Abstract：
:We previously demonstrated that tissue-type plasminogen activator (t-PA) specifically bound to its receptor (t-PAR) on human umbilical vein endothelial cells (HUVEC). In addition to analyses of t-PA binding to plasminogen activator inhibitor-1 (PAI-1) in the extracellular matrix (ECM) and to the t-PAR, we further evaluated the binding of three t-PA mutants, deltaFE1X t-PA lacking finger (F), epidermal growth factor-like (E) domains and one sugar chain at Asn177 thus comprising two kringles (K1 and K2) and protease (P) domains, deltaFE3X t-PA with three glycosylation sites deleted at Asn117, 184, and 448, and deltaFEK1 t-PA comprising K2 and P domains without glycosylation. Wild-type t-PA bound to ECM with high affinity, which was completely blocked by anti-PAI-1 IgG. Wild-type t-PA, deltaFE1X t-PA and deltaFEK1 t-PA bound to two classes of binding sites with high and low affinities on monolayer HUVEC. However, all t-PAs bound to a single class of binding site in the presence of anti-PAI-1 IgG. DeltaFEK1 t-PA bound t-PAR maximally among these t-PAs. These results suggested that the high affinity binding of t-PA mainly occurred with PAI-1 on ECM while the low affinity binding was with t-PAR. The deletion of F, E domains and sugar chains had no effect on binding with t-PAR. However, since only K1-missing t-PA (deltaFEK1) exhibited significantly increased binding sites among these t-PAs, it was suggested that the binding to t-PAR was mediated mainly by K2 domain and that the increase of binding was due to direct exposure of K2 domain.
journal_name
Life Scijournal_title
Life sciencesauthors
Fukao H,Ueshima S,Okada K,Matsuo Odoi
10.1016/s0024-3205(00)80007-7subject
Has Abstractpub_date
2000-01-01 00:00:00pages
2473-87issue
25eissn
0024-3205issn
1879-0631pii
S0024320500005786journal_volume
66pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) inhibitors have been shown to treat type 2 diabetes (T2D). Since gossypol is an 11β-HSD1 inhibitor, the objective of the present study was to treat T2D and T2D-related liver fibrosis in rat model using low-dose gossypol. T2D was induced by feeding with high fat diet plus i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.02.044
更新日期：2016-03-15 00:00:00
abstract：:In some cell systems muscarinic receptor stimulation can induce proliferation or transformation. This phenomenon is subtype-specific (only m1 and m3 receptors are effective) and cell type dependent. In 1321N1 astrocytoma cells activation of m3 receptors stimulates phospholipase C, but does not induce DNA synthesis. In...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:Myelin basic protein and phosphatidylinositol-4-phosphate are phosphorylated in vitro by ATP and solubilized rat brain myelin. When both substrates are present together, the rate of phosphorylation of each is increased about eight-fold. It appears likely that the phosphate turnover of myelin basic protein and of phosp...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90597-6
更新日期：1984-01-16 00:00:00
abstract：AIMS:Pulmonary hypertension (PH) is a proliferative disorder characterized by enhanced proliferation and suppressed apoptosis of intrapulmonary vascular smooth muscle cells. Recently, network-based bioinformatics have identified let-7 family, a tumor suppressive microRNA, regulate multiple interacting targets relevant ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.11.020
更新日期：2017-02-01 00:00:00
abstract：:The analgesic buprenorphine hydrochloride (Bph) induced apoptosis-like cell death in the caspase-3-deficient human breast cancer cell line, MCF-7. This apoptosis-like cell death activated key molecules in the mitochondrial apoptotic pathway: cytochrome c, caspase-9, caspase-7, and caspase-6. Bph caused the release of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.11.032
更新日期：2004-06-04 00:00:00
abstract：:Cysteinyl-leukotrienes, i.e. leukotriene (LT) C4, D4 and E4, are inflammatory mediators and potent airway- and vasoconstrictors. Two different cysteinyl-leukotriene receptors, CysLT1 and CysLT2, have been cloned and functionally characterised using potent CysLT1 receptor antagonists and the dual CysLT1/CysLT2 receptor...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(02)01733-2
更新日期：2002-06-28 00:00:00
abstract：:Acetylcholine (ACh) may be an ovarian signaling molecule, since ACh is produced by non-neuronal granulosa cells (GCs) derived from the antral follicle, and likely also by their in vivo counterparts in the growing follicle. Furthermore, muscarinic ACh receptors (MR) are present in GC membranes and in cultured human GCs...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.01.022
更新日期：2007-05-30 00:00:00
abstract：:The biosynthesis of digitalis-like compounds (DLC) was determined in bovine and rat adrenal homogenates, as well as in primary rat adrenal cells, by following changes in the concentration of DLC using three independent sensitive bioassays: inhibition of [3H]-ouabain binding to red blood cells and competitive ouabain a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00186-6
更新日期：1998-01-01 00:00:00
abstract：:Single smooth muscle cells from rat tail artery and ventricular myocytes from neonatal rat were isolated by repeated enzyme digestion. The change in cell area as determined photographically was used as an index of cell contraction. The photographic areas of single smooth muscle cells bathed in normal Tyrode solution w...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90077-g
更新日期：1993-01-01 00:00:00
abstract：AIMS:Forkhead box (FOX) proteins constitute a huge family of transcriptional regulators, which are involved in a wide range of cancers. FOXK1 is a little studied member of FOXK subfamily. This study aimed to investigate the potential prognostic value of FOXK1 in human hepatocellular carcinoma (HCC) and explore potentia...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.068
更新日期：2019-07-01 00:00:00
abstract：:Metalloproteinases (MMP), particularly MMP-9 produced by the intratumor monocyte/macrophages, play an important role in tumor invasion and metastases. Recent clinical trials in patients with primary breast cancer suggest that bisphosphonates (BP), above all clodronate, may reduce bone metastases. The aim of the presen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00657-x
更新日期：2003-09-26 00:00:00
abstract：:Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00530-9
更新日期：1996-01-01 00:00:00
abstract：:Our previous biochemical and mechanical studies have demonstrated an increase in Ca2+ sensitivity of cardiac myofilaments in ovariectomized rats. To test whether the body weight gain associated with ovariectomy contributed some effects to the changes in myofibrillar functions, the relations of pCa (-log Ca2+ molar con...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00623-2
更新日期：2000-01-01 00:00:00
abstract：:In this paper, we describe the characterization of the expression of monoamine oxidase (MAO) in whole pancreas and in isolated islets of Langerhans from human. Classical monamine oxidase activity assays reveal that both isoforms A & B are present in human pancreas. Two complementary approaches indicated that both MAO ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00265-9
更新日期：1999-01-01 00:00:00
abstract：:We reported previously that acetylcholine (ACh)-induced endothelium-dependent relaxation of rat mesenteric microvessels depended both on nitric oxide (NO) and on a charybdotoxin (CTX)-sensitive endothelium-derived hyperpolarizing vasodilator. Cytochrome P450 (CYP)-dependent arachidonic acid metabolites act in some sys...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00413-6
更新日期：2000-02-11 00:00:00
abstract：:Effects of ethanol in vitro on membrane vesicles (microsacs prepared from mouse cerebral cortex) were evaluated by monitoring 36Cl- influx. Different assay parameters were tested to determine increased or decreased action of ethanol on GABA-activated chloride channels. The ability of 30 mM ethanol to augment 36Cl- flu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90009-g
更新日期：1990-01-01 00:00:00
abstract：:To assess the possible role of altered central serotonin function in psychiatric disorders, investigators have utilized pharmacological challenge testing with an amino acid mixture to decrease blood tryptophan concentration and, indirectly, brain serotonin levels. The aim of this pilot study was to assess the effectiv...
journal_title：Life sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0024-3205(95)00103-4
更新日期：1995-03-17 00:00:00
abstract：:We investigated the mechanism mediating cholinergic control of cell-to-cell adhesion of human epidermal keratinocytes (KC) by non-neuronal acetylcholine produced by KC themselves. We first measured cholinergic effects on the expression of desmoglein (Dsg) 1 and 3 in KC using the semi-quantitative immunofluorescence an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00087-0
更新日期：2003-03-28 00:00:00
abstract：:Since hypertension may compromise the ability to withstand hypernatremic dehydration, we investigated the impact of two experimental models of hypertension and pharmacologic normalization of blood pressure on the tolerance to chronic hypernatremic dehydration. In DOCA-salt hypertensive animals and the spontaneously-hy...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90450-6
更新日期：1990-01-01 00:00:00
abstract：:Fluorescence spectroscopy was used to examine the biophysical characteristics of human platelet membranes as a function of subject age. The structural order of membrane lipid domains was determined with the use of 1,6-diphenyl-1,3,5-hexatriene (DPH), a fluorescent probe that preferentially localizes in the hydrocarbon...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90079-7
更新日期：1985-10-14 00:00:00
abstract：:Seasonal and diurnal variations in tonic pain reactions were examined in female and male CBA/J mice maintained in a 12/12 dark/light cycle, at controlled temperature and humidity conditions. Animals were injected into the dorsum of one hindpaw with a dilute (20 microl, 1%) formalin solution. Pain-related behaviors wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02344-5
更新日期：2003-01-10 00:00:00
abstract：:The effects of social crowding in rats on nociception and analgesic actions of mu- and kappa-opioid agonists agonist qualitatively different stimuli were examined. Crowding produced higher control thresholds for electroshock and paw pinch pressure but not for noxious heat. Naloxone did not effect shock thresholds in c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90345-9
更新日期：1982-09-20 00:00:00
abstract：:Polyinosinic polycytidylic acid (poly I:C) is an analog of double stranded RNA, which is a common replication intermediate for many viruses. It acts through a toll-like receptor (TLR3) to induce a group of cytokines that can mediate host resistance to viruses and some cancers. The effect of ethanol (EtOH) on induction...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02507-9
更新日期：2003-03-07 00:00:00
abstract：:Uncontrolled recreational drugs are proliferating in number and variety. Effects of long-term use are unknown, and regulation is problematic, as efforts to control one chemical often lead to several other structural analogs. Advanced analytical instrumentation and methods are continuing to be developed to identify dru...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2012.10.031
更新日期：2013-03-19 00:00:00
abstract：:The role of p44/42 mitogen-activated protein kinase (MAPK) in the expression of intercellular adhesion molecule-1 (ICAM-1) in NCI-H292 cells, a human bronchial epithelial cell line, was analyzed. Treatment with the protein kinase C (PKC) activator 12-O-tetradecanoylphorbol 13-acetate (TPA) (16.2 nM) or interferon-gamm...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.12.016
更新日期：2004-06-11 00:00:00
abstract：:Orthodontic tooth movement induced alveolar bone resorption and formation around the teeth applied mechanical force. Although mechanical force can promote bone formation, the molecular mechanism that underlies this phenomenon is not fully understood. The purposes of this study were to determine how mechanical stress a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.10.024
更新日期：2006-05-01 00:00:00
abstract：:Bovine brain phospholipid liposomes (BC-PL) reduce plasma prolactin (PRL) levels in humans after acute administration and counteract the metoclopramide- and sulpiride-induced hyperprolactinaemia. However, BC-PL, like nomifensine, a dopamine (DA) reuptake inhibitor and therefore an indirect dopaminergic compound, does ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90395-2
更新日期：1982-07-05 00:00:00
abstract：:Premazepam (PRZ) in vitro competitively displaced 3H-diazepam (DIA), 3H-flunitrazepam (FLU) and 3H-RO 15-1788 from their binding sites on rat brain synaptosomes, with a potency intermediate to other benzodiazepines (BDZs), and Hill coefficients near 1 in different brain regions. Incubation at 37 degrees C reduced prem...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90646-5
更新日期：1984-07-23 00:00:00
abstract：AIMS:Sepsis is a major cause of morbidity and mortality in the elderly population. In prior studies, we have shown that in vivo, the inflammatory response in aged animals is exaggerated as compared to young animals and that this response likely accounts for the increased morbidity and mortality. Part of this uncontroll...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.03.009
更新日期：2010-05-08 00:00:00
abstract：AIMS:Second-generation antipsychotic drugs (SGAs) have a high risk for serious metabolic side-effects including dyslipidemia. This study aimed to investigate the acute effects of oral olanzapine treatment on the expression of genes for fatty acid and cholesterol biosynthesis in rats. MAIN METHODS:Female Sprague-Dawley...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.01.033
更新日期：2015-05-01 00:00:00
