Aberrations of the K-ras, p53, and APC genes in extrahepatic bile duct cancer.

Abstract:

BACKGROUND AND OBJECTIVES:The genetic alterations involved in extrahepatic bile duct (EHBD) cancer are poorly understood. Our aim was to identify aberrations of the K-ras, p53, and APC genes in EHBD cancer. METHODS:We investigated aberrations of these genes in 52 EHBD cancers using polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, followed by direct sequence determination and a PCR restriction fragment length polymorphism assay. RESULTS:The K-ras, p53, and APC genes were mutated in 9.6%, 32.7%, and 0% of EHBD cancers, respectively. Loss of heterozygosity at the p53 and APC gene loci was identified in 15.6% and 38.5% of EHBD cancers, respectively. CONCLUSIONS:Our results suggest that an unknown suppressor gene on 5q other than the APC gene may be responsible for EHBD cancer.

journal_name

J Surg Oncol

authors

Suto T,Habano W,Sugai T,Uesugi N,Funato O,Kanno S,Saito K,Nakamura Si

doi

10.1002/(sici)1096-9098(200003)73:3<158::aid-jso9>

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

158-63

issue

3

eissn

0022-4790

issn

1096-9098

pii

10.1002/(SICI)1096-9098(200003)73:3<158::AID-JSO9>

journal_volume

73

pub_type

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