Abstract:
BACKGROUND:Human newborns, particularly those born before full term, are more susceptible to bacterial infections as a result of impaired host defense mechanisms. Compared with adults, circulating leukocytes from human newborns (preterm and full-term gestations) and newborn rabbits (full-term gestation) have low resting levels of CD62L (L-selectin) and do not significantly increase surface expression of CD18 after inflammatory stimulation. To determine the potential utility of preterm rabbits in investigations of perinatal human conditions, the authors compared the surface expression of the beta 2-integrin CD18 and CD62L (L-selectin) on polymorphonuclear leukocytes (PMNs) from perinatal rabbits and perinatal humans, both under resting conditions and after in vitro activation with inflammatory stimulants. METHODS:After erythrocyte lysis of whole-blood samples, leukocytes from 7-day-old, full-term (31-day gestation), and preterm (24-day gestation) rabbits, as well as full-term (37-42 week gestation) and preterm (27-36 week gestation) human newborns were prepared and stimulated in vitro at 37 degrees C with either C5a or phorbol myristate acetate. After fluorescence labeling of CD18 and CD62L with monoclonal antibodies, PMN adhesion molecule expression was assessed by flow cytometry. RESULTS:Constitutive CD18 expression was not significantly different between perinatal and adult humans but was reduced in all perinatal rabbits compared with adults. Inflammatory stimulation caused significant increases in CD18 expression in adult human PMNs but not in full-term and preterm newborns. Changes in CD18 expression in adult and preterm rabbits after stimulation, although in the same direction as humans, were more variable. In both species, constitutive CD62L expression on PMNs from all perinates was significantly lower than in adults. However, CD62L was shed to similar degrees after inflammatory stimulation in all groups. CONCLUSIONS:Preterm rabbits may provide a potentially useful experimental model to study PMN adhesion and host defense in the perinatal period, particularly preterm gestations. Specific advantages and limitations of rabbits in such studies are discussed.
journal_name
Anesthesiologyjournal_title
Anesthesiologyauthors
Ramamoorthy C,Kovarik WD,Winn RK,Harlan JM,Sharar SRdoi
10.1097/00000542-199702000-00017subject
Has Abstractpub_date
1997-02-01 00:00:00pages
420-7issue
2eissn
0003-3022issn
1528-1175journal_volume
86pub_type
杂志文章相关文献
ANESTHESIOLOGY文献大全abstract::The influence of isoflurane on membrane currents, action potentials, and contraction was investigated in single cells isolated from guinea-pig ventricle. Isoflurane (1.65-4.45%) reduced the action potential duration at 20% and 90% repolarization times. When step depolarizations were applied under voltage-clamp conditi...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198811000-00017
更新日期:1988-11-01 00:00:00
abstract:BACKGROUND:It has been suggested that blood transfusion has an adverse effect on long-term health, mainly through immune modulation and tumor promotion. To further assess this concern, the authors have performed a prospective observational study with the hypothesis that after taking perioperative risk factors relevant ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3181ab6743
更新日期:2009-08-01 00:00:00
abstract::Chloroprocaine, 0.5 per cent, with physiologic saline solution, has a specific gravity of 1.007 and a osmolarity of 283 mOsm/l, which increase to a specific gravity of 1.025 and an osmolarity of 542 mOsm/l when chloroprocaine is prepared in dextrose, 5 per cent. Chloroprocaine, 2.7 per cent, shows similar increases in...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198005000-00014
更新日期:1980-05-01 00:00:00
abstract:BACKGROUND:It has been known that skeletal muscles show atrophic changes after prolonged sedation or general anesthesia. Whether these effects are due to anesthesia itself or disuse during anesthesia has not been fully clarified. Autophagy dysregulation has been implicated in muscle-wasting conditions. This study teste...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000000561
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:In airway smooth muscle (ASM), volatile anesthetics deplete sarcoplasmic reticulum (SR) Ca(2+) stores by increasing Ca(2+) "leak." Accordingly, SR replenishment becomes dependent on Ca(2+) influx. Depletion of SR Ca(2+) stores triggers Ca(2+) influx via specific plasma membrane channels, store-operated Ca(2+...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200408000-00018
更新日期:2004-08-01 00:00:00
abstract::The purpose of this study was to evaluate the analgesic contribution of intravenously administered lidocaine and to correlate it with blood levels of the drug. In a double-blind manner, 14 healthy male volunteers received saline solution or lidocaine, 0.2 per cent, at three increasingly greater rates of infusion on tw...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198001000-00005
更新日期:1980-01-01 00:00:00
abstract:BACKGROUND:Results from previous studies have shown favorable effects from the addition of buprenorphine to local anesthetics used for interscalene or axillary perivascular brachial plexus blocks. The main objective of the current study was to determine whether addition of buprenorphine could enhance bupivacaine analge...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/ALN.0b013e3181f90ce8
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Insufficient data exist on the production of compound A during closed-system sevoflurane administration with newer carbon dioxide absorbents. METHODS:A modified PhysioFlex apparatus (Dräger, Lübeck, Germany) was connected to an artificial test lung (inflow at the top of the bellow approximately/= 160 ml/min...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200109000-00030
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND:Pulsus alternans is a classic type of abnormal pulse. It can be defined as a regular alternation of pulse amplitude in which runs of weak and strong beats follow each other alternatively without any change in cycle length. It may be a sign of severe decompensated congestive heart failure. The authors infrequ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200007000-00017
更新日期:2000-07-01 00:00:00
abstract::I653 is a new volatile anesthetic structurally similar to enflurane and isoflurane. Since enflurane can induce convulsions, whereas isoflurane progressively depresses cortical electrical activity, the authors believed it important to assess the effect of I653 on the EEG (in both the "time" and "frequency" domain). The...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198809000-00002
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND:Chronic neck pain after whiplash injury is caused by cervical zygapophysial joints in 50% of patients. Diagnostic blocks of nerves supplying the joints are performed using fluoroscopy. The authors' hypothesis was that the third occipital nerve can be visualized and blocked with use of an ultrasound-guided te...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/00000542-200602000-00016
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND:Ca is the dominant second messenger in primary sensory neurons. In addition, disrupted Ca signaling is a prominent feature in pain models involving peripheral nerve injury. Standard cytoplasmic Ca recording techniques use high K or field stimulation and dissociated neurons. To compare findings in intact dors...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3181e0ef3f
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Dexmedetomidine is a sedative with modest analgesic efficacy, whereas remifentanil is an opioid analgesic with modest sedative potency. Synergy is often observed when sedative-hypnotics are combined with opioid analgesics in anesthetic practice. A three-phase crossover trial was conducted to study the pharma...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/ALN.0000000000002882
更新日期:2019-11-01 00:00:00
abstract::Changes in hepatic plasma flow (HPF) during sodium nitroprusside (SNP) induced hypotension were studied in 14 patients undergoing intracranial aneurysm surgery under neurolept anesthesia. Patients were monitored with the use of a radial artery catheter and a thermistor-tipped Swan-Ganz catheter. Hypotension was induce...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198509000-00008
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND:Massive release of central excitatory neurotransmitters is an important initial step in ischemic neuronal injury, and modification of this process may provide neuroprotection. We studied the protective effects of the voltage-dependent sodium channel antagonist riluzole and the N-methyl-d-aspartate receptor a...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200011000-00025
更新日期:2000-11-01 00:00:00
abstract::This study was performed to determine the potency of succinylcholine using the single-dose technique, and to test the ability of the cumulative dose technique for generating dose-response data. Thirty-eight adult patients received single doses (n = 18), cumulative doses (n = 10), or cumulative doses of succinylcholine...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND:Cisatracurium, one of the ten isomers in atracurium, is a nondepolarizing muscle relaxant with an intermediate duration of action. It is more potent and less likely to release histamine than atracurium. As one of the isomers composing atracurium, it presumably undergoes Hofmann elimination. This study was co...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/00000542-199602000-00007
更新日期:1996-02-01 00:00:00
abstract::The effect of halothane, at clinically relevant concentrations on the ability of human polymorphonuclear leukocytes (PMNL) to kill the most frequently isolated gram-negative organisms responsible for human bacteremias, Escherichia coli and Klebsiella pneumoniae, was studied. Exposure of PMNL to 0.75 per cent halothane...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198155060-00009
更新日期:1981-12-01 00:00:00
abstract::The tape recorded EEGs of 127 patients anesthetized with large doses of opioids were retrospectively analyzed for evidence of opioid-induced seizures, and in particular, correlated with movements that occurred during induction and could be clinically interpreted as seizures. Bilateral EEG leads in patients receiving f...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198912000-00008
更新日期:1989-12-01 00:00:00
abstract:BACKGROUND:Cortical acetylcholine originates in the basal forebrain and is essential for maintaining normal cognition and arousal. Morphine impairs these cholinergically mediated cortical functions. The current study tested the hypothesis that morphine decreases prefrontal cortical acetylcholine release by acting at th...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200510000-00016
更新日期:2005-10-01 00:00:00
abstract::No significant change in pseudocholinesterase levels was observed in random specimens of whole blood stored for as long as 30 days. Levels in plasma anticoagulated with heparin or ethylenediaminetetraacetic acid declined only slightly. Therefore, massive transfusions do not contraindicate succinylcholine administratio...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-197605000-00014
更新日期:1976-05-01 00:00:00
abstract:BACKGROUND:Hypoperfusion of the intestinal mucosa remains an important clinical problem during sepsis. Impairment of the autoregulation of microcirculatory blood flow in the intestinal tract has been suggested to play an important role in the development of multiple organ failure during sepsis and surgery. The authors ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200303000-00014
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Mechanical ventilation (MV) is a life-saving intervention in patients with acute respiratory failure. However, prolonged MV results in ventilator-induced diaphragm dysfunction (VIDD), a condition characterized by both diaphragm fiber atrophy and contractile dysfunction. Previous work has shown that calpain, ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000000245
更新日期:2014-07-01 00:00:00
abstract::Clinical studies have reported that body core temperature decreases during prolonged surgery and anesthesia. Although this finding has been attributed primarily to increased heat loss resulting from exposure of body cavities and infusion of cold solutions, it is generally recognized that anesthesia interferes with the...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199204000-00011
更新日期:1992-04-01 00:00:00
abstract::The effects of continuous positive airway pressure (CPAP) on cardiovascular dynamics and pulmonary shunt (QS/QT) were investigated in 12 dogs before and during sodium nitroprusside infusion that decreased mean arterial blood pressure 40-50 per cent. Before nitroprusside infusion, 5 cm H2O CPAP significantly, P less th...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-197705000-00008
更新日期:1977-05-01 00:00:00
abstract:BACKGROUND:(human ether-a-gogo related gene) encodes the cardiac rapidly activating delayed rectifier potassium currents (I(kr)), which play an important role in cardiac action potential repolarization. General anesthetics, like halothane, can prolong Q-T interval, suggesting that they act on myocellular repolarization...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200210000-00026
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Chronic postsurgical pain, a significant public health problem, occurs in 10 to 50% of patients undergoing major surgery. Acute pain induces endogenous analgesia termed conditioned pain modulation (CPM), and the strength of CPM preoperatively predicts the likelihood of chronic postsurgical pain. The relation...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000000593
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:In the partial CO(2) rebreathing method, monitored changes in CO(2) elimination and end-tidal CO(2) in response to a brief rebreathing period are used to estimate cardiac output. However, dynamic changes in CO(2) production during ischemia and reperfusion may affect the accuracy of these estimates. This stud...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/00000542-200308000-00009
更新日期:2003-08-01 00:00:00
abstract:BACKGROUND:The effects of thoracic epidural anesthesia (TEA) on myocardial repolarization and arrhythmogenicity are only incompletely understood. This is primarily because of the lack of appropriate experimental models. In most of the studies performed thus far, TEA was used in anesthetized animals. Baseline anesthesia...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200107000-00023
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:The probability of recovering consciousness in acute brain-injured patients depends on central nervous system damage and complications acquired during their stay in the intensive care unit. The objective of this study was to establish a relation between the Bispectral Index (BIS) and other variables derived ...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/00000542-200407000-00009
更新日期:2004-07-01 00:00:00