Abstract：
:N-acetylserotonin methyltransferase (ASMT) is the final enzyme in a biosynthetic pathway that produces melatonin. ASMT may play a rate-limiting role in the production of this bioactive molecule in plants. There are three ASMT genes (ASMT1-ASMT3) in the rice genome, but only ASMT1 has been functionally characterized. A major barrier to further progress in characterizing these genes has been a failure of functional expression within the Escherichia coli. Purified recombinant ASMT2 and ASMT3 are inactive in ASMT enzyme catalysis. To determine the biological functions of ASMT2 and ASMT3, we first overexpressed them in rice calli, which resulted in enhanced production of melatonin in the respective transgenic calli. To further corroborate the functions of ASMT2 and ASMT3 as ASMT genes, we generated stable transgenic rice plants. ASMT enzyme activity was increased in comparison with the wild type in T2 homozygous transgenic rice plants expressing three ASMT genes independently. When seedlings were treated with 1 mm N-acetylserotonin (NAS), leaf melatonin contents were higher in the three transgenic lines than in the wild type. There were no significant differences between the transgenic lines and the wild type without this treatment. ASMT1 and ASMT2 transcripts were highly expressed in stems and flowers, but ASMT3 was barely detectable in any of the plant organs. All three ASMT mRNAs were simultaneously induced in treatments with abscisic and methyl jasmonic acids.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
Park S,Byeon Y,Back Kdoi
10.1111/jpi.12088subject
Has Abstractpub_date
2013-11-01 00:00:00pages
409-15issue
4eissn
0742-3098issn
1600-079Xjournal_volume
55pub_type
杂志文章abstract：:Rat pineal hydroxyindole-O-methyltransferase (HIOMT; EC 2.1.1.4) was purified by affinity chromatography using an S-adenosyl-L-homocysteine agarose column. This single-step procedure, which is rapid, simple, and applicable to small quantities of tissue, gave a large enrichment of a protein (Mr approximately 38,000) id...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1986.tb00761.x
更新日期：1986-01-01 00:00:00
abstract：:The aim was to examine the effect of perioperative melatonin treatment on clinical cardiac morbidity and markers of myocardial ischemia in patients undergoing elective surgery for abdominal aortic aneurism. Reperfusion injury results in increased cardiac morbidity in patients undergoing surgery for abdominal aortic an...
journal_title：Journal of pineal research
pub_type： 杂志文章,随机对照试验
doi：10.1111/jpi.12138
更新日期：2014-08-01 00:00:00
abstract：:Melatonin, an endogenous neurohormone secreted by the pineal gland, has a variety of physiological functions and neuroprotective effects. However, its protective role on the neural tube defects (NTDs) was not very clear. The aim of this study was to investigate the effects of melatonin on the incidence of NTDs (includ...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12282
更新日期：2015-11-01 00:00:00
abstract：:Atherosclerosis (AS) is an inflammatory disease linked to endothelial dysfunction. Melatonin is reported to possess substantial anti-inflammatory properties, which has proven to be effective in AS. Emerging literature suggests that pyroptosis plays a critical role during AS progression. However, whether pyroptosis con...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12449
更新日期：2018-03-01 00:00:00
abstract：:Melatonin and somatostatin are known to exert similar effects on locomotor activity. We have previously demonstrated that acute melatonin treatment regulates somatostatin receptor function in the rat frontoparietal cortex. However, the effects of subchronic and chronic melatonin treatment on the somatostatin receptor-...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1034/j.1600-079x.2002.02906.x
更新日期：2002-11-01 00:00:00
abstract：:Lethal oxidative stress was investigated in the dinoflagellate Gonyaulax polyedra by measuring the dying-peak of bioluminescence during circadian phases of low physiological light emission, low bioluminescence capacity, and low sensitivity to stimulatory agents. Measurements were carried out in constant darkness after...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1997.tb00353.x
更新日期：1997-11-01 00:00:00
abstract：:We tested whether combined melatonin (Mel) and exendin-4 (Ex4) treatment can better preserve glomerular structural integrity after ischemia-reperfusion (IR) injury compared with either alone. Adult male Sprague Dawley rats (n = 50) were equally divided into sham control (SC), IR, IR-Ex4 (10 μg/kg subcutaneously 30 min...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12273
更新日期：2015-11-01 00:00:00
abstract：:Melatonin secretion is an endogenous synchronizer, and it may possess some anti-aging properties. Thus we examined melatonin levels in physiological aging, in extreme senescence and in senile dementia. In healthy old (age 66-94 yr) and young subjects (age 23-39 yr) and in demented patients (age 68-91 yr) plasma melato...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2004.00125.x
更新日期：2004-05-01 00:00:00
abstract：:By use of Phaseolus vulgaris-leucoagglutinin as an anterograde in vivo tracer, a direct projection from the dorsal raphe nucleus to the pineal complex of the rat was demonstrated. The nerve fibers extended from the dorsal raphe nucleus rostrally through the mesencephalic periaqueductal grey and entered the deep pineal...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1998.tb00381.x
更新日期：1998-08-01 00:00:00
abstract：:Dim light exposure of the mother during pregnancy has been proposed as one of the environmental factors that affect the fetal brain development in schizophrenia. Melatonin circulating levels are regulated by the environmental light/dark cycle. This hormone stimulates neuronal differentiation in the adult brain. Howeve...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12421
更新日期：2017-10-01 00:00:00
abstract：:Chronic hyperhomocysteinemia is a risk factor in cardiovascular diseases and neurodegeneration. Among the putative mechanisms of homocysteine-induced neurotoxicity, an increased production of reactive oxygen species has been suggested. However, elevated homocysteine levels might disturb neurogenesis during brain devel...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2007.00506.x
更新日期：2008-03-01 00:00:00
abstract：:This study aimed to elucidate whether melatonin would exert beneficial effects on the neuronal functions of the nodose ganglion (NG) following acute hypoxic insult. The cytochrome oxidase (COX) and the nicotinamine adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry along with the nitric oxide synthase ...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2005.00238.x
更新日期：2005-09-01 00:00:00
abstract：:Environmental pollution in the form of particulate matter <2.5 μm (PM2.5 ) is a major risk factor for diseases such as lung cancer, chronic respiratory infections, and major cardiovascular diseases. Our goal was to show that PM2.5 eliciting a proinflammatory response activates the immune-pineal axis, reducing the pine...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12636
更新日期：2020-04-01 00:00:00
abstract：:The European hedgehog (Erinaceus europaeus L.) is a hibernating mammal and seasonal breeder in which numerous circadian and circannual rhythms are entrained and synchronized by photoperiod. The present study was undertaken in order to establish the involvement of the pineal gland in transducing the photoperiodic messa...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1991.tb00462.x
更新日期：1991-09-01 00:00:00
abstract：:In the present paper, binding of melatonin to purified cell nuclei from harderian glands of male and female hamsters was assessed. Binding of 125I-melatonin to cell nuclei fulfills the criteria for binding to a receptor site. Binding kinetics exhibit properties such as dependence on time and temperature as well as rev...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1034/j.1600-079x.2003.00029.x
更新日期：2003-04-01 00:00:00
abstract：:Melatonin, a neurohormone that binds to two G protein-coupled receptors MT1 and MT2, is involved in pain regulation, but the distinct role of each receptor has yet to be defined. We characterized the nociceptive responses of mice with genetic inactivation of melatonin MT1 (MT1-/- ), or MT2 (MT2-/- ), or both MT1 /MT2 ...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12671
更新日期：2020-10-01 00:00:00
abstract：:There is substantial evidence that magnetic field (MF) exposure influences melatonin secretion in animals. However, data on its influence on human melatonin levels are scarce, and seemingly contradictory. Because of its many beneficial effects, very low-frequency MF exposure is used in physiotherapy of some neurologic...
journal_title：Journal of pineal research
pub_type： 临床试验,杂志文章
doi：10.1034/j.1600-079x.2000.290203.x
更新日期：2000-09-01 00:00:00
abstract：:N-nitrosomelatonin (NOMela) is well-known for its capabilities of transnitrosating nucleophiles such as thiols and ascorbate, thereby generating nitric oxide (NO)-releasing compounds. It is unknown, however, whether NOMela can be successfully applied as a precursor of NO in a complex biological environment like a cell...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2008.00622.x
更新日期：2008-11-01 00:00:00
abstract：:Elevated levels of soluble cellular adhesion molecules have been reported in patients with acute coronary syndromes. Likewise, a relation between decreased nocturnal melatonin levels and coronary artery disease has been suggested. The aim of the present study was to investigate the day-night variations in the concentr...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2007.00529.x
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to compare the circadian control of melatonin production in teleosts. To do so, the effects of ophthalmectomy on circulating melatonin rhythms were studied along with ex vivo pineal culture in six different teleosts. Results strongly suggested that the circadian control of melatonin productio...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2007.00480.x
更新日期：2007-11-01 00:00:00
abstract：:Melatonin is an indoleamine synthesized in the pineal gland, and after its release into the blood, it has an extensive repertoire of biological activities, including antitumoral properties. In this study, we found that melatonin reduced the growth of the human melanoma cells SK-MEL-1. The antiproliferative effect was ...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2010.00765.x
更新日期：2010-08-01 00:00:00
abstract：:Seasonal day length has been linked to the prevalence of mood disorders, and however, the mechanisms underlying this relationship remain unknown. Previous work in our laboratory has shown that developmental exposure to seasonal photoperiods has enduring effects on the activity of mouse dorsal raphe serotonergic neuron...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12705
更新日期：2020-11-19 00:00:00
abstract：:The role played by postsynaptic alpha-adrenergic receptors in the stimulation of pineal melatonin production was investigated in the Syrian hamster. The studies were conducted using organ cultured pineal glands collected from both anatomically intact and superior cervical ganglionectomized hamsters. Results obtained i...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1989.tb00437.x
更新日期：1989-01-01 00:00:00
abstract：:Melatonin has shown particular promise as a neuroprotective agent to prevent motoneuron death in animal models of both amyotrophic lateral sclerosis (ALS) and spinal cord injuries (SCI). However, an understanding of the roles of endogenous melatonin receptors including MT1, MT2, and orphan G-protein receptor 50 (GPR50...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2012.01022.x
更新日期：2013-01-01 00:00:00
abstract：:Melatonin plays an important role in regulating circadian rhythms. It also acts as a potent antioxidant and regulates glucose and lipid metabolism, although the exact action mechanism is not clear. The α2-HS-glycoprotein gene (AHSG) and its protein, fetuin-A (FETUA), are one of the hepatokines and are known to be asso...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12493
更新日期：2018-09-01 00:00:00
abstract：:The effects of melatonin (3 mg/kg i.p.) and the melatonin receptor agonist S-20098 (3 mg/kg i.p.) on the vigilance states, electroencephalogram power spectra (0.25-25.0 Hz), and cortical temperature were determined in eight rats in the first 6-hr interval of the 12-hr light period. Compared to the vehicle injection bo...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079x.1994.tb00078.x
更新日期：1994-01-01 00:00:00
abstract：:Melatonin influences plant innate immunity through the mitogen-activated protein kinase (MAPK) pathway. However, the most upstream MAPK component in melatonin signaling and the dependence of generation of a reactive oxygen species (ROS) burst on melatonin synthesis and signaling remain unclear. In this study, treatmen...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12379
更新日期：2017-03-01 00:00:00
abstract：:The circadian clock plays a vital role in physiology and behavior such as the sleep-wake cycle and blood pressure and hormone levels. Immune responses also display circadian rhythmicity and particularly pineal melatonin contributes to immunological processes. Little attention, however, is given to mechanisms underlyin...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/jpi.12230
更新日期：2015-05-01 00:00:00
abstract：:Epigenetic alterations have emerged as an important mechanism involved in tumorigenesis. The epigenetic impact of DNA methylation in various types of human cancer is not completely understood. Previously, we observed melatonin-induced differential expression of miRNA and miRNA-related genes in human breast cancer cell...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2012.01027.x
更新日期：2013-01-01 00:00:00
abstract：:The association of seasonal changes in health and disease has been known for centuries. The prevalence of psychopathological symptoms with seasonal fluctuations and the use of melatonin as a biological marker of circadian and circannual rhythms is well documented. The aim of this work was to study the variability of m...
journal_title：Journal of pineal research
pub_type： 杂志文章
doi：10.1111/j.1600-079X.2006.00365.x
更新日期：2006-10-01 00:00:00
