Abstract:
:Earlier studies showed that melatonin reduced the growth of 17-beta-estradiol (E(2))-induced rat pituitary prolactin-secreting tumor (prolactinoma) in vivo. The mechanisms of melatonin's inhibitory action on the prolactin-secreting tumor were further explored by investigating the in vitro effects of melatonin on the growth of pituitary prolactin-secreting tumor cells. Primary cultured prolactinoma cells from E(2)-induced rat pituitary prolactin-secreting tumor were treated with 10(-5), 10(-4) or 10(-3) m melatonin for 5 days. Apoptosis was evaluated using flow cytometry and the TdT-mediated dUTP nick-end labeling (TUNEL) method. In addition, cell viability was analyzed by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. It was found that incubation of prolactinoma cells with 10(-5), 10(-4) or 10(-3) m melatonin for 5 days inhibited cell growth and increased cell apoptosis. Furthermore, melatonin increased caspase-3 activity, Bax mRNA expression, and cytochrome c protein expression. Conversely, Bcl-2 mRNA expression and mitochondrial membrane potential were inhibited by melatonin treatment. Our results further suggest that melatonin inhibits tumor growth by inducing apoptosis of rat pituitary prolactin-secreting tumor directly via the damage of mitochondria.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
Yang QH,Xu JN,Xu RK,Pang SFdoi
10.1111/j.1600-079X.2006.00403.xsubject
Has Abstractpub_date
2007-03-01 00:00:00pages
172-9issue
2eissn
0742-3098issn
1600-079Xpii
JPI403journal_volume
42pub_type
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1600-079X.2010.00850.x
更新日期:2011-04-01 00:00:00
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journal_title:Journal of pineal research
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doi:10.1111/j.1600-079X.2005.00306.x
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journal_title:Journal of pineal research
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1999.tb00619.x
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2010.00819.x
更新日期:2011-03-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00424.x
更新日期:2007-04-01 00:00:00
abstract::Early studies on the physiological effects of melatonin typically reported hypnotic 'side-effects'. Later studies, specifically addressing this action, failed to reliably replicate hypnotic effects using standard polysomnography. This difference may be related to differences in the basic physiological action of melato...
journal_title:Journal of pineal research
pub_type: 杂志文章,评审
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更新日期:1993-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00506.x
更新日期:2008-03-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12126
更新日期:2014-04-01 00:00:00
abstract::The objective of this study was to determine the role of alpha-adrenoceptors in melatonin production by rat pineal gland. Pineal glands were isolated from adult male rats and maintained in organ baths. The perfusate was sampled every 5 min, stored, and later assayed for melatonin. Exposure to norepinephrine (10 microM...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1034/j.1600-079x.2002.01842.x
更新日期:2002-05-01 00:00:00
abstract::Day and nighttime melatonin production in the pineal gland was compared in normal and cardiomyopathic (polydystrophic) adult male Syrian hamsters. These strains of hamsters were selected for comparison because the cardiomyopathetic hamster displays a deficient transmembrane Ca(2+)-pump in a number of tissues, and intr...
journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00