[Detection of anti-Treponema pallidum antibodies with colloidal gold immunoprobes].

Abstract:

BACKGROUND:The aim of this study was to evaluate the usefulness of colloidal gold probes widely used in histochemistry and electronic microscopy for the serologic diagnosis of syphilis. METHODS:One hundred eighty-nine sera which had been submitted to the traditional fluorescent trigenemic absorption (FTA-ABS) test and had later been tested with the new colloidal gold probe method (IAT-ABS). RESULTS:Out of a total of 103 positive samples by FTA-ABS all were positive when tested by gold immunoprobes (sensitivity 100%). Eighty-six samples which were negative by immunofluorescence were all negative by IAT-ABS (specificity 100%). CONCLUSIONS:The new method of colloidal gold probes with the current microscopy is useful for confirming serologic diagnosis of syphilis.

authors

Burgos JD,Mendoza M,Vinasco J,Hernández R

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

304-7

issue

5

eissn

0213-005X

issn

1578-1852

journal_volume

14

pub_type

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