Abstract:
BACKGROUND:While metabolically generated oxidants are produced locally in experimental glomerular diseases, little is still known of their significance and the respective scavenger systems in human glomerular diseases. METHODS:Here we studied kidneys from patients with congenital nephrotic syndrome of the Finnish type (CNF), a human model disease of isolated proteinuria. Expression of specific mRNAs for a major antioxidant system against lipoperoxidation [phospholipid hydroperoxide glutathione peroxidase (PHGPx)] and for mitochondrial proteins were studied in Northern blotting together with analysis of PHGPx in semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The respective proteins and lipoperoxide (LPO) adducts malonyldialdehyde (MDA) and 4-hydroxynonenal (4-HNE) were analyzed in immunohistochemistry. RESULTS:PHGPx and the mitochondrially encoded subunits of cytochrome-c-oxidase were distinctly down-regulated within the glomeruli of CNF kidneys. These changes were confirmed in semiquantitative RT-PCR. Increases of lipoperoxidation products MDA and 4-HNE were constantly found in the glomeruli of CNF. In agreement with findings in CNF, similar results were obtained in biopsies from other human glomerular diseases. CONCLUSIONS:These findings suggest that local mitochondrial damage initiates LPO, which then causes deposition of the cytotoxic LPO products in glomeruli, as seen especially in CNF kidneys. Together with down-regulation of the local antioxidant protection, these may be important pathophysiologic mechanisms in human glomerular disease.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Solin ML,Ahola H,Haltia A,Ursini F,Montine T,Roveri A,Kerjaschki D,Holthöfer Hdoi
10.1046/j.1523-1755.2001.059002481.xsubject
Has Abstractpub_date
2001-02-01 00:00:00pages
481-7issue
2eissn
0085-2538issn
1523-1755pii
S0085-2538(15)47498-8journal_volume
59pub_type
杂志文章abstract::Although much emphasis has been placed on screening for albuminuria in type II diabetic patients, less attention has been focused on the role of glomerular filtration rate (GFR) in the assessment of risk. Herein, we examined the association between GFR and vascular complications in a consecutive cohort of 5174 type II...
journal_title:Kidney international
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journal_title:Kidney international
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journal_title:Kidney international
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journal_title:Kidney international
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pub_type: 杂志文章,随机对照试验
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journal_title:Kidney international
pub_type: 杂志文章,评审
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journal_title:Kidney international
pub_type: 杂志文章
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abstract:BACKGROUND:Altered mineral metabolism contributes to bone disease, cardiovascular disease, and other clinical problems in patients with end-stage renal disease. METHODS:This study describes the recent status, significant predictors, and potential consequences of abnormal mineral metabolism in representative groups of ...
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journal_title:Kidney international
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journal_title:Kidney international
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doi:10.1038/ki.1994.56
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journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1989.306
更新日期:1989-12-01 00:00:00
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journal_title:Kidney international
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journal_title:Kidney international
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更新日期:2007-09-01 00:00:00
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journal_title:Kidney international
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journal_title:Kidney international
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abstract::Renal dysfunction is a common and serious complication in multiple myeloma (MM) patients. Renal proximal tubule injury is characteristic in MM, and may result in disturbed renal handling of various vitamins. The abnormal excretion of vitamins in urine may result in their low serum levels. The goal of this study was to...
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更新日期:2006-01-01 00:00:00