Abstract:
:Interferon-alpha (IFN-alpha) has been found to exert multiple enhancing effects in the immune response in vitro, IFN-alpha has been also used in clinical trials with variable response rates. The aim of the present study was to assess the effectiveness of IFN-alpha in the treatment of 25 patients with malignant pleural or peritoneal effusions caused by lung, and metastatic breast and ovarian cancer. Clinical responses were correlated with a) the ratio of malignant effusion (ME)-associated tumor cells to ME-associated mononuclear cells (MEMNC), b) MEMNC-derived cytotoxic responses against autologous or allogeneic tumor targets, and c) major histocompatibility complex (MHC) antigen expression on tumor cells. After partial drainage of pleural or peritoneal fluid, the patients were allocated to receive 10 million units of IFN-alpha by intrapleural or intraperitoneal injection at weekly intervals. The treatment was terminated if the malignant effusion disappeared or the patients had received four to six consecutive procedures. None of the patients received concomitant systemic chemotherapy or radiation therapy. MEMNC and tumor cells were isolated by centrifugation on discontinous percoll density gradients. Cytotoxic and phenotypic profiles of MEMNG were analyzed before and after treatment with IFN-alpha. An improvement was observed in patients with increased ratios of tumor cells to malignant effusion-associated mononuclear cells (MEMNC) in the effusions. In the same patients MEMNC were overpopulated by CD8+ T lymphocytes. In this group of patients the administration of IFN-alpha was associated with 25% complete response and 75% partial response rates. In contrast only 17% partial responses were achieved in patients whose effusions had decreased tumor cell to MEMNC ratios. The immunomodulation induced by IFN-alpha in vivo was also tested. Thus in a group of 6 patients, treatment with IFN-alpha resulted in the induction of CD8+ cell-mediated lysis against autologous tumor cells which was associated with PR (two patients). Natural killer (NK)-cell activity, and MHC class I antigen expression on effusion-associated tumor cells were also enhanced during treatment, but were not correlated with the outcome of the therapy since similar findings were also observed in the 4 non-responders. Local infusions of IFN-alpha provide an effective alternative treatment for malignant effusion in patients with lung, breast, and ovarian cancer. Increased ratios of tumor cells to MEMNC and the presence of CD8+ T lymphocytes within the malignant effusions may play an important role in the outcome of such a treatment with IFN-alpha but more patients need to be studied for definite conclusions.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Stathopoulos GP,Baxevanis CN,Papadopoulos NG,Zarkadis IK,Papacostas P,Michailakis E,Tsiatas ML,Papamichail Msubject
Has Abstractpub_date
1996-11-01 00:00:00pages
3855-60issue
6Beissn
0250-7005issn
1791-7530journal_volume
16pub_type
杂志文章abstract:BACKGROUND:The aim of the study was to document the delay in diagnosis of Hodgkin's disease (HD) and clarify the impact on clinical stage and survival in our region. MATERIAL AND METHODS:A retrospective study of the records of 50 patients treated for primary HD in northern-Norway in the time period 1985 - 1993 was per...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::The androgen-signaling pathway plays a critical role in prostate cancer development and progression. We have recently demonstrated that the Wilms' tumor suppressor gene product, WT1, binds to multiple sites in the androgen receptor (AR) promoter and transcriptionally represses the AR gene promoter in vitro. We asked w...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND/AIM:The aim of the present study was to determine the efficacy of trabectedin combined with FOLFIRI (irinotecan, leucovorin and 5-fluorouracil) on a colorectal cancer (CRC) patient-derived orthotopic xenograft (iPDOX) mouse model. MATERIALS AND METHODS:A CRC tumor from a patient previously established in nu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13860
更新日期:2019-12-01 00:00:00
abstract::A relationship was established between estradiol 16 alpha-hydroxylation, papillomavirus and its malignant sequelae. In this study, we determined that 16 alpha-hydroxylation was substantial in normal larynx and further increased in laryngeal papillomas. Estradiol or 16 alpha-hydroxyestrone stimulated proliferation in b...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Adenovirus(ADV) mediated thymidine kinase(TK) gene therapy followed by ganciclovir(GCV) administration is widely used in different types of cancer. ACV shares the same mechanism of selective cell killing in ADV/TK positive cells as GCV and can be used at 4.5 times higher doses in patients without significant side effe...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::Tumor-specific antigens are important in the study of tumor biology, tumor diagnosis and prognosis and possibly in tumor therapy. We demonstrate in this report that surgical specimens of colon, breast and ovarian tumors in native-state in vitro gel-supported three-dimensional in vitro histoculture maintain in vivo-lik...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract:UNLABELLED:Adenomatous polyps are precursor lesions for colorectal carcinoma. The risk of cancer development has been associated with age and size, amount of villous component and high-grade dysplasia of adenomas. The subject-related and adenoma-related risk factors for severely dysplastic lesions were further investig...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::Intratumoral (cervix uterine tumors) distribution of platinum was studied in ten patients after IV administration of CDDP. Sequential tumoral measurement of platinum was carried out 4, 24, and 48 h after the IV infusion. Platinum concentration in the tumor samples ranged from 0 to 29,5 ng/mg tissue. For all patients, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:Mitoxantrone resistance has been related to the expression of a drug efflux pump breast cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (D...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND/AIM:The Glasgow Prognostic Score (GPS), an inflammation-based prognostic score, has been shown to predict the clinical outcomes of a variety of cancer types. The aim of this study was to determine whether the GPS predicts clinical outcomes of patients with pancreatic cancer treated with adjuvant chemotherapy...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract::Paclitaxel has been approved as second-line therapy after anthracyclines in AIDS-Kaposi's sarcoma (KS) patients. To date, only one patient with classical KS and treated with standard dose (175 mg/m2) 3-weekly paclitaxel as first line therapy has been reported in the literature. Herein, the first case is presented of a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:The mitochondrial protein translocase of inner mitochondrial membrane 17 homolog A (TIMM17A) has been identified as a biomarker of breast cancer. The present study aimed to investigate the biological role of TIMM17A in human breast cancer cells. MATERIALS AND METHODS:Anti-TIMM17A transgenes were stably tran...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract::Neurological complications of both fluorouracil (5-FU) and its oral prodrug, capecitabine, have been described in the literature. This study reported the case of a 70-year-old female with metastatic adenocarcinoid of the rectum who developed hyperammonemic encephalopathy, following infusional 5-FU therapy, manifesting...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Human papillomavirus (HPV)-positive tonsillar- and base of tongue cancer is increasing epidemically and has much better outcome than corresponding HPV-negative cancer and most other head and neck cancers with around 80% 3-year disease free survival with conventional radiotherapy and surgery. Consequently, most HPV-pos...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.11958
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Screening gastroscopic examinations were performed in a cohort of individuals at high risk for developing gastric carcinoma (GC). PATIENTS AND METHODS:Five gastric biopsies were obtained following the Houston schema. Five histological parameters of gastritis were investigated: acute gastritis, chronic gastr...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract::Basal level of asparagine synthetase mRNA in BALB3T3 cells was elevated when the cells were shifted from medium containing a high concentration (3.3 mM) of asparagine to one lacking asparagine. We then studied whether the expression of asparagine synthetase mRNA is also mediated through other asparagine-independent si...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract:AIM:To clarify how patients with epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma with acquired resistance to EGFR-tyrosine kinase inhibitors (TKIs) respond to radiotherapy (RT) for brain metastases. PATIENTS AND METHODS:Forty-seven patients were divided into the following three groups: a TKI-naïve g...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::In medullary carcinoma of the thyroid (MTC), multidrug resistance (MDR) remains the major obstacle to effective chemotherapy. In this work MDR was investigated in TT cells, a human MTC cell line. We studied the effect of an efficient MDR agent (SDZ PSC 833) on doxorubicin (DOX)-induced cytotoxicity in TT cells culture...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:The antiemetic effects of serotonin receptor antagonists during chemoradiotherapy for solid tumors have never been reported. We have developed hyperthermo-chemo-radiotherapy (HCR) for esophageal cancer. However, with this treatment, the more potent the chemotherapy was, the more frequently emesis was experie...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:The influence of age on the patterns and outcomes of external beam radiotherapy for clinically localized prostate cancer patients was examined. MATERIALS AND METHODS:The Japanese Patterns of Care Study surveys were used to compare the processes and outcomes of radical external beam radiotherapy in 140 elder...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Bisphosphonates (BPs) are considered the standard of care in metastatic breast cancer (MBC) patients with bone metastases. Because the survival for these patients may be prolonged compared with those patients with visceral metastases, they may be prescribed BPs for several years. The effects of this long-ter...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:The safety and efficacy of docetaxel plus S-1 combination chemotherapy as a first-line treatment in patients with advanced or recurrent gastric cancer was verified retrospectively. PATIENTS AND METHODS:Eighteen patients with advanced or recurrent gastric cancer were enrolled. The regimen used was intravenou...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::In pancreatic neoplasias mutations in the first exon (codon 12) of K-ras gene occur at high frequency and seem to have a diagnostic significance. We set up the DGGE conditions to search for these mutations in pancreatic tumor sample DNAs. All samples were directly classified by simply comparing their DGGE patterns wit...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract:UNLABELLED:The aim of the present study was to evaluate the effectiveness and safety of hepatic re-resection for breast cancer liver metastases. PATIENTS AND METHODS:Between January 2004 and December 2014 seven patients were submitted to liver re-resection for breast cancer liver metastases at our Center. The main inc...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND/AIM:In the present article we review on the use of Volumetric Modulated Arc Therapy (VMAT) for a small lung nodule that was centrally located in close proximity to the mediastinal structures. CASE REPORT:An inoperable patient with central, clinical stage IA adenocarcinoma of the right lung was treated with ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2013-10-01 00:00:00
abstract::Ten cell sublines the MDA-MB 231 human breast cell line were analyzed for their phenotypic diversity: morphology, karyotype, growth rate, clonogenicity in semisolid medium, tumorigenicity in nude mice, number and affinity of nuclear receptors for oestradiol, progesterone and glucocorticoids. Karyotypic analysis showed...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract:UNLABELLED:The aim of this study wsa to evaluate the additive effect of valproic acid (VPA) to γδ T-cell cytotoxicity against bladder cancer cells. MATERIALS AND METHODS:Human bladder cancer cell lines TCCSUP and 253J were treated with VPA and mRNA expression of natural killer group 2D (NKG2D) ligands was determined. ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND/AIM:Long-term results of a study investigating potential prognostic factors for treatment outcomes in patients with stage III esophageal cancer are presented. PATIENTS AND METHODS:In 64 patients, the impact of tumor cell expression of erythropoietin (EPO) and erythropoietin-receptor (EPO-R) and ten addition...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:To improve long-term survival by reducing toxicity in intermediate stage Hodgkin's disease patients, we compared the effects of involved field (IF) versus extended field (EF) irradiation administered after four cycles of ABVD regimen. MATERIALS AND METHODS:Two hundred and ten Hodgkin's disease patients, at ...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:A critical step in the delivery of nanomedicines to tumour cells is transporting these particles through the extracellular matrix. Tumour-specific anticancer agents, such as encapsulated drugs, proteins, and genes, show low uptake in tumour tissue. It is not clear whether the collagen network or the glycosam...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00