...

journal_title：Blood

authors： Hsu LL

更新日期：2012-02-23 00:00:00

abstract：:In this issue of Blood, Doyle et al provide evidence that knockout of the genes encoding the two most abundant eosinophil secondary granule proteins disrupts the normal differentiation of eosinophils from progenitors in the bone marrow, providing a novel strain of mice with a highly specific deficiency in eosinophilop...

journal_title：Blood

authors： Ackerman SJ

更新日期：2013-08-01 00:00:00

abstract：:Epinephrine causes platelet aggregation and secretion by interacting with alpha 2-adrenergic receptors on the platelet surface. Platelet aggregation requires the binding of fibrinogen to a specific receptor on the membrane glycoprotein IIb-IIIa complex. Although the IIb-IIIa complex is identifiable on the surface of r...

journal_title：Blood

authors： Shattil SJ,Motulsky HJ,Insel PA,Flaherty L,Brass LF

更新日期：1986-12-01 00:00:00

abstract：:The expression of Lewis fucosyltransferase (FT) mRNA was examined in gastric mucosa from two Lewis-positive [Le(+)] and two Lewis-negative [Le(-)] individuals. Northern blot analysis demonstrated that levels of mRNA were similar in both Le(+) and Le(-) gastric mucosa. We isolated the protein-coding region of the Lewis...

journal_title：Blood

authors： Koda Y,Kimura H,Mekada E

更新日期：1993-11-01 00:00:00

abstract：:The origin of low-grade mucosa-associated lymphoid tissue (MALT)-type B-cell lymphoma is still unclear. Using a novel two-step procedure, we have sequenced the Ig VH genes expressed by cells from four patients with gastric low-grade MALT-type lymphoma. The nucleotide sequences of the complementarity determining region...

journal_title：Blood

authors： Qin Y,Greiner A,Trunk MJ,Schmausser B,Ott MM,Müller-Hermelink HK

更新日期：1995-11-01 00:00:00

abstract：:Monoclonal gammopathy of undetermined significance (MGUS) is present in ∼2% of individuals age >50 years. The increased risk of multiple myeloma (MM) in relatives of individuals with MGUS is consistent with MGUS being a marker of inherited genetic susceptibility to MM. Common single-nucleotide polymorphisms (SNPs) at ...

journal_title：Blood

authors： Weinhold N,Johnson DC,Rawstron AC,Försti A,Doughty C,Vijayakrishnan J,Broderick P,Dahir NB,Begum DB,Hosking FJ,Yong K,Walker BA,Hoffmann P,Mühleisen TW,Langer C,Dörner E,Jöckel KH,Eisele L,Nöthen MM,Hose D,Davies

更新日期：2014-04-17 00:00:00

abstract：:Reestablishment of competent regulatory pathways has emerged as a strategy to reduce the severity of graft-versus-host disease (GVHD), and recalibrate the effector and regulatory arms of the immune system. However, clinically feasible, cost-effective strategies that do not require extensive ex vivo cellular manipulati...

journal_title：Blood

authors： Belle L,Agle K,Zhou V,Yin-Yuan C,Komorowski R,Eastwood D,Logan B,Sun J,Ghilardi N,Cua D,Williams CB,Gaignage M,Marillier R,van Snick J,Drobyski WR

更新日期：2016-10-20 00:00:00

abstract：:The transcription factor TAL1 has major functions during embryonic hematopoiesis and in adult erythropoiesis and megakaryocytopoiesis. These functions rely on different TAL1 structural domains that are responsible for dimerization, transactivation, and DNA binding. Previous work, most often done in mice, has shown tha...

journal_title：Blood

authors： Ravet E,Reynaud D,Titeux M,Izac B,Fichelson S,Roméo PH,Dubart-Kupperschmitt A,Pflumio F

更新日期：2004-05-01 00:00:00

abstract：:Natural killer cells (NK) were studied to determine factors important in their expansion. Flourescence-activated cell sorter (FACS) purified CD56+/CD3- NK cells cultured alone for 18 days in rIL-2 containing medium (1,000 U/mL) showed enhanced cytotoxicity but only minimal expansion. NK expansion was increased (12.5 +...

journal_title：Blood

authors： Pierson BA,Gupta K,Hu WS,Miller JS

更新日期：1996-01-01 00:00:00

abstract：:The cell-surface receptor c-kit and its cognate ligand stem-cell factor (SCF) or steel factor (SLF) are important for the maintenance of hematopoiesis both in vitro and in vivo. Transforming growth factor-beta (TGF-beta) has been shown to be a potent inhibitor of SLF-mediated synergistic growth of murine Lin-Sca-1+ pr...

journal_title：Blood

authors： Dubois CM,Ruscetti FW,Stankova J,Keller JR

更新日期：1994-06-01 00:00:00