Abstract:
:We studied gamma-aminobutyric acid (GABA)-mediated currents in short-term cultured dorsal unpaired median (DUM) neurons of cockroach Periplaneta americana using the whole cell patch-clamp technique in symmetrical chloride solutions. All DUM neurons voltage-clamped at -50 mV displayed inward currents (I(GABA)) when 10(-4) M of GABA was applied by pneumatic pressure-ejection pulses. The semi-logarithmic curve of I(GABA) amplitude versus the ejection time yielded a Hill coefficient of 4.0. I(GABA) was chloride (Cl-) because the reversal potential given by the current-voltage (I-V) curve varied according to the value predicted by the Nernst equation for Cl- dependence. In addition, I(GABA) was almost completely blocked by bath application of the chloride channel blockers picrotoxin (PTX) or 3,3-bis(trifluoromethyl)bicyclo-[2,2,1]heptane-2,2-diacarbonitrile (BIDN). The I-V curve for I(GABA) displayed a unexpected biphasic aspect and was best fitted by two linear regressions giving two slope conductances of 35.6 +/- 2.1 and 80.9 +/- 4.1 nS for potentials ranging from 0 to -30 and -30 to -70 mV, respectively. At -50 mV, the current amplitude was decreased by cadmium chloride (CdCl2, 10(-3) M) and calcium-free solution. The semi-logarithmic curve for CdCl2-resistant I(GABA) gave a Hill coefficient of 2.4. Hyperpolarizing voltage step from -50 to -80 mV was known to increase calcium influx through calcium-resting channels. According to this protocol, a significant increase of I(GABA) amplitude was observed. However, this effect was never obtained when the same protocol was applied on cell body pretreated with CdCl2. When the calmodulin blocker N-(6-aminohexyl)-5-chloro-1-naphtalene-sulfonamide or the calcium-calmodulin-dependent protein kinase blocker 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN-62) was added in the pipette solution, I(GABA) amplitude was decreased. Pressure ejection application of the cis-4-aminocrotonic acid (CACA) on DUM neuron cell body held at -50 mV, evoked a Cl- inward current which was insensitive to CdCl2. The Hill plot yielded a Hill coefficient of 2.3, and the I-V curve was always linear in the negative potential range with a slope conductance of 32.4 +/- 1.1 nS. These results, similar to those obtained with GABA in the presence of CdCl2 and KN-62, indicated that CACA activated one subtype of GABA receptor. Our study demonstrated that at least two distinct subtypes of Cl--dependent GABA receptors were expressed in DUM neurons, one of which is regulated by an intracellular Ca2+-dependent mechanism via a calcium-dependent protein kinase. The consequences of the modulatory action of Ca2+ in GABA receptors function and their sensitivity to insecticide are discussed.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Alix P,Grolleau F,Hue Bdoi
10.1152/jn.2002.87.6.2972subject
Has Abstractpub_date
2002-06-01 00:00:00pages
2972-82issue
6eissn
0022-3077issn
1522-1598journal_volume
87pub_type
杂志文章abstract::The recurrent mossy fiber pathway of the dentate gyrus expands dramatically in many persons with temporal lobe epilepsy. The new connections among granule cells provide a novel mechanism of synchronization that could enhance the participation of these cells in seizures. Despite the presence of robust recurrent mossy f...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.5.2380
更新日期:2000-11-01 00:00:00
abstract::The functional role of sensory input to the motor cortex was studied by interrupting two major input pathways. One was the dorsal column, which sends the input directly through the thalamus to the motor cortex, and the other was the sensory cortex, which transfers its input through association fibers. Removal of the s...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.52.2.212
更新日期:1984-08-01 00:00:00
abstract::Through their actions in the nucleus accumbens (NAc), kappa opioid (KOP) receptors and their endogenous ligand, dynorphin, modify behaviors associated with the administration of drugs of abuse and are regulated by exposure to such drugs. Despite their demonstrated behavioral significance, the synaptic actions of KOP r...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01115.2002
更新日期:2003-05-01 00:00:00
abstract::1. Long-term depression (LTD) is an activity-dependent reduction in the strength of synaptic transmission that can persist for hours. It is a neural model for processes underlying learning and memory, such as extinction and forgetting. LTD of excitatory postsynaptic potentials (EPSPs) in cells of the CA1 region of hip...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.69.1.219
更新日期:1993-01-01 00:00:00
abstract::Mechanosensory neurons exhibit a wide range of dynamic changes in response, including rapid and slow adaptation. In addition to mechanical factors, electrical processes may also contribute to sensory adaptation. We have investigated adaptation of afferent neurons in the stick insect femoral chordotonal organ (fCO). Th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00339.2002
更新日期:2002-11-01 00:00:00
abstract::1. The fast extensor tibiae motor neuron makes direct excitatory central connections with the posterior group of flexor tibiae motor neurons in the locust metathoracic ganglion. The flexor group has a slow, a fast, and an intermediate motor neuron. The motor neurons are involved in the motor program for kicking and ju...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.2.529
更新日期:1995-08-01 00:00:00
abstract::When the brain has determined the position of a moving object, because of anatomical and processing delays the object will have already moved to a new location. Given the statistical regularities present in natural motion, the brain may have acquired compensatory mechanisms to minimize the mismatch between the perceiv...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00792.2017
更新日期:2018-11-01 00:00:00
abstract::Action potential initiation and conduction along peripheral axons is a dynamic process that displays pronounced activity dependence. In patients with neuropathic pain, differences in the modulation of axonal conduction velocity by activity suggest that this property may provide insight into some of the pathomechanisms...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00777.2012
更新日期:2014-05-01 00:00:00
abstract::The ventrolateral preoptic nucleus (VLPO) is a key nucleus involved in the homeostatic regulation of sleep-wakefulness. Little is known, however, about the cellular mechanisms underlying its role in sleep regulation and how the neurotransmitters, such as GABA and noradrenaline (NA), are involved. In the present study ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00491.2002
更新日期:2003-03-01 00:00:00
abstract::1. Adaptation and disadaptation rates determine the temporal response properties of sensory receptor cells. In chemoreception, temporal filter properties of receptor cells are poorly understood. We studied the time course of disadaptation in lobster antennular chemoreceptor cells by using in situ high-resolution stimu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.2.1340
更新日期:1996-08-01 00:00:00
abstract::To gain further insight into the neural mechanisms for different forms of quadrupedal walking, data on postural orientation, hindlimb kinematics, and motor patterns were assessed for four grades of downslope walking, from 25% (14 degrees slope) to 100% (45 degrees), and compared with data from level and downslope walk...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.4.1702
更新日期:1998-04-01 00:00:00
abstract::1. The synaptic pathways underlying tectal influence over pinna movements were studied using an acute electrophysiological approach. Under pentobarbital anesthesia, postsynaptic potentials were recorded intracellularly in antidromically identified, cat facial motoneurons following electrical stimulation of the superio...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.60.2.769
更新日期:1988-08-01 00:00:00
abstract::Upon prolonged viewing of a sinusoidal grating, the visual system is selectively desensitized to the spatial frequency of the grating, while the sensitivity to other spatial frequencies remains largely unaffected. This technique, known as pattern adaptation, has been so central to the psychophysical study of the mecha...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00216.2009
更新日期:2009-10-01 00:00:00
abstract::The relative motion between the surface of an object and our fingers produces patterns of skin deformation such as stretch, indentation, and vibrations. In this study, we hypothesized that motion-induced vibrations are combined with other tactile cues for the discrimination of tactile speed. Specifically, we hypothesi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00621.2015
更新日期:2015-12-01 00:00:00
abstract::The functional maturation of cuneate neurons was studied using reproducible tactile stimulation procedures and quantitative assessment of coding capacities in anesthetized (N2O/O2 plus barbiturate infusion) or decerebrate cats from six different age groups; neonatal (1-5 days), 10-15, 25-30, 55-63, and 83-90 days, and...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.52.1.86
更新日期:1984-07-01 00:00:00
abstract::In songbirds, the basal ganglia outflow nucleus LMAN is a cortical analog that is required for several forms of song plasticity and learning. Moreover, in adults, inactivating LMAN can reverse the initial expression of learning driven via aversive reinforcement. In the present study, we investigated how LMAN contribut...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00311.2011
更新日期:2011-10-01 00:00:00
abstract::Reach-to-grasp movements require information about the distance and size of target objects. Calibration of this information could be achieved via feedback information (visual and/or haptic) regarding terminal accuracy when target objects are grasped. A number of reports suggest that the nervous system alters reach-to-...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00112.2013
更新日期:2013-12-01 00:00:00
abstract::Thalamocortical neurons integrate sensory and cortical activity and are regulated by input from inhibitory neurons in the thalamic reticular nucleus. Evidence suggests that during bursts of action potentials, dendritic calcium transients are seen throughout the dendritic tree of thalamocortical cells. Here, we review ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章,评审
doi:10.1152/jn.00918.2013
更新日期:2014-11-01 00:00:00
abstract::In quadrupeds, the dorsal-side-up body orientation during standing is maintained due to a postural system that is driven by feedback signals coming mainly from limb mechanoreceptors. In caudally decerebrated (postmammillary) rabbits, the efficacy of this system is considerably reduced. In this paper, we report that th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00042.2012
更新日期:2012-06-01 00:00:00
abstract::Ablation and single-unit studies in primates have shown that inferior temporal (IT) cortex is important for pattern discrimination. The first paper in this series suggested that single units in IT cortex of alert monkeys respond to a set of two-dimensional patterns with complex temporal modulation of their spike train...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.1.162
更新日期:1987-01-01 00:00:00
abstract::In addition to a primary somatosensory cortex (SI), the cerebral cortex of all mammals contains a second somatosensory area (SII); however, the functions of SII are largely unknown. Our aim was to explore the functions of SII by comparing response properties of whisker-related neurons in this area with their counterpa...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00262.2004
更新日期:2004-10-01 00:00:00
abstract::1. A reverse correlation (6, 8, 25, 35) method is developed that allows quantitative determination of visual receptive-field structure in two spatial dimensions. This method is applied to simple cells in the cat striate cortex. 2. It is demonstrated that the reverse correlation method yields results with several desir...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.58.6.1187
更新日期:1987-12-01 00:00:00
abstract::Moth pheromones cause rises in intracellular Ca(2+) concentrations that activate Ca(2+)-dependent cation channels in antennal olfactory receptor neurons. In addition, mechanisms of adaptation and sensitization depend on changes in cyclic nucleotide concentrations. Here, cyclic nucleotide-activated currents in cultured...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01400.2007
更新日期:2008-11-01 00:00:00
abstract::Ocular following (OFR) is a short-latency visual stabilization response to the optic flow experienced during self-motion. It has been proposed that it represents the early component of optokinetic nystagmus (OKN) and that it is functionally linked to the vestibularly driven stabilization reflex during translation (tra...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01020.2004
更新日期:2005-03-01 00:00:00
abstract::Ipsilaterally projecting spinal excitatory interneurons (EINs) generate the hemisegmental rhythmic locomotor activity in lamprey, while the commissural interneurons ensure proper left-right alternation. 5-HT is a potent modulator of the locomotor rhythm and is endogenously released from the spinal cord during fictive ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00309.2006
更新日期:2006-08-01 00:00:00
abstract::For primary auditory cortex (AI) laminae, there is little evidence of functional specificity despite clearly expressed cellular and connectional differences. Natural sounds are dominated by dynamic temporal and spectral modulations and we used these properties to evaluate local functional differences or constancies ac...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00624.2009
更新日期:2010-01-01 00:00:00
abstract::1. Spontaneous saccades, vestibuloocular responses (VOR), and optokinetic nystagmus were recorded in three squirrel monkeys before and after chemical or electrolytic lesion of the posterior commissure (PC). 2. PC lesions produced abnormal vertical eye movements, in particular, 1) Postsaccadic drifts, and 2) VOR gain r...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.6.2582
更新日期:1994-06-01 00:00:00
abstract::Certain hand-held tools alter the mapping between hand motion and motion of the tool end point that must be controlled in order to perform a task. For example, when using a pool cue, the motion of the cue tip is reversed relative to the hand. Previous studies have shown that the time required to initiate a reaching mo...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00157.2012
更新日期:2012-10-01 00:00:00
abstract::Motor-unit synchronization, which is a measure of the near simultaneous discharge of action potentials by motor units, has the potential to influence spike-triggered average force and the steadiness of a low-force isometric contraction. The purpose of the study was to estimate the contribution of motor-unit synchroniz...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.84.1.358
更新日期:2000-07-01 00:00:00
abstract::In this study we attempted to identify the principles that govern the changes in neural control that occur during repeated performance of a multiarticular coordination task. Eight participants produced isometric flexion/extension and pronation/supination torques at the radiohumeral joint, either in isolation (e.g., fl...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章
doi:10.1152/jn.00670.2004
更新日期:2005-11-01 00:00:00