Abstract：
INTRODUCTION:A variable percentage of samples analysed using the Cobas 4800 assay can give an invalid result by PCR inhibition or erroneous due to incorrect DNA extraction with the Cobas 4800 CT/NG test. METHOD:An analysis was performed using the vortex agitation and dilution protocol on the original sample (swab or urine) for a total of 116 samples. In order to analyse the sensitivity of this method, 100 samples (swabs and urine) with known results were retested. RESULTS:A total of 98.3% (114/116) of the samples analysed were resolved with this protocol with 100% agreement after reviewing clinical data, Gram stain, and other samples analysed in parallel from the same patient. DISCUSSION:The data indicate no loss of sensitivity with this protocol; thus Cobas 4800 users could use this method without the need for alternative methods.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Parra-Sánchez M,García-Rey S,Zakariya-Yousef Breval I,Sierra-Atienza C,Bernal-Martínez S,Palomares-Folía JCdoi
10.1016/j.eimc.2015.06.017subject
Has Abstractpub_date
2017-01-01 00:00:00pages
364-366issue
6eissn
0213-005Xissn
1578-1852pii
S0213-005X(15)00275-Xjournal_volume
35pub_type
杂志文章abstract：BACKGROUND:Helicobacter pylori infection is probably the most common chronic bacterial infection in the world. The consequences of infection are pathologies like peptic ulcer, chronic gastritis, gastric cancer and gastric MALT lymphoma. The aim of this study was to detect the vacuolating cytotoxin gene (vacA) type, the...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：BACKGROUND:The aim of this study was to determine the incidence and antibiotic sensitivity of Neisseria gonorrhoeae versus penicillin, tetracycline, cefoxitin, ceftriaxone and spectinomycin from 1988 to 1994 in the province of Soria (Spain). METHODS:From January 1988 to December 1994, clinical samples of 57 strains of...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：OBJECTIVE:In 1995 a specialized home care service for infectious diseases was created in our institution. The aim was to improve the quality of life of patients with prolonged parenteral antimicrobial therapy requirements, reduce the length of hospital stay, and improve the care received after discharge by clinical and...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13108706
更新日期：2007-08-01 00:00:00
abstract：INTRODUCTION:Factors predicting short-term amputation during hospital treatment of patients admitted for acute diabetic foot infections are of interest for clinicians managing the acute episode. METHODS:A retrospective clinical records analysis of 78 consecutive patients hospitalized for acute diabetic foot infections...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2009.12.017
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Fecal colonization by multiresistant bacteria can be a source of nosocomial infections. The aim of this work was to study fecal colonization by Klebsiella pneumoniae in neonatal intensive care unit (NICU) patients and investigate the resistance profiles of the strains. METHODS:From May to October 2001, 11 s...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13067617
更新日期：2004-11-01 00:00:00
abstract：:Intestinal invagination is rather frequently associated with local tumors, adenovirus infections, and occasionally with Yersinia enterocolitica. However, in many cases the etiology is not known. We have studied 88 children with intestinal invagination in whom cellular cultures of Hep 2 and pulmonary fibroblasts of hum...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1990-08-01 00:00:00
abstract：BACKGROUND:Reflex testing is necessary to achieve the objectives of hepatitis C elimination. However, in 2017 only 31% of Spanish hospitals performed reflex test. As a consequence of that finding, reflex testing was recommended by scientific societies involved in the diagnosis and treatment of hepatitis C. OBJECTIVE:T...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2020.03.004
更新日期：2020-05-22 00:00:00
abstract：OBJECTIVE:To describe the changes in causes of death among persons with HIV infection. METHODS:An analysis of mortality according to cause was performed in persons diagnosed with HIV infection and residing in the province of Navarre (Spain) from 1985 to 2004. RESULTS:Among 1,649 persons diagnosed with HIV infection u...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13096746
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Visceral leishmaniasis (VL) is an endemic parasitosis in Spain with a frequency which is increasing, specially among those patients with HIV infection. METHODS:The clinical characteristics of 36 episodes of VL in 20 patients with HIV infection diagnosed in the authors' center from January 1988 to October 19...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：INTRODUCTION:There is scant information available in Spain regarding virological markers and clinical status in Sub-Saharan patients infected with HVB. METHODS:A cross-sectional and retrospective study of virological markers and clinical status of HBV infection in 510 adult patients from Sub-Saharan Africa, not co-inf...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2010.07.013
更新日期：2011-02-01 00:00:00
abstract：:The imipenem and meropenem breakpoints for Enterobacteriaceae established by the Clinical and Laboratory Standards Institute (CLSI) are somewhat lower than those established by the European Committee on Antimicrobial Susceptibility Testing (EUCAST), but are identical for ertapenem and doripenem. The differences are pr...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/S0213-005X(14)70172-7
更新日期：2014-12-01 00:00:00
abstract：:This review offers succinct, precise, and complete information based on the available data concerning new anaerobic bacterial species involved in infectious diseases in humans. All hitherto undescribed species, those not previously implicated in clinical conditions, those with confirmed implication in human disease th...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1016/j.eimc.2008.09.015
更新日期：2010-03-01 00:00:00
abstract：:The VINCat Program is a standardized surveillance program of healthcare infections in Catalonia, Spain. This program includes monitoring of surgical site infections (SSI) of elective colorectal surgery. The aim of this study was to define SSI rates in colorectal surgery among VINCat hospitals over a period of 4 years....
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/S0213-005X(12)70092-7
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:To determine variability, if exists, in interleukin-6 (IL-6) response by different cell cultures stimulated by Borrelia burgdorferi. METHODS:Five human embryonic diploid fibroblast cell lines (three of them of lung origin) and four human synovial cell cultures were used. Induction of IL-6 was made with B. b...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：INTRODUCTION:To describe demographic aspects of a prospective multicentric nationwide study on Escherichia coli and Klebsiella pneumoniae producing extended spectrum b -lactamases (ESBL) in Spain, between March and June 2000. METHODS:352 E. coli and K. pneumoniae strains showing a phenotype compatible to ESBL producti...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,多中心研究
doi：
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:To analyze the determinants that influence the health-related quality of life of people living with HIV in Alicante (Spain). METHODS:A cross-sectional study was conducted, which recruited 214 Spanish-speaking participants over 18 years of age living with HIV from an outpatient consulting office of the infectio...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2020.03.005
更新日期：2020-05-19 00:00:00
abstract：INTRODUCTION:the duration adequacy of antibiotic regimens is one of the key points of Antimicrobial Stewardship Programs (ASP) given the relationship between the risk of resistance and days of exposure to antimicrobials. METHODS:monitoring activities of intravenous antibiotics longer than 7 days at Hospital Infantil V...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2020.02.015
更新日期：2020-03-27 00:00:00
abstract：INTRODUCTION:To characterize OXA-48 carbapenemase-producing Klebsiella pneumoniae strains isolated after an increase in carbapenem resistance in Catalonia. METHODOLOGY:K. pneumoniae identification, antimicrobial susceptibility studies, the Modified Hodge Test method, amplification of antimicrobial resistance genes (ag...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/j.eimc.2018.02.003
更新日期：2019-02-01 00:00:00
abstract：:Heart transplantation has emerged as the therapy of choice for selected patients with life-threatening, irremediable heart disease. Infectious complications are frequent, but are no longer the leading cause of death. Late infections (particularly viral infections), however, are still a threat to long-term graft surviv...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：10.1157/13111187
更新日期：2007-11-01 00:00:00
abstract：:The diffusion of hepatitis B virus was investigated in an open institution for mentally retarded patients, where 24 patients with Down's syndrome (DS) and 94 with other types of mental retardation (OMR) were being cared for. The immunogenic response to a recombinant vaccine intramuscularly injected to the deltoid musc...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1990-03-01 00:00:00
abstract：:One of the current characteristics of migration is its tendency to concentrate in industrialized countries, as well as its feminization and diversity. From a healthcare point of view, the phenomenon of migration has aroused interest in the possible transfer of transmissible infectious diseases from some regions to oth...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13123261
更新日期：2008-05-01 00:00:00
abstract：:Intestinal parasite infections are very frequent in HIV patients with severe immunodeficiency (CD4 < 100/mm3) causing chronic diarrhea and malabsorption in the majority of cases. The most frequent microorganisms are microsporidia and Cryptosporidium parvum while Cyclospora cayetanensis and Isospora belli are more prev...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
abstract：:To date, more than 170 species of mycobacteria have been described, of which more than one third may be pathogenic to humans, representing a significant workload for microbiology laboratories. These species must be identified in clinical practice, which has long been a major problem due to the shortcomings of conventi...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/S0213-005X(16)30188-4
更新日期：2016-06-01 00:00:00
abstract：BACKGROUND:This review summarizes the different species of dermatophytes isolates in our laboratory between 1991 and 1995. We describe the clinical forms and establish the distribution over this period of time. METHODS:Retrospective survey of samples from outpatients of the Dermatology Service in Miguel Servet Hospita...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:The alarms that have sounded in the last two decades of the 20(th) century in the feld of antimicrobial resistance in livestock farming have led to the development of various measures that have substantially improved the use of antimicrobial agents in veterinary medicine. These measures mainly involve the implementati...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/S0213-005X(10)70042-2
更新日期：2010-11-01 00:00:00
abstract：:The fimbriated adhesines constitute a factor of virulence of E. coli in the urinary tract infections. The presence of type 1 fimbriae and fimbriae P was evaluated in 23 E. coli strains isolated with patients with chronic bacterial prostatitis. For the diagnosis of prostatitis the Stamey test was used. The detection of...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:Because lifelong therapy is needed in HIV infection, high long-term adherence is necessary. Darunavir/co-bicistat/emtricitabina/tenofovir alafenamide (Symtuza®) is the first triple therapy combining a protease inhibitor (PI) in a single tablet regimen. This drug combines the potency and high genetic barrier of the mos...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1016/S0213-005X(18)30395-1
更新日期：2018-12-01 00:00:00
abstract：:The evaluation and mechanism of protection against Y. enterocolitica O:8 using different immunization schedules. We immunize 3 groups of 20 mice each against an oral lethal dose of Y. enterocolitica O:8 using: 1) whole cells, 2) hyperimmune sera, and 3) LPS and common antigen. Animals immunized using whole live cells ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：INTRODUCTION:The aim of this study is to describe a Shigella sonnei outbreak in a school. METHOD:An epidemiological inquiry was performed. Stool samples from 5 patients were cultured. Molecular typing of the isolates was carried out by PFGE, MLST and plasmid analysis. RESULTS:The attack rate was 14.3% (67 students). ...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：10.1157/13100466
更新日期：2007-04-01 00:00:00
abstract：:In the present study the 60 patients with brucellosis and evaluation of bone marrow aspirate seen at the Hospital Base Cayetano Heredia from 1980 to 1986 were included. Iron deficiency was found in the bone marrow in 34.5% of patients, 31% in males and 36% in females. No correlation was found between iron deficiency a...
journal_title：Enfermedades infecciosas y microbiologia clinica
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
