Abstract：
:The aim of this study was to follow the changes of estrogen treatment on osteoblastic MBA-15 cells derived from marrow stromal origin. Following exposure to estrogen, the cells' patterns of protein synthesis and expression were monitored. The proteins synthesized by MBA-15 cells were identified in cell lysate fractionated to soluble proteins (SOL), cytoskeleton (CK), membrane and nuclei, and intermediate filaments (PL) fractions. These cellular fractions of the osteoblastic MBA-15 cell cultures were assayed on SDS-PAGE of total proteins or following radiolabeling of cells by [35S]-Methionine. Changes in cytoskeletal and membrane proteins of the control and treated cells were monitored by these assays. Reduction in expression of tubulin (TUB) and thropomyosin (TM) were observed by western blot analysis and of actin by fluorescein staining. A reduction in expression of an antigen highly expressed by osteogenic cells and detected by MoAb 85/12 was also observed in these cells. These experiments showed reduction in cytoskeletal and other cellular proteins in the stromal osteoblastic MBA-15 cells treated with 17beta-Estradiol in comparison to untreated cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Benayahu Ddoi
10.1006/bbrc.1997.6401subject
Has Abstractpub_date
1997-04-07 00:00:00pages
30-5issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(97)96401-9journal_volume
233pub_type
杂志文章abstract：:To investigate the maturation of neonatal erythrocytes and kinetics of globin expression, we induced CD34(+) cells purified from human umbilical cord blood to erythroid differentiation in different suspension culture systems containing human cord serum (CHS) and adult serum (AHS), respectively. The zeta- to alpha-glob...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.08.040
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abstract：:Variations in the UDP-glucuronosyltransferase (UGT) 1A7 gene have been found to be related to the development of hepatocellular carcinoma (HCC). Since the pathogenesis of liver cirrhosis is not dissimilar to that of HCC, we hypothesized that UGT1A7 genetic polymorphisms may be associated with liver cirrhosis. PCR-rest...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2007.11.125
更新日期：2008-02-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1996.0859
更新日期：1996-06-05 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1996.1181
更新日期：1996-08-14 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2011.06.076
更新日期：2011-07-15 00:00:00
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2011.06.163
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.2384
更新日期：1993-11-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(88)90232-x
更新日期：1988-08-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：1998-04-17 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2009.01.119
更新日期：2009-03-13 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1994.1967
更新日期：1994-07-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.03.008
更新日期：2006-05-05 00:00:00
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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doi：10.1016/j.bbrc.2007.05.211
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2011-06-17 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2004-02-27 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2004-05-21 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1999.0317
更新日期：1999-03-16 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(89)91695-1
更新日期：1989-10-16 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/s0006-291x(88)80373-5
更新日期：1988-03-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1996.5903
更新日期：1997-01-03 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(85)90646-1
更新日期：1985-01-16 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.2000.3846
更新日期：2000-12-09 00:00:00
