Abstract:
BACKGROUND:Ca is the dominant second messenger in primary sensory neurons. In addition, disrupted Ca signaling is a prominent feature in pain models involving peripheral nerve injury. Standard cytoplasmic Ca recording techniques use high K or field stimulation and dissociated neurons. To compare findings in intact dorsal root ganglia, we used a method of simultaneous electrophysiologic and microfluorimetric recording. METHODS:Dissociated neurons were loaded by bath-applied Fura-2-AM and subjected to field stimulation. Alternatively, we adapted a technique in which neuronal somata of intact ganglia were loaded with Fura-2 through an intracellular microelectrode that provided simultaneous membrane potential recording during activation by action potentials (APs) conducted from attached dorsal roots. RESULTS:Field stimulation at levels necessary to activate neurons generated bath pH changes through electrolysis and failed to predictably drive neurons with AP trains. In the intact ganglion technique, single APs produced measurable Ca transients that were fourfold larger in presumed nociceptive C-type neurons than in nonnociceptive Abeta-type neurons. Unitary Ca transients summated during AP trains, forming transients with amplitudes that were highly dependent on stimulation frequency. Each neuron was tuned to a preferred frequency at which transient amplitude was maximal. Transients predominantly exhibited monoexponential recovery and had sustained plateaus during recovery only with trains of more than 100 APs. Nerve injury decreased Ca transients in C-type neurons, but increased transients in Abeta-type neurons. CONCLUSIONS:Refined observation of Ca signaling is possible through natural activation by conducted APs in undissociated sensory neurons and reveals features distinct to neuronal types and injury state.
journal_name
Anesthesiologyjournal_title
Anesthesiologyauthors
Gemes G,Rigaud M,Koopmeiners AS,Poroli MJ,Zoga V,Hogan QHdoi
10.1097/ALN.0b013e3181e0ef3fsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
134-46issue
1eissn
0003-3022issn
1528-1175journal_volume
113pub_type
杂志文章相关文献
ANESTHESIOLOGY文献大全abstract::To determine whether anesthesiologists can manually detect significant changes in pulmonary compliance in neonates using an "educated hand," the authors tested whether clinicians could detect clamping of an endotracheal tube connecting a neonatal lung model to one of three anesthesia breathing circuits. The test lungs...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199110000-00022
更新日期:1991-10-01 00:00:00
abstract::The anesthetic effects and interactions of midazolam and fentanyl were determined in terms of their reduction of enflurane MAC in dogs, and the effects of their specific antagonists were also investigated. Control enflurane MAC was determined by the tail clamp method in 18 mongrel dogs. Each animal then received an iv...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198904000-00021
更新日期:1989-04-01 00:00:00
abstract:BACKGROUND:Recent interest in shortening the fasting interval after ingestion of milk products demonstrated large volumes of breast milk in the stomach 2 h after breastfeeding. Although aspiration is a rare event, if it were to occur with human breast milk, it is important to understand the extent of the lung injury th...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199606000-00015
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:Knowledge of normal front teeth-to-carina distance (FT-C) might prevent accidental bronchial intubation. The aim of the current study was to measure FT-C and to examine whether the Morgan formula for oral intubation depth, i.e., endotracheal tube (ETT) position at front teeth (cm) = 0.10 x height (cm) + 5, g...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3181730288
更新日期:2008-06-01 00:00:00
abstract::Initial studies have suggested that oral transmucosal fentanyl citrate (OTFC) in a dose of 15-20 micrograms/kg may be a safe and effective preanesthetic medication in children and adults, but this has not been demonstrated in a randomized, double-blind fashion. The purpose of this study was to determine in a randomize...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Propofol has been implicated as causing intraoperative bradyarrhythmias. Furthermore, the effects of propofol on the electrophysiologic properties of the sinoatrial (SA) node and on normal atrioventricular (AV) and accessory pathways in patients with Wolff-Parkinson-White syndrome are unknown. Therefore, thi...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/00000542-199504000-00011
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:The authors recently established that the analgesic actions of the inhalation anesthetic nitrous oxide were mediated by noradrenergic bulbospinal neurons and spinal alpha2B adrenoceptors. They now determined whether noradrenergic brainstem nuclei and descending spinal pathways are responsible for the antinoc...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200202000-00023
更新日期:2002-02-01 00:00:00
abstract::Opioid receptors have been targeted for the treatment of pain and related disorders for thousands of years and remain the most widely used analgesics in the clinic. Mu (μ), kappa (κ), and delta (δ) opioid receptors represent the originally classified receptor subtypes, with opioid receptor like-1 (ORL1) being the leas...
journal_title:Anesthesiology
pub_type: 杂志文章,评审
doi:10.1097/ALN.0b013e318238bba6
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:The degree to which intrathecally administered morphine can alleviate hypersensitivity in animals after peripheral nerve injury is controversial, and the mechanisms by which morphine works in these circumstances are uncertain. In normal animals, morphine induces adenosine release, and in vitro data suggest t...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200502000-00027
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:The potential benefit of propofol dose regimens that use physiologic pharmacokinetic modeling to target the brain has been demonstrated in animals, but no data are available on the rate of propofol distribution to the brain in humans. This study measured the brain uptake of propofol in humans and the simulta...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章
doi:10.1097/00000542-200212000-00006
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:The spinal delivery of the cholinesterase inhibitor neostigmine yields analgesia in rats and augments the analgesic effects of alpha 2 agonists in sheep. To assess its activity in humans, preclinical toxicology studies to define its safety were required in two species. METHODS:Rats with chronic intrathecal ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199502000-00012
更新日期:1995-02-01 00:00:00
abstract:BACKGROUND:The role of endothelial nitric oxide synthase (eNOS) in isoflurane postconditioning (IsoPC)-elicited cardioprotection is poorly understood. The authors addressed this issue using eNOS mice. METHODS:In vivo or Langendorff-perfused mouse hearts underwent 30 min of ischemia followed by 2 h of reperfusion in th...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3181c4a607
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to characterize cervical cord, root, and bony spine claims in the American Society of Anesthesiologists Closed Claims database to formulate hypotheses regarding mechanisms of injury. METHODS:All general anesthesia claims (1970-2007) in the Closed Claims database were searched to id...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3182104859
更新日期:2011-04-01 00:00:00
abstract::Intraventricular conduction disorders and reentrant arrhythmias in dogs can be produced by high plasma bupivacaine concentrations. The authors' aim was to determine if these conduction disturbances also occurred at moderate plasma bupivacaine concentrations (2.2-3.7 micrograms/ml) when in association with other factor...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199006000-00016
更新日期:1990-06-01 00:00:00
abstract:BACKGROUND:There is little information concerning the carbon dioxide-related effects on respiratory mechanics in anesthetized, paralyzed subjects; however, hypocapnia or hypercapnia is often permitted in patients with severe brain injury or acute respiratory distress syndrome. Therefore, the carbon dioxide dependence o...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200104000-00013
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:Adaptive support ventilation (ASV) allows the clinician to set a maximum plateau pressure (PP) and automatically adjusts tidal volume to keep PP below the set maximum. METHODS:ASV was compared to a fixed tidal volume of 6 ml/kg. ASV determined the respiratory rate and tidal volume based on its algorithms. M...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3181b55f8f
更新日期:2009-10-01 00:00:00
abstract::The inhibitory effects of three inhalation anesthetics, i.e., halothane, enflurane, and isoflurane, on superoxide production and the intracellular mobilization of calcium in human neutrophils were studied. The superoxide production induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP) was inhibited by the anesthet...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198601000-00002
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:Mortality after noncardiac surgery has been associated with the "triple low state," a combination of low mean arterial blood pressure (<75 mmHg), low bispectral index (<45), and low minimum alveolar concentration of volatile anesthesia (<0.70). The authors set out to determine whether duration of a triple lo...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000000281
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Early acquisition of critical competencies by novice anesthesiology residents is essential for patient safety, but traditional training methods may be insufficient. The purpose of this study was to determine the effectiveness of high-fidelity simulation training of novice residents in the initial management ...
journal_title:Anesthesiology
pub_type: 杂志文章,随机对照试验
doi:10.1097/ALN.0b013e3181c62d43
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:In cardiac myocytes, lidocaine reduces but mexiletine increases adenosine triphosphate (ATP)-sensitive K+ currents, suggesting that these class Ib antiarrhythmic drugs may differentially modify the activity of ATP-sensitive K+ channels. The effects of lidocaine and mexiletine on arterial relaxations induced ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199904000-00032
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Anesthetics are believed to alter functional connectivity across brain regions. However, network-level analyses of anesthesia, particularly in humans, are sparse. The authors hypothesized that propofol-induced loss of consciousness results in functional disconnection of human sensorimotor cortices underlying...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000001940
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:Acute respiratory distress syndrome (ARDS) is a devastating condition with an estimated mortality exceeding 30%. There are data suggesting risk factors for ARDS development in high-risk populations, but few data are available in lower incidence populations. Using risk-matched analysis and a combination of cl...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0b013e3182794975
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Elimination of daily-routine chest radiographs (CXRs) may influence chest computed tomography (CT) and ultrasound practice in critically ill patients. METHODS:This was a retrospective cohort study including all patients admitted to a university-affiliated intensive care unit during two consecutive periods o...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/01.anes.0000296069.00566.16
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Investigators in the authors' laboratory previously established the critical participation of the cerulospinal noradrenergic pathway in muscular rigidity elicited by fentanyl. The identification of colocalization of glutamate with tyrosine hydroxylase in most locus ceruleus neurons suggests a role for cerulo...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-199712000-00024
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:The linearity of cisatracurium elimination and its concentration-effect relation were determined as part of a traditional rich data study with three dose levels in patients receiving balanced anesthesia. METHODS:Forty-eight adults with American Society of Anesthesiologists status I-II were randomized to rec...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200108000-00010
更新日期:2001-08-01 00:00:00
abstract::Deliberate hypotension was induced with isoflurane (mean inspired concentration 2.3 +/- 1.0%) in 12 patients undergoing craniotomy for clipping of cerebral aneurysms. Global cerebral blood flow (CBF) was measured before, during, and after hypotension. Arterio-venous O2 content difference was measured concomitantly, an...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-198603000-00001
更新日期:1986-03-01 00:00:00
abstract:BACKGROUND:The discrepancy between predicted effect-site concentration and measured bispectral index is problematic during intravenous anesthesia with target-controlled infusion of propofol and remifentanil. We hypothesized that bispectral index during total intravenous anesthesia would be more accurately predicted by ...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/ALN.0000000000001892
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Previous attempts at improving operating room utilization have generally emphasized more accurate scheduling, starting the first case on time, and reducing turnover time. Surgical case cancellations have largely been ignored except for recommendations for preoperative screening and good physician-patient com...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200604000-00029
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:The combining of epidural anesthesia with general anesthesia impairs central and peripheral thermoregulatory control and therefore is often accompanied by unintended intraoperative hypothermia. However, little is known about the cardiovascular response to hypothermia during combined epidural and general anes...
journal_title:Anesthesiology
pub_type: 杂志文章
doi:10.1097/00000542-200104000-00023
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:The dose-response relation for spinal ropivacaine is undetermined, and there are few data available for obstetric patients. METHODS:In a prospective, randomized, double-blind investigation, the authors studied 72 patients undergoing elective cesarean delivery. An epidural catheter was placed at the L2-L3 ve...
journal_title:Anesthesiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000542-200112000-00011
更新日期:2001-12-01 00:00:00