[Typification of strains of Helicobacter pylori by the detection of the cagA gene and subtypes of the vacA gene].

Abstract:

BACKGROUND:Helicobacter pylori infection is probably the most common chronic bacterial infection in the world. The consequences of infection are pathologies like peptic ulcer, chronic gastritis, gastric cancer and gastric MALT lymphoma. The aim of this study was to detect the vacuolating cytotoxin gene (vacA) type, the cytotoxin-associated gen (cagA) of H. pylori isolates and study their association with the vacuolising activity. MATERIAL AND METHODS:Gastric biopsy specimens were obtained from dyspeptic patients. Isolates were further genotypically typed by PCR. RESULTS:All strains studied were vacA+ and 55% were cagA+. All cytotoxic strains were cagA+, subtype vacA s1/m1 and corresponded to patients with peptic ulceration. The cagA- strains (11) corresponded to subtype s2/m2. We didn't demonstrate vacuolising activity on subtypes s1/m2 and s2/m2. CONCLUSIONS:A high genetic diversity exists among strains in our environment. The subset of bacteria, vacA s1/m1/cagA+, is associated with vacuolising activity in culture cells (tox+).

authors

Gómez J,Elizalde JI,Marco F,Bordas JM,Piqué JM,Jiménez de Anta MT

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

171-5

issue

4

eissn

0213-005X

issn

1578-1852

journal_volume

17

pub_type

杂志文章
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    doi:

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    authors: Millan AB,Domínguez MA,Borraz C,González MP,Almirante B,Cercenado E,Padilla B,Pujol M,Rodríguez-Baño J,GEIH\/GEMARA\/REIPI.

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  • [Glycopeptide-resistant Enterococcus faecium. Analysis of the resistance genotype, virulence and genetic lines].

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