Augmentation of lymphoproliferative activity of tumor-bearing BALB/c mice to soluble tumor antigens by surgical manipulation: the possible role of macrophages.

Abstract:

:Lymphoproliferative activity was assessed by 3H-thymidine incorporation in spleen cells of mammary tumor bearing BALB/c mice, mice which had their tumors surgically removed, and mice with sham surgery after stimulation in vitro with a soluble extract of syngeneic tumor antigens. Surgical removal of tumors caused an average of 65% decline in reactivity at 5 days postoperative as compared to reactivity of animals with their tumors intact. Sham surgery caused an average of 33% elevation in reactivity. Macrophages added to cultures at spleen cell/macrophage ratio of 100:1 caused an average 171%, 59%, and 12% elevation in the activity of surgery, tumor-bearing, and sham-surgery animals, respectively. Surgical manipulation of tumor-bearing mice, therefore, augmented in situ the ability of spleen cells to exhibit lymphoproliferative activity to a soluble syngeneic antigenic extract in vitro, possibly by a mechanism involving the activation of macrophages and requiring the presence of a sizable antigenic load.

journal_name

J Surg Oncol

authors

Laing CA,Japal KM,Baker ME

doi

10.1002/jso.2930350102

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

1-6

issue

1

eissn

0022-4790

issn

1096-9098

journal_volume

35

pub_type

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