Abstract：
:Apoptosis is a mode of cell death that is carefully regulated based on cellular and environmental signals. The ability to modulate the individual cellular machinery and thereby to promote apoptosis is an important strategy in cancer therapy. It has previously been shown that overexpression of the transcription factor E2F-1 can induce apoptosis in quiescent rat embryo fibroblasts. This effect has been reported to occur in a p53-dependent manner. To investigate whether overexpression of E2F-1 could also induce apoptosis in human cancer cells, a recombinant adenovirus vector containing the transgene E2F-1 under control of the cytomegalovirus promoter (Ad5CMVE2F) was used to induce high levels of the E2F-1 protein in human breast and ovarian carcinoma cell lines. Significant morphological changes occurred in four of the five cell lines within 48 h of transduction with the Ad5CMVE2F. These changes were consistent with apoptosis, which was confirmed further by DNA fragmentation assay and fluorescence-activated cell sorting analysis. On the basis of these assays, which show that apoptosis occurred in those cell lines with mutations in the p53 gene, we suggest that the induction of E2F-1-mediated apoptosis does not require wild-type p53 when E2F-1 is overexpressed using an adenovirus-based strategy.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Hunt KK,Deng J,Liu TJ,Wilson-Heiner M,Swisher SG,Clayman G,Hung MCsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
4722-6issue
21eissn
0008-5472issn
1538-7445journal_volume
57pub_type
杂志文章相关文献
CANCER RESEARCH文献大全abstract：:We report that tumor necrosis factor (TNF) alpha induced a strong antitumor immune reaction when it was produced in arteries leading to tumors by gene transfer in vivo. We used a mouse model carrying a sarcoma-180 tumor in the right footpad and injected the fusogenic liposomes encapsulating the human TNF-alpha gene in...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1998-12-15 00:00:00
abstract：:The expression of cytochrome P450 genes directly within target cells is an important determinant of human susceptibility to cancers, birth defects, and other chemically initiated diseases. One pivotal gene, CYP1A1, codes for an inducible cytochrome P450 isozyme (P450IA1) responsible for the bioactivation of numerous c...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-07-15 00:00:00
abstract：:Maximum rates of nitrofuran reduction by intact mammalian cells and homogenates of mouse liver are obtained under anaerobic conditions, although significant reduction does occur with gas mixtures containing 5% oxygen and less. Single-strand breaks in DNA, measured as a decrease in the sedimentation constant on alkalin...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:Bleomycin treatment produced dose-dependent changes in lung collagen content and in several measurable histopathological parameters. NIH/Swiss mice were treated twice weekly for 6 weeks with bleomycin, 0, 1, 20, or 40 mg/kg s.c. The two highest doses produced mortalities of 35 and 100%, respectively, as well as loss o...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:Salvage of preformed nucleosides requires transport across the plasma membrane by sodium-dependent (concentrative) and sodium-independent (equilibrative) mechanisms. These transport systems are also the route of cellular uptake for nucleoside analogues, including gemcitabine (2',2'-difluorodeoxycytidine), a deoxycytid...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:We have performed isobologram analyses of the ability of tamoxifen (TAM) to alter the response to Adriamycin (ADR) and vinblastine (VBL) in human breast cancer cells. MCF-7 cells express functional receptors for estrogen and progesterone but do not express detectable levels of M(r) 170,000 glycoprotein (gp170). CL 10....
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1994-01-15 00:00:00
abstract：:Cytosol estradiol receptor from MTW9-D (ovarian dependent) and MTW9-MtT (ovarian independent) rat mammary tumors were fractionated by chromatofocusing, a procedure which separates proteins on an ion-exchange column as a function of isoelectric point. Receptor from MTW9-D usually fractionated as three peaks with mean p...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Two murine monoclonal antibodies, 2H6G5 (IgM) and 4A9E10 (IgG3), were obtained by using either cultured human hepatocellular carcinoma cells (PLC/PRF/5) or the acidic glycolipid mixture prepared from the same cells as immunogens. The antigen in PLC/PRF/5 cell membranes recognized by both antibodies was identified as a...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:We know that the Yin Yang 1 protein (YY1) overexpression is positively and strongly correlated with the degree of malignancy of bone tumors. Therefore, we questioned whether we could influence cell invasiveness by deleting YY1 in human osteosarcoma cells (SaOs-2), as tested in Matrigel-coated filters and metastasis im...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-07-5582
更新日期：2008-03-15 00:00:00
abstract：:The gene of phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) has been implicated as an oncogene in ovarian cancer [L. Shayesteh et al., Nat. Genet., 21: 99-102, 1999]. In this study, we examined the expression of PIK3CA mRNA and its p110alpha protein product in human ovarian carcinoma and investigated it...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2003-07-15 00:00:00
abstract：:Ovarian and testicular tumors were induced in the offspring (F1 generation) of MRC rats that received single or multiple doses of N-nitrosobis(2-oxopropyl)amine on the 14th, 18th, and/or 20th days of pregnancy. The ovarian tumor incidence was significantly higher (46%) in the F1 generation exposed to the carcinogen a ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:The fusion transcript AML1/ETO was detected in the bone marrow of two t(8;21)-negative acute myelogenous leukemia (AML) patients by means of reverse transcription-polymerase chain reaction. This fusion transcript is identical to the one transcribed from the t(8;21) translocation base, as deduced from (a) the size and ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Tumor-specific immune tolerance limits the effectiveness of cancer vaccines. In addition, tumor vaccines alone have a limited potential for the treatment of measurable tumor burdens. This highlights the importance of identifying more potent cancer vaccine strategies for clinical testing. We tested immune-modulating do...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:An assay using a bimane derivative has been developed to detect free glutathione (GSH) in individual viable cells by flow cytometry. Monochlorobimane [syn-(ClCH2CH3)-1,5-diazabicycla[3.30]acta-3,6-diene-2,8-dio ne], itself nonfluorescent, reacts with GSH to form a highly fluorescent derivative. High pressure liquid ch...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Currently, no curative therapy for metastatic prostate cancer exists. Causing prostate cancer cells to express functionally active sodium iodide symporter (NIS) would enable those cells to concentrate iodide from plasma and might offer the ability to treat prostate cancer with radioiodine. Therefore, the aim of our st...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:The Adenomatous polyposis coli (Apc) gene is mutated in up to 80% of sporadic colorectal cancers. After Apc loss, there is deregulation of the Wnt signaling pathway and transactivation of T-cell factor/leukemia enhancing factor target genes such as C-Myc. This review focuses on recent data highlighting the importance ...
journal_title：Cancer research
pub_type： 杂志文章,评审
doi：10.1158/0008-5472.CAN-07-5558
更新日期：2008-07-01 00:00:00
abstract：:We examined the role of constitutional genetic variation at the UDP-glucuronosyltransferase (UGT) 1A1 locus in breast cancer susceptibility. The UGT1A1 enzyme is a major UGT involved in estradiol glucuronidation. To date, four UGT1A1 variant alleles characterized by a variation in the number of TA from five through ei...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-02-15 00:00:00
abstract：:Small hydrophobic peptides were studied as possible substrates of the multidrug resistance protein (MRP)-1 (ABCC1) transmembrane transporter molecule. As observed earlier for P-glycoprotein- (Pgp; ABCB1) overexpressing cells, MRP1-overexpressing cells, including cells stably transfected with the MRP1 cDNA, showed dist...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2001-03-15 00:00:00
abstract：:Previously we described a human B-lymphoma xenotransplantation model in which the immunotherapeutic activity of monoclonal antibodies (mAbs), directed against human B-cell antigens, can be studied. An anti-CD19 mAb of IgG2a subclass was therapeutically active by itself in this model, and its efficacy was increased by ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-09-15 00:00:00
abstract：:The tumor localization of various anti-CD5 immunotoxins (ITs) was studied in vivo in BALB/c nude mice bearing human ascitic Ichikawa tumor cells. Plasma and ascitic clearance of IT as well as the number of intact IT molecules taken up per tumor cell was measured with a highly sensitive immunoenzymometric assay. This a...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-05-15 00:00:00
abstract：:Melanoma differentiation associated gene-7/interleukin-24 (Mda-7/IL-24), a novel member of the IL-10 family of cytokines, uniquely displays cancer-specific apoptosis-inducing activity. Positive results in ongoing phase I/II clinical trials have strengthened the possibility of its utilization as a cancer gene therapeut...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.can-04-0200
更新日期：2004-05-01 00:00:00
abstract：:Apoptosis is a critical early cellular event in the development of polycystic kidney disease (PKD) in humans and mice. In the SBM transgenic model of PKD, both apoptosis and proliferation are c-myc driven and are independent of p53 and Bcl-2 pathways. On the basis of recent evidence implicating the FasL/Fas pathway in...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2002-04-15 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSC) represent a primary mechanism of immune evasion in tumors and have emerged as a major obstacle for cancer immunotherapy. The immunoinhibitory activity of MDSC is tightly regulated by the tumor microenvironment and occurs through mechanistic mediators that remain unclear. Here, w...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-19-0880
更新日期：2019-10-01 00:00:00
abstract：:Adaptive therapy seeks to exploit intra-tumoral competition to avoid, or at least delay, the emergence of therapy resistance in cancer. Motivated by promising results in prostate cancer, there is growing interest in extending this approach to other neoplasms. As such, it is urgent to understand the characteristics of ...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-20-0806
更新日期：2020-11-10 00:00:00
abstract：:Focal adhesion kinase (FAK) functions downstream of integrins and growth factor receptors to promote tumor cell motility and invasion. In colorectal cancer, FAK is activated by amidated gastrin, a protumorigenic hormone. However, it is unclear how FAK receives signals from the gastrin receptor or other G-protein-coupl...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-10-2894
更新日期：2011-01-15 00:00:00
abstract：:The effects of the dopamine agonist bromocriptine and antagonist haloperidol on the incidence and histology of colon tumors induced by azoxymethane and on the labeling index of colon mucosa were investigated in Wistar rats. Rats received weekly s.c. injections of 7.4 mg/kg of body weight azoxymethane for 10 weeks and ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1991-11-15 00:00:00
abstract：:Helicobacter pylori-induced gastritis is the strongest singular risk factor for gastric adenocarcinoma. Matrix metalloproteinase-7 (MMP-7) is a proteolytic enzyme that can modify the intestinal microbial replicative niche as well as affect tumorigenesis, and H. pylori stimulates expression of MMP-7 in gastric epitheli...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-09-2899
更新日期：2010-01-01 00:00:00
abstract：:Convection enhanced delivery (CED) is potentially a powerful method to improvethe targeting of macromolecules to the central nervous system by applying a pressure gradient to establish bulk flow through the brain interstitium during infusion. The purpose of the present study was to evaluate CED as a means to improve t...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-15 00:00:00
abstract：:Patients suffering from bone metastases of follicular thyroid carcinoma (FTC) have a poor prognosis because of the lack of effective treatment strategies. The overexpression of epidermal growth factor receptor (EGFR) associated with increased vascularity has been implicated in the pathogenesis of FTC and subsequent bo...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-04-4196
更新日期：2005-06-01 00:00:00
abstract：:A major goal of cancer research is to match specific therapies to molecular targets in cancer. Genome-scale expression profiling has identified new subtypes of cancer based on consistent patterns of variation in gene expression, leading to improved prognostic predictions. However, how these new genetic subtypes of can...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-07-0382
更新日期：2008-01-15 00:00:00
