Abstract:
:In the present research, we studied the effect of the administration of melatonin or S-adenosyl-L-methionine (S-AMe) on oxidative stress and hepatic cholestasis produced by double ligature of the extra-hepatic biliary duct (LBD) in adult male Wistar rats. Hepatic oxidative stress was evaluated by the changes in the amount of lipid peroxides and by the reduced glutathione content (GSH) in lysates of erythrocytes and homogenates of hepatic tissue. The severity of the cholestasis and hepatic injury were determined by the changes in the plasma enzyme activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP), g-glutamyl-transpeptidase (GGT), and levels of albumin, total bilirubin (TB) and direct bilirubin (DB). Either melatonin or S-AMe were administered daily 3 days before LBD, and for 10 days after biliary obstruction. LDB caused highly significant increases in plasma enzyme activities and in bilirubin and lipid peroxides levels in erythrocytes and hepatic tissue. At the same time, this procedure produced a notable decrease in the GSH pools in these biological media. Both melatonin and S-AMe administration were effective as antioxidants and hepatoprotective substances, although the protective effects of melatonin were superior; it prevented the GSH decrease and reduced significantly the increases in enzyme activities and lipid peroxidation products produced by biliary ligature. S-AMe did not modify the increased GGT activity nor did it decrease greatly the TB levels (43% melatonin vs. 14% S-AMe). However, S-AMe was effective in preventing the loss of GSH in erythrocytes and hepatic tissue, as was melatonin. The obtained data permit the following conclusions. First, the LDB models cause marked hepatic oxidative stress. Second, the participation of free radicals of oxygen in the pathogenecity and severity of cholestasis produced by the acute obstruction of the extra-hepatic biliary duct is likely. Third, the results confirm the function of S-AMe as an antioxidant and hepatoprotector. Finally, melatonin is far more potent and provides superior protection as compared to S-AMe. Considering the decrease in oxidative stress and the intensity of cholestasis, these findings have interesting clinical implications for melatonin as a possible therapeutic agent in biliary cholestasis and parenchymatous liver injury.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
López PM,Fiñana IT,De Agueda MC,Sánchez EC,Muñoz MC,Alvarez JP,De La Torre Lozano EJdoi
10.1034/j.1600-079x.2001.280303.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
143-9issue
3eissn
0742-3098issn
1600-079Xjournal_volume
28pub_type
杂志文章abstract::The effect of light on melatonin biosynthesis was examined in detached rice (Oryza sativa cv. Asahi) leaves during the senescence process. The detached leaves were exposed to senescence treatment either in constant darkness or in constant light, and subjected to HPLC analysis for melatonin and its precursors. Higher m...
journal_title:Journal of pineal research
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doi:10.1111/j.1600-079X.2012.00976.x
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abstract::Melatonin was tested in an ongoing attempt to find the endogenous antagonists of quinolinic acid, an endogenous convulsant. Among a great number of metabolites that have been tried before, only a few were found (cerulein and quinaldic acid in mice and kynurenic acid in rats). In SHR (bred from Swiss) male mice, intrac...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1998.tb00535.x
更新日期:1998-05-01 00:00:00
abstract::Possible role of melatonin in the germination of negatively photoblastic and thermosensitive seeds of Phacelia tanacetifolia Benth was studied. Final germination percentage (FGP) was determined in the presence or absence of light at various temperatures, ranging from 0 to 40°C. The highest FGP was determined as 48.7% ...
journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12230
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1998.tb00367.x
更新日期:1998-01-01 00:00:00
abstract::The purpose of this study was to determine and compare the effects of chronic melatonin treatment in vivo on several aspects of prolactin (PRL) cell activity (PRL synthesis, storage, and release) in vitro in male and female Syrian hamsters. Adult male and female hamsters were maintained on long photoperiod and treated...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1989.tb00446.x
更新日期:1989-01-01 00:00:00
abstract::Maternal hyperthermia induces early embryonic death via increased oxidative stress to the embryo. In this study, we examined whether melatonin administered to heat-stressed pregnant mice would reduce hyperthermia-induced embryonic death. Mice were heat stressed (12 hr at 35 degrees C, 60% relative humidity) on the day...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2005.00260.x
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1989.tb00911.x
更新日期:1989-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2011.00971.x
更新日期:2012-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1996.tb00266.x
更新日期:1996-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2005.00287.x
更新日期:2006-03-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1986.tb00720.x
更新日期:1986-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1989.tb00426.x
更新日期:1989-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12120
更新日期:2014-04-01 00:00:00
abstract::Melatonin is involved in the control of various physiological functions, such as sleep, cell growth and free radical scavenging. The ability of melatonin to behave as an antioxidant, together with the fact that the Alzheimer-related amyloid β-peptide (Aβ) triggers oxidative stress through hydroxyl radical-induced cell...
journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1992.tb00039.x
更新日期:1992-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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abstract::Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell-to-cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature-, time-, and pH-dependent binding site for glutama...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1993.tb00483.x
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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更新日期:2011-03-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Journal of pineal research
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更新日期:2013-11-01 00:00:00
abstract::The circadian system comprises several peripheral oscillators and a central rhythm generator that, in mammals, is located in the suprachiasmatic nucleus of the hypothalamus. Expression of clock genes is a characteristic feature of the central rhythm generator and the peripheral oscillators. With regard to the rhythmic...
journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
abstract::We evaluated the antioxidant property of melatonin as it relates to the vasospastic effect of hydrogen peroxide (H2O2) on the human umbilical artery. Helical sections of umbilical arteries were obtained from healthy pregnant women who were delivered between weeks 37 and 39 of gestation. Changes in maximal potassium ch...
journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-04-01 00:00:00