Abstract:
:We evaluated 173 consecutive breast carcinomas for c-erbB-2 using a combination of immunohistochemistry (IHC) with a commercial polyclonal antibody (Nitirei) and dual-color fluorescence in situ hybridization (FISH) using the c-erbB-2-specific probe and the chromosome 17 centromere-specific probe from Vysis (Downers Grove, IL) and compared the results with the histologic characteristics of intraductal spread, cancer invasion, and intratumoral heterogeneity. With correction for chromosome 17 copy number, c-erbB-2 amplification was observed in 26 tumors (13.5%): high-level amplification in 23 tumors, and low-level amplification in 3. The gene amplification was positively correlated with c-erbB-2 protein overexpression, defined as 2+ or 3+ immunostaining, on a case-by-case basis (P < .000001). All 3+ immunostaining tumors (19 tumors) showed high-level amplification, although gene amplification was found in only 5 of 27 2+ immunostaining tumors. Although the rates of overexpression and gene amplification did not differ in ductal carcinomas in situ and invasive carcinomas (P = .46 and .53, respectively), they were significantly higher in invasive carcinomas with intraductal spreading (P < .0001). Intratumoral heterogeneity of c-erbB-2 amplification was found in only 1 case; however, in 17 invasive carcinomas, intraductal components expressed c-erbB-2 more intensely than invasive components. We conclude that in breast carcinomas, c-erbB-2 overexpression occurs mostly in tumors with high-level gene amplification, and such overexpression appears to endow carcinoma cells with the capacity for intraductal spreading. The best method for detecting breast carcinomas with c-erbB-2 aberrations using archival tissues is to screen cases by IHC; however, follow-up FISH assays are indispensable for excluding false-positive results.
journal_name
Hum Patholjournal_title
Human pathologyauthors
Kobayashi M,Ooi A,Oda Y,Nakanishi Idoi
10.1053/hupa.2002.30185subject
Has Abstractpub_date
2002-01-01 00:00:00pages
21-8issue
1eissn
0046-8177issn
1532-8392pii
S0046817702741804journal_volume
33pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract::Our previous investigations of transforming growth factor types beta 1 and beta 2 (TGF beta s) showed negative or positive autocrine growth regulation of gliomas in vitro. Near-diploid gliomas were inhibited by the TGF beta s, whereas a stimulatory response correlated with progressive anaplasia and karyotypic divergen...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(94)90118-x
更新日期:1994-05-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) plays a pivotal role in tumor invasion and metastasis in various malignancies. ZEB2/SIP1 is an important EMT regulator and down-regulates E-cadherin expression. The present study was planned to explore status of EMT-associated markers ZEB2/SIP1 and E-cadherin in eyelid sebaceous...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.05.026
更新日期:2015-10-01 00:00:00
abstract::Endolymphatic sac tumor (ELST) is a low-grade adenocarcinoma of the temporal bone that is presumed to originate from the endolymphatic system. Although ELSTs are extremely rare in the general population, a significant number of studies have documented the occurrence of ELST among patients with von Hippel-Lindau (VHL) ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.28961
更新日期:2001-11-01 00:00:00
abstract::Substantial agreement has previously been demonstrated between qualitative and morphometric grading of gastric dysplasia. In the present study, a further attempt is made to quantitatively define the dysplastic changes in relation to associated benign or malignant changes of gastric mucosa. In total, 232 cases were stu...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(89)90094-4
更新日期:1989-09-01 00:00:00
abstract::Recently, a novel tumor-associated protein, termed MN, has been described in carcinomas of the uterine cervix, where its expression has been shown to be associated with malignant transformation. Because malignant transformation in the esophagus develops through a dysplasia-carcinoma sequence similar to that which occu...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(97)90185-4
更新日期:1997-06-01 00:00:00
abstract::This article presents a view of lymphoid tissue architecture as defined by the traffic of defined lymphoid cell classes. The compartmentalization of lymphocytes is discussed in reference to specific cell-cell interactions that occur in antigen-driven immune responses. Finally, the distribution of normal and neoplastic...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/s0046-8177(78)80005-7
更新日期:1978-01-01 00:00:00
abstract::Gene amplification is a common event in breast cancer, and identifies actual and potential targets of molecular therapy. The aim of the present study was to determine the amplification status of 22 genes that are reportedly frequently amplified in breast cancers. An archive of 322 formalin-fixed and paraffin-embedded ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2018.10.017
更新日期:2019-03-01 00:00:00
abstract::The evolution of sequential histological changes from normal cells through invasive cancer affords the cancer biologist the opportunity to identify separate molecular steps involved in cancer progression. As one studies the development of human carcinoma, it becomes apparent that multiple genetic alterations affecting...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/0046-8177(94)90056-6
更新日期:1994-10-01 00:00:00
abstract::We report two cases of allergic bronchopulmonary fungal disease (ABPFD) caused by Curvularia sp and associated with allergic fungal sinusitis (AFS). Curvularia lunata was cultured in one case and Curvularia senegalensis was cultured in the other. Based on these cases and a review of the literature, we discuss unusual ...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/0046-8177(91)90106-y
更新日期:1991-12-01 00:00:00
abstract::A case of thymoma with associated opportunistic infections, CD4/CD8 T-lymphocyte imbalance, low CD4-positive T-lymphocyte counts and Kaposi's sarcoma (KS) without HIV infection is reported. Cytomegalovirus inclusions were identified in the nuclei of some KS spindle and endothelial cells. It is known that KS has a high...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(97)90261-6
更新日期:1997-10-01 00:00:00
abstract::CD34 has been widely used for the assessment of sinusoid-like neoangiogenesis in hepatocellular carcinoma (HCC). Recently, it was demonstrated that CD34-positive cells isolated from human peripheral blood differentiate into endothelial cells and contribute to neoangiogenesis in adults. We investigated the localization...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2001.29678
更新日期:2001-12-01 00:00:00
abstract::The epicardium contributes to cardiac formation, particularly during embryogenesis. It remains to be seen if it is also involved in postnatal myocardial homeostasis. This study evaluates the topographic distribution of stem cells (c-Kit) and extracardiac progenitor cells (CXCR4+) and their contribution to ventricular ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.12.017
更新日期:2010-11-01 00:00:00
abstract::Two episodes of severe, relapsing sensorimotor polyneuropathy closely paralleled the course of systemic illness in a patient with immunoblastic lymphadenopathy. A review of the literature shows three other cases, although none with documented pathologic changes in muscles and nerves. Based on the pathologic findings i...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(82)80120-2
更新日期:1982-02-01 00:00:00
abstract::The pertinent clinical data and histologic features of the testes in 57 autopsied acquired immunodeficiency syndrome (AIDS) patients were analyzed and compared with those of 55 age-matched control patients without AIDS. The testes of the AIDS patients showed a significantly lower degree of spermatogenesis (determined ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(89)90125-1
更新日期:1989-03-01 00:00:00
abstract::In the normal liver, the transforming growth factor beta (TGF-beta) signaling pathway plays an important role in inhibiting hepatocyte growth. This effect is mediated through Smad4 (or Dpc4), a tumor-suppressor gene that affects gene transcription and controls cell growth. A loss of Smad4 is associated with carcinoma ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2002.128061
更新日期:2002-09-01 00:00:00
abstract::Bone marrow biopsy findings in 32 homosexual patients with the acquired immunodeficiency syndrome (AIDS) or prodromal AIDS are described. A high incidence (38 per cent) of nonparatrabecular lymphohistiocytic infiltrations containing irregular small lymphocytes was observed, as well as the presence of diffuse bone marr...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(84)80248-8
更新日期:1984-11-01 00:00:00
abstract::Inflammatory bowel disease-associated colorectal carcinomas (IBD-CRCs) develop in a background of chronic inflammation, and thus, the molecular landscape of these tumors likely differs from that of sporadic colorectal cancer. To add to emerging data on molecular alterations present in these tumors, we analyzed our ins...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2019.04.013
更新日期:2019-07-01 00:00:00
abstract::Pilocytic astrocytoma is an infrequently encountered, generally low-grade neoplasm. No study has extensively looked at both cyclin D1 and MIB-1 labeling indices in pilocytic astrocytoma and their relation to clinical outcome. This study retrospectively examines the clinicopathologic features of 48 patients with pilocy...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(98)90023-5
更新日期:1998-12-01 00:00:00
abstract::We report the morphonuclear characteristics of normal (13 cases), benign (ie, chronic) pancreatitis (six cases), and neoplastic (ie, ductal) adenocarcinoma (22 cases) tissues of the pancreas. This description is based on computerized cell image analysis, which permits the determination of parameters related to the mor...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(92)90287-d
更新日期:1992-11-01 00:00:00
abstract::The Ewing family of tumors (EFT) is characterized by high MIC2/CD99 expression and specific EWS/ETS gene rearrangements, resulting in different chimeric transcripts. Further division into peripheral primitive neuroectodermal tumors and Ewing's sarcoma is still debated and, in the absence of distinct morphological para...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(99)90223-x
更新日期:1999-09-01 00:00:00
abstract::Selective expression of certain chemokine receptors by melanoma cells and the presence of their ligands in tissues might govern organ site-specific metastasis. Because the expression profile of chemokine receptors in tissues of melanocytic origin is unknown, we performed a comprehensive study on melanocytic tissue sam...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2006.11.013
更新日期:2007-05-01 00:00:00
abstract::A case of myocardial hyperplasia with numerous mitotic figures, caterpillar nuclei, and an abnormal DNA ploidy pattern is described in an 8-day-old full-term baby with uneventful gestation and sudden death. ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(92)90064-a
更新日期:1992-12-01 00:00:00
abstract::Mucus-filled cystic tumors of low or borderline malignant potential, well recognized in ovary and appendix, have received little attention in the lung. We present data on 11 patients, all of whom had solitary pulmonary nodules resected in which mucus was the major histologic component. Prognosis appears good; no patie...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90041-m
更新日期:1991-02-01 00:00:00
abstract::In a recent evaluation by comparative genomic hybridization, we demonstrated chromosome 14q31-32.1 to be frequently deleted in adenocarcinomas of the gastroesophageal junction. This suggests the presence of a tumor suppressor gene in the deleted region. In the present study, we have performed a detailed loss of hetero...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2005.12.010
更新日期:2006-05-01 00:00:00
abstract::Primitive neuroectodermal tumors are morphologically similar malignant tumors arising in intracranial and peripheral sites of the nervous system, showing varying degrees of cellular differentiation with a tendency to disseminate along cerebrospinal fluid pathways. They occur primarily in children and young adults. Und...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/s0046-8177(83)80006-9
更新日期:1983-06-01 00:00:00
abstract::The ontogeny and distribution of the epidermal growth factor (EGF) receptor and lipocortin-1, a major cellular substrate of the EGF receptor, were evaluated in a developmental series of fetal and neonatal human lungs (8 to 41 weeks' gestation and stillborn to 16 days' postnatal age). The peroxidase anti-peroxidase tec...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(90)90127-q
更新日期:1990-02-01 00:00:00
abstract::A solitary fibrous tumor (SFT) is a rare, NAB2-STAT6 fusion gene-associated mesenchymal neoplasm. It most commonly arises in the pleural site, but it can occur at many other sites, and rarely also in the head and neck (H&N) region. STFs may show many growth patterns and therefore can be easily mistaken for other more ...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/j.humpath.2020.03.007
更新日期:2020-05-01 00:00:00
abstract::To assess the proliferative activity of renal cell carcinoma (RCC-A) in patients with acquired cystic disease of the kidney (ACDK) after long-term hemodialysis, we analyzed cell cycle, DNA ploidy, and S-phase fraction by flow cytometry (FCM) and proliferating cell nuclear antigen (PCNA) labeling index by immunohistoch...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2002.30188
更新日期:2002-02-01 00:00:00
abstract::Primary carcinomas of the urethra are rare and poorly understood lesions; hence, their clinical and pathologic spectrum is not completely defined. We analyzed a series of 130 primary urethral tumors and classified 106 of them as primary urethral carcinomas. The age at diagnosis of patients with primary urethral carcin...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.08.006
更新日期:2018-02-01 00:00:00
abstract::Primary renal collision tumors composed of 2 histologically distinct components are rare with only isolated case reports in the medical literature. Oncocytoma is a benign renal neoplasm, which is thought to originate from distal tubular epithelial cells. Mucinous tubular and spindle cell carcinoma (MTSCC) of kidney is...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.08.012
更新日期:2018-01-01 00:00:00