Abstract：
INTRODUCTION:The use of the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast in some clinical specimens for the identification of colonizing microorganisms. The objective of the study was to analyze the validity values for the prediction of colonization and catheter-related bloodstream infection (C-RBSI) of the MALDI-TOF mass spectrometry performed at all intravascular catheters that arrived in the microbiology laboratory. METHODS:Catheter tips (after performing the roll-plate technique) were tested by MALDI-TOF mass spectrometry during a period of 3-months. The gold standard for colonization and C-RBSI were, respectively: the presence of ≥15cfu/plate in the catheter tip culture; and the isolation of the same microorganism(s) in blood cultures as well as in the colonized catheter (during the 7days before or after catheter withdrawal). RESULTS:A total of 182 intravascular catheters were collected. The overall colonization rate detected by roll-plate technique and MAL-TOF mass spectrometry was 31.9% and 32.4%, respectively. Overall, there were 33 (18.1%) episodes of C-RBSI. The validity values of the MALDI-TOF mass spectrometry for the identification of colonization and C-RBSI were, respectively: sensitivity (69.0%/66.7%), specificity (84.7%/75.2%), positive predictive value (65.6%/36.1%), and negative predictive value (86.8%/92.6%). Conclusion MALDI-TOF mass spectrometry could be an alternative diagnostic tool for ruling out C-RBSI. However, despite it showing to be faster than conventional culture, future studies are required in order to improve the pre-analytical process.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Guembe M,Rodríguez-Sánchez B,Ruiz A,Martín-Rabadán P,Rodríguez-Créixems M,Bouza Edoi
10.1016/j.eimc.2014.01.011subject
Has Abstractpub_date
2014-06-01 00:00:00pages
372-4issue
6eissn
0213-005Xissn
1578-1852pii
S0213-005X(14)00058-5journal_volume
32pub_type
杂志文章abstract：BACKGROUND:In recent years an increase has been observed in the prevalence of meningococcal infection by Neisseria meningitidis serogroup C and in the appearance of strains with moderate resistance to penicillin. PATIENTS AND METHODS:A microbiologic study of the cases of meningococcal infection of serogroup C treated ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVES:To analyse and compare the ability of procalcitonin (PCT), C-reactive protein (CRP) and leukocytes to differentiate true bacteraemia from contaminated blood cultures in patients seen in the emergency department (ED) for an episode of infectious disease. METHODS:Observational, retrospective and descriptive a...
journal_title：Enfermedades infecciosas y microbiologia clinica
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abstract：BACKGROUND:Helicobacter pylori infection is probably the most common chronic bacterial infection in the world. The consequences of infection are pathologies like peptic ulcer, chronic gastritis, gastric cancer and gastric MALT lymphoma. The aim of this study was to detect the vacuolating cytotoxin gene (vacA) type, the...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1999-04-01 00:00:00
abstract：:We analyze the experience in serologic diagnosis of Lyme's borreliosis. From a total of 551 patients studied from 1987 to 1989, we further evaluate 80 cases with erythema chronicum migrans or a clinical diagnosis of Lyme's disease and positive serological tests. The techniques used were IFI, ELISA1 (Whittaker Bioprodu...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1991-06-01 00:00:00
abstract：OBJECTIVE:Infectious pharyngotonsillitis is usually managed with antibiotics by general practitioners and pediatricians both in primary care and the emergency services. In the present work we try to assess the antibiotic variability and appropriateness in the management of acute pharyngotonsillitis among several emerge...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
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abstract：INTRODUCTION:Only automated phenotypic methods are currently used in Colombian hospitals for identifying isolates of the Acinetobacter calcoaceticus-A. baumannii complex (ACB). The phenotypical similarities in these species mean that they cannot be differentiated by manual or automated methods, thereby leading to their...
journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1016/j.eimc.2012.07.013
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abstract：INTRODUCTION:To describe demographic aspects of a prospective multicentric nationwide study on Escherichia coli and Klebsiella pneumoniae producing extended spectrum b -lactamases (ESBL) in Spain, between March and June 2000. METHODS:352 E. coli and K. pneumoniae strains showing a phenotype compatible to ESBL producti...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1016/j.eimc.2012.07.007
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journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2004-04-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1992-05-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 共识发展会议,杂志文章,实务指引
doi：10.1016/j.eimc.2015.03.020
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1016/s0213-005x(08)76567-4
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
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更新日期：2005-01-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
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journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 指南,杂志文章,实务指引
doi：
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：
更新日期：1995-02-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1157/13078799
更新日期：2005-08-01 00:00:00
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journal_title：Enfermedades infecciosas y microbiologia clinica
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doi：10.1016/j.eimc.2020.03.004
更新日期：2020-05-22 00:00:00
abstract：:The uncontrolled progression of the AIDS epidemic has made the development of an efficacious human immunodeficiency virus (HIV) vaccine a major objective of scientific research. No effective preventive vaccine against HIV is currently available and sterilizing immunity has not yet been achieved in animal models. This ...
journal_title：Enfermedades infecciosas y microbiologia clinica
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更新日期：2005-07-01 00:00:00
