Viability of plant cell suspensions exposed to homogeneous ultrasonic fields of different energy density and wave type.

Abstract:

:Exposure of Petunia hybrida cell suspensions to ultrasound at a frequency of 2.43 MHz in a standing wave field at an energy density of 70 Jm-3 (pressure amplitude of 0.78 MPa) decreased their mean viability to 35% after 20 min of sonication. A comparison of propagating wave and standing wave treatments at equal frequency (2.15 MHz) and energy density (8.5 Jm-3) showed, in the first case, a rapid decline in mean viability of cells (to 30% after 10 min of sonication) and, in the second case, a retaining of the initial viability (95%), respectively. Cells sonicated 4 days after subculture were more sensitive than cells sonicated 2 or 6 days after transfer to new culture medium. It was concluded that cellular viability depends primarily on the acoustic energy density, the exposure time, and the mechanical properties of the cells determined by age. As a consequence of the trapping of cells in the anti-node planes of the standing wave, propagating wave fields reduced cellular viability compared with standing wave fields at equal energy density.

journal_name

Ultrasonics

journal_title

Ultrasonics

authors

Böhm H,Anthony P,Davey MR,Briarty LG,Power JB,Lowe KC,Benes E,Gröschl M

doi

10.1016/s0041-624x(99)00166-3

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

629-32

issue

1-8

eissn

0041-624X

issn

1874-9968

pii

S0041-624X(99)00166-3

journal_volume

38

pub_type

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